Strain Fitness in Pectobacterium carotovorum WPP14 around HER17_RS03815

Experiment: M9+glycerol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHER17_RS03805 and HER17_RS03810 are separated by 101 nucleotidesHER17_RS03810 and HER17_RS03815 are separated by 21 nucleotidesHER17_RS03815 and HER17_RS03820 overlap by 32 nucleotides HER17_RS03805: HER17_RS03805 - pyrroline-5-carboxylate reductase, at 860,688 to 861,509 _RS03805 HER17_RS03810: HER17_RS03810 - YggS family pyridoxal phosphate-dependent enzyme, at 861,611 to 862,324 _RS03810 HER17_RS03815: HER17_RS03815 - type IV pilus twitching motility protein PilT, at 862,346 to 863,362 _RS03815 HER17_RS03820: HER17_RS03820 - diguanylate cyclase, at 863,331 to 865,163 _RS03820 Position (kb) 862 863 864Strain fitness (log2 ratio) -2 -1 0 1 2at 861.365 kb on - strand, within HER17_RS03805at 861.498 kb on - strandat 861.542 kb on - strandat 861.542 kb on - strandat 861.608 kb on + strandat 861.609 kb on - strandat 861.613 kb on - strandat 861.614 kb on + strandat 861.614 kb on + strandat 861.630 kb on + strandat 861.673 kb on + strandat 861.674 kb on - strandat 861.674 kb on - strandat 861.727 kb on + strand, within HER17_RS03810at 861.728 kb on - strand, within HER17_RS03810at 861.733 kb on + strand, within HER17_RS03810at 861.733 kb on + strand, within HER17_RS03810at 861.733 kb on + strand, within HER17_RS03810at 861.733 kb on + strand, within HER17_RS03810at 861.734 kb on - strand, within HER17_RS03810at 861.734 kb on - strand, within HER17_RS03810at 861.734 kb on - strand, within HER17_RS03810at 861.734 kb on - strand, within HER17_RS03810at 861.734 kb on - strand, within HER17_RS03810at 861.734 kb on - strand, within HER17_RS03810at 861.734 kb on - strand, within HER17_RS03810at 861.902 kb on - strand, within HER17_RS03810at 861.931 kb on + strand, within HER17_RS03810at 861.932 kb on - strand, within HER17_RS03810at 861.932 kb on - strand, within HER17_RS03810at 861.932 kb on - strand, within HER17_RS03810at 862.057 kb on + strand, within HER17_RS03810at 862.058 kb on - strand, within HER17_RS03810at 862.122 kb on + strand, within HER17_RS03810at 862.152 kb on + strand, within HER17_RS03810at 862.153 kb on - strand, within HER17_RS03810at 862.153 kb on - strand, within HER17_RS03810at 862.153 kb on - strand, within HER17_RS03810at 862.153 kb on - strand, within HER17_RS03810at 862.153 kb on - strand, within HER17_RS03810at 862.153 kb on - strand, within HER17_RS03810at 862.153 kb on - strand, within HER17_RS03810at 862.232 kb on + strand, within HER17_RS03810at 862.238 kb on - strand, within HER17_RS03810at 862.238 kb on - strand, within HER17_RS03810at 862.302 kb on - strandat 862.302 kb on - strandat 862.329 kb on - strandat 862.329 kb on - strandat 862.345 kb on + strandat 862.346 kb on - strandat 862.373 kb on - strandat 862.384 kb on + strandat 862.384 kb on + strandat 862.384 kb on + strandat 862.385 kb on - strandat 862.429 kb on - strandat 862.446 kb on + strandat 862.446 kb on + strandat 862.459 kb on + strand, within HER17_RS03815at 862.464 kb on + strand, within HER17_RS03815at 862.474 kb on - strand, within HER17_RS03815at 862.474 kb on - strand, within HER17_RS03815at 862.674 kb on + strand, within HER17_RS03815at 862.674 kb on + strand, within HER17_RS03815at 862.674 kb on + strand, within HER17_RS03815at 862.675 kb on - strand, within HER17_RS03815at 862.675 kb on - strand, within HER17_RS03815at 862.675 kb on - strand, within HER17_RS03815at 862.816 kb on + strand, within HER17_RS03815at 862.970 kb on - strand, within HER17_RS03815at 863.036 kb on - strand, within HER17_RS03815at 863.187 kb on - strand, within HER17_RS03815at 863.218 kb on + strand, within HER17_RS03815at 863.306 kb on + strandat 863.307 kb on - strandat 863.307 kb on - strandat 863.332 kb on + strandat 863.332 kb on + strandat 863.368 kb on + strandat 863.369 kb on - strandat 863.369 kb on - strandat 863.507 kb on + strandat 863.537 kb on - strand, within HER17_RS03820at 863.537 kb on - strand, within HER17_RS03820at 863.537 kb on - strand, within HER17_RS03820at 863.554 kb on - strand, within HER17_RS03820at 863.554 kb on - strand, within HER17_RS03820at 863.554 kb on - strand, within HER17_RS03820at 863.665 kb on + strand, within HER17_RS03820at 863.841 kb on + strand, within HER17_RS03820at 863.842 kb on - strand, within HER17_RS03820at 863.842 kb on - strand, within HER17_RS03820at 863.842 kb on - strand, within HER17_RS03820at 863.913 kb on + strand, within HER17_RS03820at 863.913 kb on + strand, within HER17_RS03820at 863.990 kb on + strand, within HER17_RS03820at 864.014 kb on - strand, within HER17_RS03820at 864.014 kb on - strand, within HER17_RS03820at 864.014 kb on - strand, within HER17_RS03820at 864.014 kb on - strand, within HER17_RS03820at 864.015 kb on + strand, within HER17_RS03820at 864.016 kb on - strand, within HER17_RS03820at 864.016 kb on - strand, within HER17_RS03820at 864.016 kb on - strand, within HER17_RS03820at 864.016 kb on - strand, within HER17_RS03820

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Per-strain Table

Position Strand Gene LocusTag Fraction M9+glycerol
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861,365 - HER17_RS03805 0.82 -0.8
861,498 - -1.3
861,542 - +1.5
861,542 - -0.0
861,608 + -0.1
861,609 - -0.5
861,613 - -0.9
861,614 + +0.5
861,614 + -0.2
861,630 + -0.3
861,673 + +1.2
861,674 - -0.3
861,674 - -0.5
861,727 + HER17_RS03810 0.16 +0.5
861,728 - HER17_RS03810 0.16 -0.3
861,733 + HER17_RS03810 0.17 -0.6
861,733 + HER17_RS03810 0.17 -1.1
861,733 + HER17_RS03810 0.17 +0.5
861,733 + HER17_RS03810 0.17 +1.9
861,734 - HER17_RS03810 0.17 +0.6
861,734 - HER17_RS03810 0.17 +0.5
861,734 - HER17_RS03810 0.17 -0.0
861,734 - HER17_RS03810 0.17 -0.6
861,734 - HER17_RS03810 0.17 +0.7
861,734 - HER17_RS03810 0.17 -0.6
861,734 - HER17_RS03810 0.17 -1.3
861,902 - HER17_RS03810 0.41 -0.2
861,931 + HER17_RS03810 0.45 +0.1
861,932 - HER17_RS03810 0.45 -1.7
861,932 - HER17_RS03810 0.45 +0.5
861,932 - HER17_RS03810 0.45 -0.7
862,057 + HER17_RS03810 0.62 -0.4
862,058 - HER17_RS03810 0.63 -0.3
862,122 + HER17_RS03810 0.72 -0.0
862,152 + HER17_RS03810 0.76 +0.7
862,153 - HER17_RS03810 0.76 -1.1
862,153 - HER17_RS03810 0.76 -0.1
862,153 - HER17_RS03810 0.76 +1.1
862,153 - HER17_RS03810 0.76 -0.4
862,153 - HER17_RS03810 0.76 +0.2
862,153 - HER17_RS03810 0.76 -0.6
862,153 - HER17_RS03810 0.76 -0.1
862,232 + HER17_RS03810 0.87 -0.6
862,238 - HER17_RS03810 0.88 -0.3
862,238 - HER17_RS03810 0.88 -0.0
862,302 - +0.4
862,302 - -0.1
862,329 - -0.0
862,329 - +0.3
862,345 + +0.1
862,346 - +0.0
862,373 - -0.7
862,384 + +0.1
862,384 + -0.4
862,384 + -0.4
862,385 - +0.7
862,429 - +0.2
862,446 + +0.2
862,446 + +0.2
862,459 + HER17_RS03815 0.11 +0.6
862,464 + HER17_RS03815 0.12 +0.9
862,474 - HER17_RS03815 0.13 -2.7
862,474 - HER17_RS03815 0.13 -2.1
862,674 + HER17_RS03815 0.32 +0.4
862,674 + HER17_RS03815 0.32 +0.5
862,674 + HER17_RS03815 0.32 +0.9
862,675 - HER17_RS03815 0.32 +0.4
862,675 - HER17_RS03815 0.32 -0.6
862,675 - HER17_RS03815 0.32 -0.1
862,816 + HER17_RS03815 0.46 -1.5
862,970 - HER17_RS03815 0.61 +0.1
863,036 - HER17_RS03815 0.68 +0.5
863,187 - HER17_RS03815 0.83 +0.3
863,218 + HER17_RS03815 0.86 -0.3
863,306 + -0.4
863,307 - -0.9
863,307 - -0.5
863,332 + +0.5
863,332 + +0.2
863,368 + -1.2
863,369 - -0.2
863,369 - +0.1
863,507 + +0.3
863,537 - HER17_RS03820 0.11 -0.8
863,537 - HER17_RS03820 0.11 -0.8
863,537 - HER17_RS03820 0.11 -0.9
863,554 - HER17_RS03820 0.12 -0.4
863,554 - HER17_RS03820 0.12 +0.9
863,554 - HER17_RS03820 0.12 -0.2
863,665 + HER17_RS03820 0.18 +1.3
863,841 + HER17_RS03820 0.28 +0.3
863,842 - HER17_RS03820 0.28 -0.2
863,842 - HER17_RS03820 0.28 +0.5
863,842 - HER17_RS03820 0.28 -1.1
863,913 + HER17_RS03820 0.32 -0.5
863,913 + HER17_RS03820 0.32 +0.0
863,990 + HER17_RS03820 0.36 +1.1
864,014 - HER17_RS03820 0.37 +0.9
864,014 - HER17_RS03820 0.37 -0.8
864,014 - HER17_RS03820 0.37 +0.8
864,014 - HER17_RS03820 0.37 -0.2
864,015 + HER17_RS03820 0.37 +0.5
864,016 - HER17_RS03820 0.37 -0.2
864,016 - HER17_RS03820 0.37 -0.6
864,016 - HER17_RS03820 0.37 +0.4
864,016 - HER17_RS03820 0.37 -0.4

Or see this region's nucleotide sequence