Strain Fitness in Pectobacterium carotovorum WPP14 around HER17_RS12245

Experiment: M9+glycerol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHER17_RS12240 and HER17_RS12245 overlap by 1 nucleotidesHER17_RS12245 and HER17_RS12250 are separated by 6 nucleotidesHER17_RS12250 and HER17_RS12255 overlap by 19 nucleotides HER17_RS12240: HER17_RS12240 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,729,756 to 2,730,823 _RS12240 HER17_RS12245: HER17_RS12245 - imidazole glycerol phosphate synthase subunit HisH, at 2,730,823 to 2,731,413 _RS12245 HER17_RS12250: HER17_RS12250 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,731,420 to 2,732,157 _RS12250 HER17_RS12255: HER17_RS12255 - imidazole glycerol phosphate synthase subunit HisF, at 2,732,139 to 2,732,915 _RS12255 Position (kb) 2730 2731 2732Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2729.899 kb on + strand, within HER17_RS12240at 2730.029 kb on - strand, within HER17_RS12240at 2730.095 kb on + strand, within HER17_RS12240at 2730.096 kb on - strand, within HER17_RS12240at 2730.127 kb on + strand, within HER17_RS12240at 2730.127 kb on + strand, within HER17_RS12240at 2730.128 kb on - strand, within HER17_RS12240at 2730.131 kb on + strand, within HER17_RS12240at 2730.131 kb on + strand, within HER17_RS12240at 2730.132 kb on - strand, within HER17_RS12240at 2730.132 kb on - strand, within HER17_RS12240at 2730.197 kb on + strand, within HER17_RS12240at 2730.230 kb on - strand, within HER17_RS12240at 2730.300 kb on + strand, within HER17_RS12240at 2730.300 kb on + strand, within HER17_RS12240at 2730.300 kb on + strand, within HER17_RS12240at 2730.301 kb on - strand, within HER17_RS12240at 2730.301 kb on - strand, within HER17_RS12240at 2730.427 kb on - strand, within HER17_RS12240at 2730.427 kb on - strand, within HER17_RS12240at 2730.427 kb on - strand, within HER17_RS12240at 2730.427 kb on - strand, within HER17_RS12240at 2730.428 kb on + strand, within HER17_RS12240at 2730.428 kb on + strand, within HER17_RS12240at 2730.429 kb on - strand, within HER17_RS12240at 2730.429 kb on - strand, within HER17_RS12240at 2730.451 kb on - strand, within HER17_RS12240at 2730.457 kb on + strand, within HER17_RS12240at 2730.488 kb on - strand, within HER17_RS12240at 2730.674 kb on + strand, within HER17_RS12240at 2730.688 kb on + strand, within HER17_RS12240at 2730.706 kb on + strand, within HER17_RS12240at 2730.706 kb on + strand, within HER17_RS12240at 2730.794 kb on - strandat 2730.875 kb on - strandat 2730.897 kb on + strand, within HER17_RS12245at 2730.898 kb on - strand, within HER17_RS12245at 2730.898 kb on - strand, within HER17_RS12245at 2730.908 kb on + strand, within HER17_RS12245at 2731.234 kb on + strand, within HER17_RS12245at 2731.235 kb on - strand, within HER17_RS12245at 2731.255 kb on + strand, within HER17_RS12245at 2731.255 kb on + strand, within HER17_RS12245at 2731.255 kb on + strand, within HER17_RS12245at 2731.255 kb on + strand, within HER17_RS12245at 2731.255 kb on + strand, within HER17_RS12245at 2731.256 kb on - strand, within HER17_RS12245at 2731.256 kb on - strand, within HER17_RS12245at 2731.256 kb on - strand, within HER17_RS12245at 2731.256 kb on - strand, within HER17_RS12245at 2731.412 kb on - strandat 2731.436 kb on + strandat 2731.436 kb on + strandat 2731.436 kb on + strandat 2731.437 kb on - strandat 2731.613 kb on + strand, within HER17_RS12250at 2731.694 kb on + strand, within HER17_RS12250at 2731.694 kb on + strand, within HER17_RS12250at 2731.694 kb on + strand, within HER17_RS12250at 2731.695 kb on - strand, within HER17_RS12250at 2731.898 kb on - strand, within HER17_RS12250at 2731.898 kb on - strand, within HER17_RS12250at 2731.898 kb on - strand, within HER17_RS12250at 2731.979 kb on - strand, within HER17_RS12250at 2732.201 kb on - strandat 2732.286 kb on + strand, within HER17_RS12255at 2732.291 kb on + strand, within HER17_RS12255at 2732.291 kb on + strand, within HER17_RS12255at 2732.325 kb on - strand, within HER17_RS12255

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Per-strain Table

Position Strand Gene LocusTag Fraction M9+glycerol
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2,729,899 + HER17_RS12240 0.13 -3.5
2,730,029 - HER17_RS12240 0.26 -3.2
2,730,095 + HER17_RS12240 0.32 -2.7
2,730,096 - HER17_RS12240 0.32 -4.7
2,730,127 + HER17_RS12240 0.35 -3.3
2,730,127 + HER17_RS12240 0.35 -3.3
2,730,128 - HER17_RS12240 0.35 -3.1
2,730,131 + HER17_RS12240 0.35 -2.0
2,730,131 + HER17_RS12240 0.35 -1.9
2,730,132 - HER17_RS12240 0.35 -4.4
2,730,132 - HER17_RS12240 0.35 -2.0
2,730,197 + HER17_RS12240 0.41 -3.1
2,730,230 - HER17_RS12240 0.44 -3.8
2,730,300 + HER17_RS12240 0.51 -2.1
2,730,300 + HER17_RS12240 0.51 -1.7
2,730,300 + HER17_RS12240 0.51 -1.1
2,730,301 - HER17_RS12240 0.51 -2.5
2,730,301 - HER17_RS12240 0.51 -2.6
2,730,427 - HER17_RS12240 0.63 -3.0
2,730,427 - HER17_RS12240 0.63 -2.5
2,730,427 - HER17_RS12240 0.63 -1.8
2,730,427 - HER17_RS12240 0.63 -3.1
2,730,428 + HER17_RS12240 0.63 -4.6
2,730,428 + HER17_RS12240 0.63 -1.6
2,730,429 - HER17_RS12240 0.63 -1.6
2,730,429 - HER17_RS12240 0.63 -2.1
2,730,451 - HER17_RS12240 0.65 -1.7
2,730,457 + HER17_RS12240 0.66 -3.6
2,730,488 - HER17_RS12240 0.69 -4.3
2,730,674 + HER17_RS12240 0.86 -3.7
2,730,688 + HER17_RS12240 0.87 -2.1
2,730,706 + HER17_RS12240 0.89 -1.4
2,730,706 + HER17_RS12240 0.89 -2.8
2,730,794 - -1.1
2,730,875 - -1.7
2,730,897 + HER17_RS12245 0.13 -2.0
2,730,898 - HER17_RS12245 0.13 -2.9
2,730,898 - HER17_RS12245 0.13 -4.6
2,730,908 + HER17_RS12245 0.14 -2.7
2,731,234 + HER17_RS12245 0.70 -1.7
2,731,235 - HER17_RS12245 0.70 -2.7
2,731,255 + HER17_RS12245 0.73 -2.3
2,731,255 + HER17_RS12245 0.73 -1.7
2,731,255 + HER17_RS12245 0.73 -2.5
2,731,255 + HER17_RS12245 0.73 -4.5
2,731,255 + HER17_RS12245 0.73 -0.1
2,731,256 - HER17_RS12245 0.73 -4.5
2,731,256 - HER17_RS12245 0.73 -1.6
2,731,256 - HER17_RS12245 0.73 -2.0
2,731,256 - HER17_RS12245 0.73 -2.9
2,731,412 - -4.9
2,731,436 + -3.2
2,731,436 + -3.7
2,731,436 + -2.0
2,731,437 - -2.0
2,731,613 + HER17_RS12250 0.26 -2.0
2,731,694 + HER17_RS12250 0.37 -4.4
2,731,694 + HER17_RS12250 0.37 -2.1
2,731,694 + HER17_RS12250 0.37 -2.4
2,731,695 - HER17_RS12250 0.37 -3.4
2,731,898 - HER17_RS12250 0.65 -2.6
2,731,898 - HER17_RS12250 0.65 -4.2
2,731,898 - HER17_RS12250 0.65 -2.8
2,731,979 - HER17_RS12250 0.76 -2.8
2,732,201 - -1.1
2,732,286 + HER17_RS12255 0.19 -3.3
2,732,291 + HER17_RS12255 0.20 -1.3
2,732,291 + HER17_RS12255 0.20 -0.6
2,732,325 - HER17_RS12255 0.24 -3.3

Or see this region's nucleotide sequence