Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS12240 and HER17_RS12245 overlap by 1 nucleotides HER17_RS12245 and HER17_RS12250 are separated by 6 nucleotides HER17_RS12250 and HER17_RS12255 overlap by 19 nucleotides
HER17_RS12240: HER17_RS12240 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,729,756 to 2,730,823
_RS12240
HER17_RS12245: HER17_RS12245 - imidazole glycerol phosphate synthase subunit HisH, at 2,730,823 to 2,731,413
_RS12245
HER17_RS12250: HER17_RS12250 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,731,420 to 2,732,157
_RS12250
HER17_RS12255: HER17_RS12255 - imidazole glycerol phosphate synthase subunit HisF, at 2,732,139 to 2,732,915
_RS12255
Position (kb)
2730
2731
2732 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2729.899 kb on + strand, within HER17_RS12240 at 2730.029 kb on - strand, within HER17_RS12240 at 2730.095 kb on + strand, within HER17_RS12240 at 2730.096 kb on - strand, within HER17_RS12240 at 2730.127 kb on + strand, within HER17_RS12240 at 2730.127 kb on + strand, within HER17_RS12240 at 2730.128 kb on - strand, within HER17_RS12240 at 2730.131 kb on + strand, within HER17_RS12240 at 2730.131 kb on + strand, within HER17_RS12240 at 2730.132 kb on - strand, within HER17_RS12240 at 2730.132 kb on - strand, within HER17_RS12240 at 2730.197 kb on + strand, within HER17_RS12240 at 2730.230 kb on - strand, within HER17_RS12240 at 2730.300 kb on + strand, within HER17_RS12240 at 2730.300 kb on + strand, within HER17_RS12240 at 2730.300 kb on + strand, within HER17_RS12240 at 2730.301 kb on - strand, within HER17_RS12240 at 2730.301 kb on - strand, within HER17_RS12240 at 2730.427 kb on - strand, within HER17_RS12240 at 2730.427 kb on - strand, within HER17_RS12240 at 2730.427 kb on - strand, within HER17_RS12240 at 2730.427 kb on - strand, within HER17_RS12240 at 2730.428 kb on + strand, within HER17_RS12240 at 2730.428 kb on + strand, within HER17_RS12240 at 2730.429 kb on - strand, within HER17_RS12240 at 2730.429 kb on - strand, within HER17_RS12240 at 2730.451 kb on - strand, within HER17_RS12240 at 2730.457 kb on + strand, within HER17_RS12240 at 2730.488 kb on - strand, within HER17_RS12240 at 2730.674 kb on + strand, within HER17_RS12240 at 2730.688 kb on + strand, within HER17_RS12240 at 2730.706 kb on + strand, within HER17_RS12240 at 2730.706 kb on + strand, within HER17_RS12240 at 2730.794 kb on - strand at 2730.875 kb on - strand at 2730.897 kb on + strand, within HER17_RS12245 at 2730.898 kb on - strand, within HER17_RS12245 at 2730.898 kb on - strand, within HER17_RS12245 at 2730.908 kb on + strand, within HER17_RS12245 at 2731.234 kb on + strand, within HER17_RS12245 at 2731.235 kb on - strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.256 kb on - strand, within HER17_RS12245 at 2731.256 kb on - strand, within HER17_RS12245 at 2731.256 kb on - strand, within HER17_RS12245 at 2731.256 kb on - strand, within HER17_RS12245 at 2731.412 kb on - strand at 2731.436 kb on + strand at 2731.436 kb on + strand at 2731.436 kb on + strand at 2731.437 kb on - strand at 2731.613 kb on + strand, within HER17_RS12250 at 2731.694 kb on + strand, within HER17_RS12250 at 2731.694 kb on + strand, within HER17_RS12250 at 2731.694 kb on + strand, within HER17_RS12250 at 2731.695 kb on - strand, within HER17_RS12250 at 2731.898 kb on - strand, within HER17_RS12250 at 2731.898 kb on - strand, within HER17_RS12250 at 2731.898 kb on - strand, within HER17_RS12250 at 2731.979 kb on - strand, within HER17_RS12250 at 2732.201 kb on - strand at 2732.286 kb on + strand, within HER17_RS12255 at 2732.291 kb on + strand, within HER17_RS12255 at 2732.291 kb on + strand, within HER17_RS12255 at 2732.325 kb on - strand, within HER17_RS12255
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,729,899 + HER17_RS12240 0.13 -3.5 2,730,029 - HER17_RS12240 0.26 -3.2 2,730,095 + HER17_RS12240 0.32 -2.7 2,730,096 - HER17_RS12240 0.32 -4.7 2,730,127 + HER17_RS12240 0.35 -3.3 2,730,127 + HER17_RS12240 0.35 -3.3 2,730,128 - HER17_RS12240 0.35 -3.1 2,730,131 + HER17_RS12240 0.35 -2.0 2,730,131 + HER17_RS12240 0.35 -1.9 2,730,132 - HER17_RS12240 0.35 -4.4 2,730,132 - HER17_RS12240 0.35 -2.0 2,730,197 + HER17_RS12240 0.41 -3.1 2,730,230 - HER17_RS12240 0.44 -3.8 2,730,300 + HER17_RS12240 0.51 -2.1 2,730,300 + HER17_RS12240 0.51 -1.7 2,730,300 + HER17_RS12240 0.51 -1.1 2,730,301 - HER17_RS12240 0.51 -2.5 2,730,301 - HER17_RS12240 0.51 -2.6 2,730,427 - HER17_RS12240 0.63 -3.0 2,730,427 - HER17_RS12240 0.63 -2.5 2,730,427 - HER17_RS12240 0.63 -1.8 2,730,427 - HER17_RS12240 0.63 -3.1 2,730,428 + HER17_RS12240 0.63 -4.6 2,730,428 + HER17_RS12240 0.63 -1.6 2,730,429 - HER17_RS12240 0.63 -1.6 2,730,429 - HER17_RS12240 0.63 -2.1 2,730,451 - HER17_RS12240 0.65 -1.7 2,730,457 + HER17_RS12240 0.66 -3.6 2,730,488 - HER17_RS12240 0.69 -4.3 2,730,674 + HER17_RS12240 0.86 -3.7 2,730,688 + HER17_RS12240 0.87 -2.1 2,730,706 + HER17_RS12240 0.89 -1.4 2,730,706 + HER17_RS12240 0.89 -2.8 2,730,794 - -1.1 2,730,875 - -1.7 2,730,897 + HER17_RS12245 0.13 -2.0 2,730,898 - HER17_RS12245 0.13 -2.9 2,730,898 - HER17_RS12245 0.13 -4.6 2,730,908 + HER17_RS12245 0.14 -2.7 2,731,234 + HER17_RS12245 0.70 -1.7 2,731,235 - HER17_RS12245 0.70 -2.7 2,731,255 + HER17_RS12245 0.73 -2.3 2,731,255 + HER17_RS12245 0.73 -1.7 2,731,255 + HER17_RS12245 0.73 -2.5 2,731,255 + HER17_RS12245 0.73 -4.5 2,731,255 + HER17_RS12245 0.73 -0.1 2,731,256 - HER17_RS12245 0.73 -4.5 2,731,256 - HER17_RS12245 0.73 -1.6 2,731,256 - HER17_RS12245 0.73 -2.0 2,731,256 - HER17_RS12245 0.73 -2.9 2,731,412 - -4.9 2,731,436 + -3.2 2,731,436 + -3.7 2,731,436 + -2.0 2,731,437 - -2.0 2,731,613 + HER17_RS12250 0.26 -2.0 2,731,694 + HER17_RS12250 0.37 -4.4 2,731,694 + HER17_RS12250 0.37 -2.1 2,731,694 + HER17_RS12250 0.37 -2.4 2,731,695 - HER17_RS12250 0.37 -3.4 2,731,898 - HER17_RS12250 0.65 -2.6 2,731,898 - HER17_RS12250 0.65 -4.2 2,731,898 - HER17_RS12250 0.65 -2.8 2,731,979 - HER17_RS12250 0.76 -2.8 2,732,201 - -1.1 2,732,286 + HER17_RS12255 0.19 -3.3 2,732,291 + HER17_RS12255 0.20 -1.3 2,732,291 + HER17_RS12255 0.20 -0.6 2,732,325 - HER17_RS12255 0.24 -3.3
Or see this region's nucleotide sequence