Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS17510 and HER17_RS17515 are separated by 67 nucleotides HER17_RS17515 and HER17_RS17520 overlap by 1 nucleotides HER17_RS17520 and HER17_RS17525 are separated by 129 nucleotides HER17_RS17525 and HER17_RS17535 are separated by 291 nucleotides HER17_RS17535 and HER17_RS17540 are separated by 98 nucleotides
HER17_RS17510: HER17_RS17510 - phage/plasmid primase, P4 family, at 3,939,846 to 3,942,173
_RS17510
HER17_RS17515: HER17_RS17515 - DUF1493 family protein, at 3,942,241 to 3,942,570
_RS17515
HER17_RS17520: HER17_RS17520 - hypothetical protein, at 3,942,570 to 3,943,040
_RS17520
HER17_RS17525: HER17_RS17525 - prevent-host-death protein, at 3,943,170 to 3,943,421
_RS17525
HER17_RS17535: HER17_RS17535 - hypothetical protein, at 3,943,713 to 3,943,889
_RS17535
HER17_RS17540: HER17_RS17540 - hypothetical protein, at 3,943,988 to 3,944,521
_RS17540
Position (kb)
3942
3943
3944 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3941.571 kb on + strand, within HER17_RS17510 at 3941.605 kb on + strand, within HER17_RS17510 at 3941.622 kb on - strand, within HER17_RS17510 at 3941.758 kb on + strand, within HER17_RS17510 at 3941.920 kb on - strand, within HER17_RS17510 at 3941.920 kb on - strand, within HER17_RS17510 at 3941.920 kb on - strand, within HER17_RS17510 at 3941.920 kb on - strand, within HER17_RS17510 at 3941.920 kb on - strand, within HER17_RS17510 at 3941.920 kb on - strand, within HER17_RS17510 at 3941.920 kb on - strand, within HER17_RS17510 at 3941.921 kb on + strand, within HER17_RS17510 at 3941.922 kb on - strand, within HER17_RS17510 at 3941.957 kb on + strand at 3941.957 kb on + strand at 3941.958 kb on - strand at 3941.961 kb on + strand at 3941.961 kb on + strand at 3941.962 kb on - strand at 3941.968 kb on - strand at 3941.977 kb on - strand at 3942.035 kb on + strand at 3942.076 kb on - strand at 3942.103 kb on + strand at 3942.103 kb on + strand at 3942.103 kb on + strand at 3942.104 kb on - strand at 3942.104 kb on - strand at 3942.104 kb on - strand at 3942.163 kb on + strand at 3942.183 kb on - strand at 3942.229 kb on + strand at 3942.230 kb on - strand at 3942.232 kb on + strand at 3942.232 kb on + strand at 3942.232 kb on + strand at 3942.232 kb on + strand at 3942.232 kb on + strand at 3942.232 kb on + strand at 3942.232 kb on + strand at 3942.233 kb on - strand at 3942.233 kb on - strand at 3942.248 kb on + strand at 3942.249 kb on - strand at 3942.268 kb on - strand at 3942.279 kb on + strand, within HER17_RS17515 at 3942.347 kb on + strand, within HER17_RS17515 at 3942.390 kb on + strand, within HER17_RS17515 at 3942.390 kb on + strand, within HER17_RS17515 at 3942.403 kb on + strand, within HER17_RS17515 at 3942.447 kb on - strand, within HER17_RS17515 at 3942.457 kb on + strand, within HER17_RS17515 at 3942.483 kb on + strand, within HER17_RS17515 at 3942.484 kb on - strand, within HER17_RS17515 at 3942.495 kb on + strand, within HER17_RS17515 at 3942.495 kb on + strand, within HER17_RS17515 at 3942.530 kb on - strand, within HER17_RS17515 at 3942.530 kb on - strand, within HER17_RS17515 at 3942.578 kb on - strand at 3942.578 kb on - strand at 3942.617 kb on + strand, within HER17_RS17520 at 3942.617 kb on + strand, within HER17_RS17520 at 3942.619 kb on + strand, within HER17_RS17520 at 3942.619 kb on + strand, within HER17_RS17520 at 3942.656 kb on + strand, within HER17_RS17520 at 3942.656 kb on + strand, within HER17_RS17520 at 3942.656 kb on + strand, within HER17_RS17520 at 3942.656 kb on + strand, within HER17_RS17520 at 3942.656 kb on + strand, within HER17_RS17520 at 3942.657 kb on - strand, within HER17_RS17520 at 3942.657 kb on - strand, within HER17_RS17520 at 3942.657 kb on - strand, within HER17_RS17520 at 3942.660 kb on + strand, within HER17_RS17520 at 3942.660 kb on + strand, within HER17_RS17520 at 3942.661 kb on - strand, within HER17_RS17520 at 3942.661 kb on - strand, within HER17_RS17520 at 3942.663 kb on + strand, within HER17_RS17520 at 3942.664 kb on - strand, within HER17_RS17520 at 3942.674 kb on - strand, within HER17_RS17520 at 3942.674 kb on - strand, within HER17_RS17520 at 3942.678 kb on + strand, within HER17_RS17520 at 3942.679 kb on - strand, within HER17_RS17520 at 3942.691 kb on + strand, within HER17_RS17520 at 3942.691 kb on + strand, within HER17_RS17520 at 3942.692 kb on - strand, within HER17_RS17520 at 3942.723 kb on - strand, within HER17_RS17520 at 3942.723 kb on - strand, within HER17_RS17520 at 3942.792 kb on + strand, within HER17_RS17520 at 3942.793 kb on - strand, within HER17_RS17520 at 3942.793 kb on - strand, within HER17_RS17520 at 3942.879 kb on + strand, within HER17_RS17520 at 3942.879 kb on + strand, within HER17_RS17520 at 3942.879 kb on + strand, within HER17_RS17520 at 3942.880 kb on - strand, within HER17_RS17520 at 3942.883 kb on + strand, within HER17_RS17520 at 3942.883 kb on + strand, within HER17_RS17520 at 3942.884 kb on - strand, within HER17_RS17520 at 3942.917 kb on + strand, within HER17_RS17520 at 3942.917 kb on + strand, within HER17_RS17520 at 3942.918 kb on - strand, within HER17_RS17520 at 3942.966 kb on - strand, within HER17_RS17520 at 3942.966 kb on - strand, within HER17_RS17520 at 3942.970 kb on - strand, within HER17_RS17520 at 3943.006 kb on + strand at 3943.007 kb on - strand at 3943.009 kb on + strand at 3943.009 kb on + strand at 3943.010 kb on - strand at 3943.010 kb on - strand at 3943.010 kb on - strand at 3943.010 kb on - strand at 3943.017 kb on - strand at 3943.031 kb on + strand at 3943.035 kb on - strand at 3943.074 kb on + strand at 3943.095 kb on + strand at 3943.095 kb on + strand at 3943.102 kb on + strand at 3943.146 kb on + strand at 3943.147 kb on - strand at 3943.155 kb on + strand at 3943.169 kb on + strand at 3943.169 kb on + strand at 3943.177 kb on + strand at 3943.177 kb on + strand at 3943.209 kb on - strand, within HER17_RS17525 at 3943.303 kb on - strand, within HER17_RS17525 at 3943.303 kb on - strand, within HER17_RS17525 at 3943.303 kb on - strand, within HER17_RS17525 at 3943.418 kb on - strand at 3943.420 kb on - strand at 3943.420 kb on - strand at 3943.420 kb on - strand at 3943.512 kb on + strand at 3943.640 kb on + strand at 3943.719 kb on + strand at 3943.721 kb on + strand at 3943.766 kb on + strand, within HER17_RS17535 at 3943.811 kb on + strand, within HER17_RS17535 at 3943.812 kb on - strand, within HER17_RS17535 at 3943.812 kb on - strand, within HER17_RS17535 at 3943.812 kb on - strand, within HER17_RS17535 at 3943.816 kb on + strand, within HER17_RS17535 at 3943.816 kb on + strand, within HER17_RS17535 at 3943.817 kb on - strand, within HER17_RS17535 at 3943.817 kb on - strand, within HER17_RS17535 at 3943.817 kb on - strand, within HER17_RS17535 at 3943.820 kb on - strand, within HER17_RS17535 at 3943.846 kb on + strand, within HER17_RS17535 at 3943.846 kb on + strand, within HER17_RS17535 at 3943.846 kb on + strand, within HER17_RS17535 at 3943.847 kb on - strand, within HER17_RS17535 at 3943.881 kb on - strand at 3943.899 kb on + strand at 3943.908 kb on + strand at 3943.986 kb on + strand at 3944.025 kb on + strand at 3944.025 kb on + strand at 3944.025 kb on + strand at 3944.026 kb on - strand at 3944.039 kb on + strand at 3944.039 kb on + strand at 3944.039 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,941,571 + HER17_RS17510 0.74 +0.4 3,941,605 + HER17_RS17510 0.76 -0.3 3,941,622 - HER17_RS17510 0.76 -0.9 3,941,758 + HER17_RS17510 0.82 +0.1 3,941,920 - HER17_RS17510 0.89 +1.7 3,941,920 - HER17_RS17510 0.89 -0.9 3,941,920 - HER17_RS17510 0.89 +0.5 3,941,920 - HER17_RS17510 0.89 -0.4 3,941,920 - HER17_RS17510 0.89 -0.2 3,941,920 - HER17_RS17510 0.89 -0.1 3,941,920 - HER17_RS17510 0.89 +1.1 3,941,921 + HER17_RS17510 0.89 +1.1 3,941,922 - HER17_RS17510 0.89 +0.1 3,941,957 + +0.4 3,941,957 + +0.0 3,941,958 - +0.7 3,941,961 + +0.9 3,941,961 + +0.5 3,941,962 - -0.3 3,941,968 - -0.2 3,941,977 - -0.1 3,942,035 + -0.1 3,942,076 - +0.1 3,942,103 + -1.0 3,942,103 + +0.5 3,942,103 + -0.8 3,942,104 - -0.0 3,942,104 - -0.3 3,942,104 - -0.1 3,942,163 + -0.6 3,942,183 - +0.1 3,942,229 + +1.0 3,942,230 - +0.5 3,942,232 + -0.2 3,942,232 + +1.4 3,942,232 + +0.6 3,942,232 + -0.1 3,942,232 + -2.4 3,942,232 + -0.3 3,942,232 + +0.4 3,942,233 - +0.2 3,942,233 - -0.9 3,942,248 + -0.4 3,942,249 - +0.7 3,942,268 - -0.2 3,942,279 + HER17_RS17515 0.12 +0.2 3,942,347 + HER17_RS17515 0.32 +0.1 3,942,390 + HER17_RS17515 0.45 -0.1 3,942,390 + HER17_RS17515 0.45 -0.2 3,942,403 + HER17_RS17515 0.49 +0.0 3,942,447 - HER17_RS17515 0.62 +0.0 3,942,457 + HER17_RS17515 0.65 -0.1 3,942,483 + HER17_RS17515 0.73 -0.7 3,942,484 - HER17_RS17515 0.74 +0.4 3,942,495 + HER17_RS17515 0.77 -1.5 3,942,495 + HER17_RS17515 0.77 +1.2 3,942,530 - HER17_RS17515 0.88 -0.8 3,942,530 - HER17_RS17515 0.88 -0.2 3,942,578 - -0.0 3,942,578 - -0.2 3,942,617 + HER17_RS17520 0.10 -0.3 3,942,617 + HER17_RS17520 0.10 +0.9 3,942,619 + HER17_RS17520 0.10 -0.9 3,942,619 + HER17_RS17520 0.10 +0.3 3,942,656 + HER17_RS17520 0.18 -0.1 3,942,656 + HER17_RS17520 0.18 -0.6 3,942,656 + HER17_RS17520 0.18 -0.0 3,942,656 + HER17_RS17520 0.18 -1.3 3,942,656 + HER17_RS17520 0.18 -0.5 3,942,657 - HER17_RS17520 0.18 -0.3 3,942,657 - HER17_RS17520 0.18 -0.2 3,942,657 - HER17_RS17520 0.18 -0.3 3,942,660 + HER17_RS17520 0.19 -0.2 3,942,660 + HER17_RS17520 0.19 +1.1 3,942,661 - HER17_RS17520 0.19 +0.8 3,942,661 - HER17_RS17520 0.19 +0.2 3,942,663 + HER17_RS17520 0.20 +0.3 3,942,664 - HER17_RS17520 0.20 +0.0 3,942,674 - HER17_RS17520 0.22 +0.7 3,942,674 - HER17_RS17520 0.22 -0.1 3,942,678 + HER17_RS17520 0.23 -0.7 3,942,679 - HER17_RS17520 0.23 +0.4 3,942,691 + HER17_RS17520 0.26 +0.6 3,942,691 + HER17_RS17520 0.26 -0.8 3,942,692 - HER17_RS17520 0.26 +0.8 3,942,723 - HER17_RS17520 0.32 -0.2 3,942,723 - HER17_RS17520 0.32 -0.4 3,942,792 + HER17_RS17520 0.47 +0.7 3,942,793 - HER17_RS17520 0.47 +0.4 3,942,793 - HER17_RS17520 0.47 +0.8 3,942,879 + HER17_RS17520 0.66 +0.1 3,942,879 + HER17_RS17520 0.66 -0.4 3,942,879 + HER17_RS17520 0.66 +0.2 3,942,880 - HER17_RS17520 0.66 -1.0 3,942,883 + HER17_RS17520 0.66 -0.1 3,942,883 + HER17_RS17520 0.66 +1.3 3,942,884 - HER17_RS17520 0.67 +0.3 3,942,917 + HER17_RS17520 0.74 -0.1 3,942,917 + HER17_RS17520 0.74 -1.4 3,942,918 - HER17_RS17520 0.74 -0.0 3,942,966 - HER17_RS17520 0.84 +0.9 3,942,966 - HER17_RS17520 0.84 +1.5 3,942,970 - HER17_RS17520 0.85 +0.7 3,943,006 + -1.1 3,943,007 - +0.5 3,943,009 + -0.4 3,943,009 + +0.3 3,943,010 - -1.5 3,943,010 - +0.5 3,943,010 - -1.7 3,943,010 - +0.4 3,943,017 - -0.2 3,943,031 + +0.1 3,943,035 - +0.0 3,943,074 + +0.1 3,943,095 + +1.1 3,943,095 + +0.3 3,943,102 + -0.0 3,943,146 + -0.1 3,943,147 - -0.4 3,943,155 + -0.5 3,943,169 + +0.3 3,943,169 + +0.1 3,943,177 + -0.5 3,943,177 + +0.4 3,943,209 - HER17_RS17525 0.15 +0.1 3,943,303 - HER17_RS17525 0.53 +0.2 3,943,303 - HER17_RS17525 0.53 +0.3 3,943,303 - HER17_RS17525 0.53 -0.0 3,943,418 - -0.8 3,943,420 - +0.1 3,943,420 - -0.2 3,943,420 - -0.7 3,943,512 + +0.3 3,943,640 + +0.1 3,943,719 + -0.1 3,943,721 + -0.2 3,943,766 + HER17_RS17535 0.30 +0.9 3,943,811 + HER17_RS17535 0.55 +1.1 3,943,812 - HER17_RS17535 0.56 +0.2 3,943,812 - HER17_RS17535 0.56 -0.4 3,943,812 - HER17_RS17535 0.56 +0.4 3,943,816 + HER17_RS17535 0.58 -0.2 3,943,816 + HER17_RS17535 0.58 -0.0 3,943,817 - HER17_RS17535 0.59 +0.6 3,943,817 - HER17_RS17535 0.59 -0.2 3,943,817 - HER17_RS17535 0.59 -0.2 3,943,820 - HER17_RS17535 0.60 +0.5 3,943,846 + HER17_RS17535 0.75 -0.0 3,943,846 + HER17_RS17535 0.75 +0.2 3,943,846 + HER17_RS17535 0.75 +0.0 3,943,847 - HER17_RS17535 0.76 -1.1 3,943,881 - +0.7 3,943,899 + +0.3 3,943,908 + -0.9 3,943,986 + -2.8 3,944,025 + +0.2 3,944,025 + +0.1 3,944,025 + -0.5 3,944,026 - +0.9 3,944,039 + -1.0 3,944,039 + -0.5 3,944,039 + +0.6
Or see this region's nucleotide sequence