Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS21970 and HER17_RS10930 are separated by 7 nucleotides HER17_RS10930 and HER17_RS10935 are separated by 13 nucleotides
HER17_RS21970: HER17_RS21970 - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 2,439,228 to 2,440,888
_RS21970
HER17_RS10930: HER17_RS10930 - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 2,440,896 to 2,442,263
_RS10930
HER17_RS10935: HER17_RS10935 - tryptophan synthase subunit beta, at 2,442,277 to 2,443,470
_RS10935
Position (kb)
2440
2441
2442
2443 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2439.900 kb on + strand, within HER17_RS21970 at 2439.901 kb on - strand, within HER17_RS21970 at 2440.104 kb on - strand, within HER17_RS21970 at 2440.107 kb on + strand, within HER17_RS21970 at 2440.107 kb on + strand, within HER17_RS21970 at 2440.108 kb on - strand, within HER17_RS21970 at 2440.274 kb on - strand, within HER17_RS21970 at 2440.278 kb on + strand, within HER17_RS21970 at 2440.282 kb on + strand, within HER17_RS21970 at 2440.282 kb on + strand, within HER17_RS21970 at 2440.282 kb on + strand, within HER17_RS21970 at 2440.283 kb on - strand, within HER17_RS21970 at 2440.283 kb on - strand, within HER17_RS21970 at 2440.283 kb on - strand, within HER17_RS21970 at 2440.283 kb on - strand, within HER17_RS21970 at 2440.469 kb on + strand, within HER17_RS21970 at 2440.470 kb on - strand, within HER17_RS21970 at 2440.470 kb on - strand, within HER17_RS21970 at 2440.471 kb on + strand, within HER17_RS21970 at 2440.671 kb on + strand, within HER17_RS21970 at 2440.672 kb on - strand, within HER17_RS21970 at 2440.719 kb on + strand, within HER17_RS21970 at 2440.720 kb on - strand, within HER17_RS21970 at 2440.720 kb on - strand, within HER17_RS21970 at 2440.720 kb on - strand, within HER17_RS21970 at 2440.726 kb on + strand at 2440.840 kb on + strand at 2440.840 kb on + strand at 2440.841 kb on - strand at 2440.853 kb on + strand at 2440.886 kb on + strand at 2440.909 kb on + strand at 2440.909 kb on + strand at 2440.910 kb on - strand at 2440.912 kb on + strand at 2440.913 kb on - strand at 2440.913 kb on - strand at 2440.913 kb on - strand at 2440.913 kb on - strand at 2440.916 kb on + strand at 2440.917 kb on - strand at 2440.917 kb on - strand at 2440.917 kb on - strand at 2440.931 kb on + strand at 2441.017 kb on - strand at 2441.036 kb on + strand, within HER17_RS10930 at 2441.036 kb on + strand, within HER17_RS10930 at 2441.048 kb on + strand, within HER17_RS10930 at 2441.130 kb on - strand, within HER17_RS10930 at 2441.172 kb on + strand, within HER17_RS10930 at 2441.219 kb on - strand, within HER17_RS10930 at 2441.249 kb on + strand, within HER17_RS10930 at 2441.250 kb on - strand, within HER17_RS10930 at 2441.268 kb on + strand, within HER17_RS10930 at 2441.283 kb on + strand, within HER17_RS10930 at 2441.284 kb on - strand, within HER17_RS10930 at 2441.335 kb on - strand, within HER17_RS10930 at 2441.371 kb on + strand, within HER17_RS10930 at 2441.407 kb on + strand, within HER17_RS10930 at 2441.712 kb on + strand, within HER17_RS10930 at 2441.712 kb on + strand, within HER17_RS10930 at 2441.713 kb on - strand, within HER17_RS10930 at 2441.766 kb on + strand, within HER17_RS10930 at 2441.767 kb on - strand, within HER17_RS10930 at 2441.767 kb on - strand, within HER17_RS10930 at 2441.817 kb on + strand, within HER17_RS10930 at 2441.876 kb on - strand, within HER17_RS10930 at 2441.876 kb on - strand, within HER17_RS10930 at 2441.876 kb on - strand, within HER17_RS10930 at 2442.024 kb on + strand, within HER17_RS10930 at 2442.025 kb on - strand, within HER17_RS10930 at 2442.056 kb on + strand, within HER17_RS10930 at 2442.057 kb on - strand, within HER17_RS10930 at 2442.132 kb on - strand at 2442.244 kb on + strand at 2442.244 kb on + strand at 2442.244 kb on + strand at 2442.244 kb on + strand at 2442.244 kb on + strand at 2442.244 kb on + strand at 2442.244 kb on + strand at 2442.245 kb on - strand at 2442.245 kb on - strand at 2442.245 kb on - strand at 2442.298 kb on + strand at 2442.463 kb on - strand, within HER17_RS10935 at 2442.496 kb on + strand, within HER17_RS10935 at 2442.497 kb on - strand, within HER17_RS10935 at 2442.674 kb on - strand, within HER17_RS10935 at 2442.674 kb on - strand, within HER17_RS10935 at 2442.676 kb on - strand, within HER17_RS10935 at 2442.818 kb on - strand, within HER17_RS10935 at 2442.832 kb on + strand, within HER17_RS10935 at 2442.833 kb on - strand, within HER17_RS10935 at 2442.833 kb on - strand, within HER17_RS10935 at 2442.833 kb on - strand, within HER17_RS10935 at 2443.018 kb on - strand, within HER17_RS10935 at 2443.111 kb on + strand, within HER17_RS10935 at 2443.111 kb on + strand, within HER17_RS10935 at 2443.112 kb on - strand, within HER17_RS10935 at 2443.219 kb on + strand, within HER17_RS10935 at 2443.219 kb on + strand, within HER17_RS10935 at 2443.220 kb on - strand, within HER17_RS10935
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,439,900 + HER17_RS21970 0.40 -1.8 2,439,901 - HER17_RS21970 0.41 -2.4 2,440,104 - HER17_RS21970 0.53 -3.6 2,440,107 + HER17_RS21970 0.53 -2.9 2,440,107 + HER17_RS21970 0.53 -3.8 2,440,108 - HER17_RS21970 0.53 -3.0 2,440,274 - HER17_RS21970 0.63 -0.6 2,440,278 + HER17_RS21970 0.63 -3.8 2,440,282 + HER17_RS21970 0.63 -4.8 2,440,282 + HER17_RS21970 0.63 -2.0 2,440,282 + HER17_RS21970 0.63 -3.6 2,440,283 - HER17_RS21970 0.64 -1.1 2,440,283 - HER17_RS21970 0.64 -1.3 2,440,283 - HER17_RS21970 0.64 -4.0 2,440,283 - HER17_RS21970 0.64 -2.4 2,440,469 + HER17_RS21970 0.75 -2.6 2,440,470 - HER17_RS21970 0.75 -1.8 2,440,470 - HER17_RS21970 0.75 -3.6 2,440,471 + HER17_RS21970 0.75 -1.4 2,440,671 + HER17_RS21970 0.87 -2.3 2,440,672 - HER17_RS21970 0.87 -2.4 2,440,719 + HER17_RS21970 0.90 -2.0 2,440,720 - HER17_RS21970 0.90 -3.0 2,440,720 - HER17_RS21970 0.90 -3.8 2,440,720 - HER17_RS21970 0.90 -3.3 2,440,726 + -0.3 2,440,840 + -1.9 2,440,840 + -1.3 2,440,841 - -1.5 2,440,853 + -0.6 2,440,886 + -0.4 2,440,909 + -2.9 2,440,909 + -3.3 2,440,910 - -2.0 2,440,912 + -2.1 2,440,913 - -4.3 2,440,913 - -1.6 2,440,913 - -3.1 2,440,913 - -0.3 2,440,916 + -1.5 2,440,917 - -0.9 2,440,917 - -2.4 2,440,917 - -2.9 2,440,931 + -1.5 2,441,017 - -4.5 2,441,036 + HER17_RS10930 0.10 -1.6 2,441,036 + HER17_RS10930 0.10 -3.8 2,441,048 + HER17_RS10930 0.11 -2.1 2,441,130 - HER17_RS10930 0.17 -2.2 2,441,172 + HER17_RS10930 0.20 -3.0 2,441,219 - HER17_RS10930 0.24 -2.7 2,441,249 + HER17_RS10930 0.26 -2.6 2,441,250 - HER17_RS10930 0.26 -2.5 2,441,268 + HER17_RS10930 0.27 -2.4 2,441,283 + HER17_RS10930 0.28 -2.0 2,441,284 - HER17_RS10930 0.28 -3.7 2,441,335 - HER17_RS10930 0.32 -3.5 2,441,371 + HER17_RS10930 0.35 -3.6 2,441,407 + HER17_RS10930 0.37 -3.4 2,441,712 + HER17_RS10930 0.60 -3.1 2,441,712 + HER17_RS10930 0.60 -2.8 2,441,713 - HER17_RS10930 0.60 -2.3 2,441,766 + HER17_RS10930 0.64 -3.5 2,441,767 - HER17_RS10930 0.64 -2.0 2,441,767 - HER17_RS10930 0.64 -2.9 2,441,817 + HER17_RS10930 0.67 -2.4 2,441,876 - HER17_RS10930 0.72 -0.8 2,441,876 - HER17_RS10930 0.72 -2.2 2,441,876 - HER17_RS10930 0.72 -4.1 2,442,024 + HER17_RS10930 0.82 -1.1 2,442,025 - HER17_RS10930 0.83 -3.9 2,442,056 + HER17_RS10930 0.85 -1.2 2,442,057 - HER17_RS10930 0.85 -2.5 2,442,132 - -3.2 2,442,244 + +0.2 2,442,244 + +0.4 2,442,244 + +1.0 2,442,244 + +0.1 2,442,244 + +0.8 2,442,244 + -0.6 2,442,244 + +0.0 2,442,245 - -2.8 2,442,245 - -1.6 2,442,245 - -1.3 2,442,298 + -1.2 2,442,463 - HER17_RS10935 0.16 -2.6 2,442,496 + HER17_RS10935 0.18 -2.6 2,442,497 - HER17_RS10935 0.18 -3.2 2,442,674 - HER17_RS10935 0.33 -3.3 2,442,674 - HER17_RS10935 0.33 -4.4 2,442,676 - HER17_RS10935 0.33 -3.2 2,442,818 - HER17_RS10935 0.45 -2.8 2,442,832 + HER17_RS10935 0.46 -2.9 2,442,833 - HER17_RS10935 0.47 -1.7 2,442,833 - HER17_RS10935 0.47 -4.0 2,442,833 - HER17_RS10935 0.47 -3.0 2,443,018 - HER17_RS10935 0.62 -2.6 2,443,111 + HER17_RS10935 0.70 -2.0 2,443,111 + HER17_RS10935 0.70 -2.6 2,443,112 - HER17_RS10935 0.70 -2.5 2,443,219 + HER17_RS10935 0.79 -3.5 2,443,219 + HER17_RS10935 0.79 -1.3 2,443,220 - HER17_RS10935 0.79 -1.9
Or see this region's nucleotide sequence