Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS00645 and HER17_RS00650 are separated by 10 nucleotides HER17_RS00650 and HER17_RS00655 overlap by 31 nucleotides HER17_RS00655 and HER17_RS00660 are separated by 192 nucleotides
HER17_RS00645: HER17_RS00645 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 148,215 to 148,679
_RS00645
HER17_RS00650: HER17_RS00650 - DUF3142 domain-containing protein, at 148,690 to 149,490
_RS00650
HER17_RS00655: HER17_RS00655 - hypothetical protein, at 149,460 to 151,634
_RS00655
HER17_RS00660: HER17_RS00660 - envelope stress sensor histidine kinase CpxA, at 151,827 to 153,197
_RS00660
Position (kb) 149
150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 148.485 kb on + strand, within HER17_RS00645 at 148.485 kb on + strand, within HER17_RS00645 at 148.486 kb on - strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.501 kb on + strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.502 kb on - strand, within HER17_RS00645 at 148.543 kb on + strand, within HER17_RS00645 at 148.544 kb on - strand, within HER17_RS00645 at 148.544 kb on - strand, within HER17_RS00645 at 148.544 kb on - strand, within HER17_RS00645 at 148.544 kb on - strand, within HER17_RS00645 at 148.544 kb on - strand, within HER17_RS00645 at 148.547 kb on + strand, within HER17_RS00645 at 148.547 kb on + strand, within HER17_RS00645 at 148.595 kb on + strand, within HER17_RS00645 at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.634 kb on + strand at 148.635 kb on - strand at 148.635 kb on - strand at 148.635 kb on - strand at 148.635 kb on - strand at 148.635 kb on - strand at 148.635 kb on - strand at 148.635 kb on - strand at 148.635 kb on - strand at 148.638 kb on + strand at 148.638 kb on + strand at 148.638 kb on + strand at 148.638 kb on + strand at 148.638 kb on + strand at 148.638 kb on + strand at 148.638 kb on + strand at 148.639 kb on - strand at 148.639 kb on - strand at 148.639 kb on - strand at 148.639 kb on - strand at 148.677 kb on + strand at 148.677 kb on + strand at 148.678 kb on - strand at 148.681 kb on + strand at 148.681 kb on + strand at 148.681 kb on + strand at 148.681 kb on + strand at 148.681 kb on + strand at 148.681 kb on + strand at 148.682 kb on - strand at 148.682 kb on - strand at 148.682 kb on - strand at 148.682 kb on - strand at 148.682 kb on - strand at 148.682 kb on - strand at 148.691 kb on + strand at 148.691 kb on + strand at 148.757 kb on + strand at 148.757 kb on + strand at 148.758 kb on - strand at 148.758 kb on - strand at 148.813 kb on + strand, within HER17_RS00650 at 148.859 kb on + strand, within HER17_RS00650 at 148.859 kb on + strand, within HER17_RS00650 at 148.860 kb on - strand, within HER17_RS00650 at 148.868 kb on + strand, within HER17_RS00650 at 148.878 kb on + strand, within HER17_RS00650 at 148.878 kb on + strand, within HER17_RS00650 at 148.879 kb on - strand, within HER17_RS00650 at 148.879 kb on - strand, within HER17_RS00650 at 148.895 kb on + strand, within HER17_RS00650 at 148.984 kb on - strand, within HER17_RS00650 at 148.986 kb on + strand, within HER17_RS00650 at 148.987 kb on - strand, within HER17_RS00650 at 148.987 kb on - strand, within HER17_RS00650 at 149.020 kb on + strand, within HER17_RS00650 at 149.042 kb on + strand, within HER17_RS00650 at 149.042 kb on + strand, within HER17_RS00650 at 149.042 kb on + strand, within HER17_RS00650 at 149.043 kb on - strand, within HER17_RS00650 at 149.131 kb on + strand, within HER17_RS00650 at 149.131 kb on + strand, within HER17_RS00650 at 149.132 kb on - strand, within HER17_RS00650 at 149.132 kb on - strand, within HER17_RS00650 at 149.132 kb on - strand, within HER17_RS00650 at 149.239 kb on + strand, within HER17_RS00650 at 149.247 kb on + strand, within HER17_RS00650 at 149.282 kb on + strand, within HER17_RS00650 at 149.283 kb on - strand, within HER17_RS00650 at 149.421 kb on + strand at 149.468 kb on - strand at 149.470 kb on + strand at 149.504 kb on - strand at 149.590 kb on + strand at 149.591 kb on - strand at 149.609 kb on - strand at 149.618 kb on - strand at 149.618 kb on - strand at 149.618 kb on - strand at 149.665 kb on + strand at 149.665 kb on + strand at 149.665 kb on + strand at 149.666 kb on - strand at 149.666 kb on - strand at 149.666 kb on - strand at 149.666 kb on - strand at 149.666 kb on - strand at 149.732 kb on + strand, within HER17_RS00655 at 149.732 kb on + strand, within HER17_RS00655 at 149.829 kb on + strand, within HER17_RS00655 at 149.829 kb on + strand, within HER17_RS00655 at 149.867 kb on - strand, within HER17_RS00655 at 149.867 kb on - strand, within HER17_RS00655 at 149.867 kb on - strand, within HER17_RS00655 at 149.957 kb on + strand, within HER17_RS00655 at 149.958 kb on - strand, within HER17_RS00655 at 149.958 kb on - strand, within HER17_RS00655 at 149.958 kb on - strand, within HER17_RS00655 at 149.958 kb on - strand, within HER17_RS00655 at 149.987 kb on - strand, within HER17_RS00655 at 150.029 kb on + strand, within HER17_RS00655 at 150.029 kb on + strand, within HER17_RS00655 at 150.030 kb on - strand, within HER17_RS00655 at 150.030 kb on - strand, within HER17_RS00655 at 150.104 kb on + strand, within HER17_RS00655 at 150.336 kb on + strand, within HER17_RS00655 at 150.337 kb on - strand, within HER17_RS00655 at 150.409 kb on + strand, within HER17_RS00655 at 150.409 kb on + strand, within HER17_RS00655 at 150.410 kb on - strand, within HER17_RS00655 at 150.410 kb on - strand, within HER17_RS00655 at 150.414 kb on + strand, within HER17_RS00655 at 150.414 kb on + strand, within HER17_RS00655 at 150.415 kb on - strand, within HER17_RS00655 at 150.415 kb on - strand, within HER17_RS00655 at 150.415 kb on - strand, within HER17_RS00655 at 150.433 kb on + strand, within HER17_RS00655 at 150.433 kb on + strand, within HER17_RS00655 at 150.433 kb on + strand, within HER17_RS00655 at 150.434 kb on - strand, within HER17_RS00655 at 150.434 kb on - strand, within HER17_RS00655 at 150.434 kb on - strand, within HER17_RS00655 at 150.722 kb on - strand, within HER17_RS00655 at 150.757 kb on + strand, within HER17_RS00655 at 150.757 kb on + strand, within HER17_RS00655 at 150.757 kb on + strand, within HER17_RS00655 at 150.758 kb on - strand, within HER17_RS00655 at 150.758 kb on - strand, within HER17_RS00655 at 150.758 kb on - strand, within HER17_RS00655 at 150.758 kb on - strand, within HER17_RS00655 at 150.784 kb on + strand, within HER17_RS00655 at 150.784 kb on + strand, within HER17_RS00655 at 150.785 kb on - strand, within HER17_RS00655 at 150.794 kb on + strand, within HER17_RS00655 at 150.795 kb on - strand, within HER17_RS00655 at 150.833 kb on + strand, within HER17_RS00655 at 150.854 kb on - strand, within HER17_RS00655 at 150.905 kb on + strand, within HER17_RS00655 at 150.905 kb on + strand, within HER17_RS00655 at 150.905 kb on + strand, within HER17_RS00655 at 150.906 kb on - strand, within HER17_RS00655 at 150.906 kb on - strand, within HER17_RS00655 at 150.906 kb on - strand, within HER17_RS00655 at 150.906 kb on - strand, within HER17_RS00655 at 150.906 kb on - strand, within HER17_RS00655 at 150.907 kb on + strand, within HER17_RS00655 at 150.907 kb on + strand, within HER17_RS00655 at 150.907 kb on + strand, within HER17_RS00655 at 150.907 kb on + strand, within HER17_RS00655 at 150.907 kb on + strand, within HER17_RS00655 at 150.908 kb on - strand, within HER17_RS00655 at 150.908 kb on - strand, within HER17_RS00655 at 150.908 kb on - strand, within HER17_RS00655 at 150.908 kb on - strand, within HER17_RS00655 at 151.015 kb on + strand, within HER17_RS00655 at 151.015 kb on + strand, within HER17_RS00655 at 151.016 kb on - strand, within HER17_RS00655 at 151.016 kb on - strand, within HER17_RS00655 at 151.016 kb on - strand, within HER17_RS00655 at 151.050 kb on + strand, within HER17_RS00655 at 151.056 kb on + strand, within HER17_RS00655 at 151.056 kb on + strand, within HER17_RS00655 at 151.056 kb on + strand, within HER17_RS00655 at 151.056 kb on + strand, within HER17_RS00655 at 151.057 kb on - strand, within HER17_RS00655 at 151.057 kb on - strand, within HER17_RS00655 at 151.057 kb on - strand, within HER17_RS00655 at 151.057 kb on - strand, within HER17_RS00655 at 151.057 kb on - strand, within HER17_RS00655 at 151.057 kb on - strand, within HER17_RS00655 at 151.085 kb on + strand, within HER17_RS00655 at 151.086 kb on - strand, within HER17_RS00655 at 151.086 kb on - strand, within HER17_RS00655 at 151.086 kb on - strand, within HER17_RS00655 at 151.087 kb on + strand, within HER17_RS00655 at 151.088 kb on - strand, within HER17_RS00655 at 151.209 kb on - strand, within HER17_RS00655 at 151.247 kb on + strand, within HER17_RS00655 at 151.248 kb on - strand, within HER17_RS00655 at 151.485 kb on - strand at 151.513 kb on + strand at 151.514 kb on - strand at 151.641 kb on + strand at 151.641 kb on + strand at 151.642 kb on - strand at 151.696 kb on - strand at 151.724 kb on + strand at 151.726 kb on + strand at 151.726 kb on + strand at 151.727 kb on - strand at 151.727 kb on - strand at 151.740 kb on + strand at 151.741 kb on - strand at 151.786 kb on + strand at 151.788 kb on + strand at 151.788 kb on + strand at 151.913 kb on + strand at 151.913 kb on + strand at 152.107 kb on + strand, within HER17_RS00660 at 152.244 kb on + strand, within HER17_RS00660 at 152.384 kb on + strand, within HER17_RS00660 at 152.583 kb on + strand, within HER17_RS00660 at 152.584 kb on - strand, within HER17_RS00660 at 152.584 kb on - strand, within HER17_RS00660
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 148,485 + HER17_RS00645 0.58 -0.6 148,485 + HER17_RS00645 0.58 -0.8 148,486 - HER17_RS00645 0.58 -0.1 148,501 + HER17_RS00645 0.62 -0.0 148,501 + HER17_RS00645 0.62 -1.9 148,501 + HER17_RS00645 0.62 -1.1 148,501 + HER17_RS00645 0.62 +0.0 148,501 + HER17_RS00645 0.62 +1.1 148,501 + HER17_RS00645 0.62 -1.3 148,501 + HER17_RS00645 0.62 +0.1 148,501 + HER17_RS00645 0.62 -0.2 148,501 + HER17_RS00645 0.62 +0.5 148,501 + HER17_RS00645 0.62 -0.1 148,501 + HER17_RS00645 0.62 -0.5 148,501 + HER17_RS00645 0.62 +0.5 148,501 + HER17_RS00645 0.62 -0.2 148,501 + HER17_RS00645 0.62 +0.4 148,501 + HER17_RS00645 0.62 +0.9 148,502 - HER17_RS00645 0.62 -0.5 148,502 - HER17_RS00645 0.62 +1.2 148,502 - HER17_RS00645 0.62 -1.7 148,502 - HER17_RS00645 0.62 +0.2 148,502 - HER17_RS00645 0.62 -0.3 148,502 - HER17_RS00645 0.62 +0.1 148,502 - HER17_RS00645 0.62 -0.0 148,502 - HER17_RS00645 0.62 +0.0 148,502 - HER17_RS00645 0.62 -0.4 148,502 - HER17_RS00645 0.62 +0.5 148,543 + HER17_RS00645 0.71 -0.9 148,544 - HER17_RS00645 0.71 +0.8 148,544 - HER17_RS00645 0.71 +0.6 148,544 - HER17_RS00645 0.71 +0.1 148,544 - HER17_RS00645 0.71 +0.1 148,544 - HER17_RS00645 0.71 -1.0 148,547 + HER17_RS00645 0.71 -0.3 148,547 + HER17_RS00645 0.71 +0.0 148,595 + HER17_RS00645 0.82 +0.5 148,634 + +0.2 148,634 + -0.7 148,634 + -0.1 148,634 + +0.3 148,634 + +1.5 148,634 + -1.0 148,634 + +0.4 148,634 + +0.2 148,634 + +0.8 148,634 + +0.2 148,634 + -0.2 148,635 - -0.8 148,635 - +0.1 148,635 - +0.5 148,635 - -0.4 148,635 - -0.5 148,635 - +0.3 148,635 - -0.6 148,635 - +0.1 148,638 + -1.2 148,638 + -0.2 148,638 + -0.9 148,638 + +0.2 148,638 + +0.0 148,638 + +0.7 148,638 + +0.4 148,639 - +0.1 148,639 - -0.6 148,639 - +0.4 148,639 - -0.2 148,677 + +0.7 148,677 + -0.4 148,678 - +0.5 148,681 + +0.9 148,681 + -0.3 148,681 + -0.3 148,681 + -0.2 148,681 + +0.4 148,681 + +0.1 148,682 - -0.9 148,682 - +0.0 148,682 - -1.1 148,682 - +0.7 148,682 - +0.5 148,682 - +0.1 148,691 + -0.5 148,691 + -0.1 148,757 + -0.7 148,757 + -1.4 148,758 - +0.7 148,758 - +0.9 148,813 + HER17_RS00650 0.15 +1.3 148,859 + HER17_RS00650 0.21 +0.9 148,859 + HER17_RS00650 0.21 -0.2 148,860 - HER17_RS00650 0.21 +0.0 148,868 + HER17_RS00650 0.22 -0.1 148,878 + HER17_RS00650 0.23 +0.4 148,878 + HER17_RS00650 0.23 -0.6 148,879 - HER17_RS00650 0.24 +0.0 148,879 - HER17_RS00650 0.24 -0.0 148,895 + HER17_RS00650 0.26 +0.4 148,984 - HER17_RS00650 0.37 +0.2 148,986 + HER17_RS00650 0.37 -0.3 148,987 - HER17_RS00650 0.37 +0.3 148,987 - HER17_RS00650 0.37 +1.0 149,020 + HER17_RS00650 0.41 -0.3 149,042 + HER17_RS00650 0.44 +0.3 149,042 + HER17_RS00650 0.44 +0.7 149,042 + HER17_RS00650 0.44 +0.2 149,043 - HER17_RS00650 0.44 -0.2 149,131 + HER17_RS00650 0.55 +0.4 149,131 + HER17_RS00650 0.55 +0.2 149,132 - HER17_RS00650 0.55 +0.3 149,132 - HER17_RS00650 0.55 -3.0 149,132 - HER17_RS00650 0.55 +0.4 149,239 + HER17_RS00650 0.69 +0.3 149,247 + HER17_RS00650 0.70 -0.3 149,282 + HER17_RS00650 0.74 -1.6 149,283 - HER17_RS00650 0.74 +0.5 149,421 + +0.9 149,468 - +1.4 149,470 + +0.1 149,504 - +0.4 149,590 + +0.4 149,591 - -0.5 149,609 - -0.2 149,618 - +0.8 149,618 - +0.3 149,618 - -1.1 149,665 + -0.7 149,665 + -0.8 149,665 + -0.2 149,666 - -0.1 149,666 - -0.1 149,666 - +0.1 149,666 - +0.2 149,666 - -0.1 149,732 + HER17_RS00655 0.13 -0.1 149,732 + HER17_RS00655 0.13 +0.1 149,829 + HER17_RS00655 0.17 +0.8 149,829 + HER17_RS00655 0.17 +1.0 149,867 - HER17_RS00655 0.19 +1.5 149,867 - HER17_RS00655 0.19 -0.6 149,867 - HER17_RS00655 0.19 -1.7 149,957 + HER17_RS00655 0.23 +0.6 149,958 - HER17_RS00655 0.23 +0.4 149,958 - HER17_RS00655 0.23 -0.5 149,958 - HER17_RS00655 0.23 -0.2 149,958 - HER17_RS00655 0.23 +0.8 149,987 - HER17_RS00655 0.24 -0.3 150,029 + HER17_RS00655 0.26 -0.3 150,029 + HER17_RS00655 0.26 +0.6 150,030 - HER17_RS00655 0.26 -0.1 150,030 - HER17_RS00655 0.26 -0.4 150,104 + HER17_RS00655 0.30 +0.4 150,336 + HER17_RS00655 0.40 +0.2 150,337 - HER17_RS00655 0.40 +0.2 150,409 + HER17_RS00655 0.44 +0.7 150,409 + HER17_RS00655 0.44 +0.5 150,410 - HER17_RS00655 0.44 -1.5 150,410 - HER17_RS00655 0.44 -0.6 150,414 + HER17_RS00655 0.44 -0.9 150,414 + HER17_RS00655 0.44 -1.0 150,415 - HER17_RS00655 0.44 -0.7 150,415 - HER17_RS00655 0.44 -0.0 150,415 - HER17_RS00655 0.44 -0.3 150,433 + HER17_RS00655 0.45 -0.8 150,433 + HER17_RS00655 0.45 -0.1 150,433 + HER17_RS00655 0.45 -0.0 150,434 - HER17_RS00655 0.45 -0.3 150,434 - HER17_RS00655 0.45 +0.1 150,434 - HER17_RS00655 0.45 +0.3 150,722 - HER17_RS00655 0.58 +0.8 150,757 + HER17_RS00655 0.60 +0.1 150,757 + HER17_RS00655 0.60 -0.1 150,757 + HER17_RS00655 0.60 +0.3 150,758 - HER17_RS00655 0.60 +1.0 150,758 - HER17_RS00655 0.60 +0.4 150,758 - HER17_RS00655 0.60 +0.1 150,758 - HER17_RS00655 0.60 -0.5 150,784 + HER17_RS00655 0.61 -0.5 150,784 + HER17_RS00655 0.61 +0.1 150,785 - HER17_RS00655 0.61 -1.1 150,794 + HER17_RS00655 0.61 -0.6 150,795 - HER17_RS00655 0.61 -0.6 150,833 + HER17_RS00655 0.63 -0.3 150,854 - HER17_RS00655 0.64 -1.3 150,905 + HER17_RS00655 0.66 -0.1 150,905 + HER17_RS00655 0.66 -0.2 150,905 + HER17_RS00655 0.66 +0.9 150,906 - HER17_RS00655 0.66 +0.3 150,906 - HER17_RS00655 0.66 +0.4 150,906 - HER17_RS00655 0.66 -1.3 150,906 - HER17_RS00655 0.66 -1.1 150,906 - HER17_RS00655 0.66 -0.3 150,907 + HER17_RS00655 0.67 -0.2 150,907 + HER17_RS00655 0.67 -1.4 150,907 + HER17_RS00655 0.67 +1.5 150,907 + HER17_RS00655 0.67 +0.0 150,907 + HER17_RS00655 0.67 -0.1 150,908 - HER17_RS00655 0.67 +0.2 150,908 - HER17_RS00655 0.67 -0.4 150,908 - HER17_RS00655 0.67 +0.2 150,908 - HER17_RS00655 0.67 -0.0 151,015 + HER17_RS00655 0.71 +0.1 151,015 + HER17_RS00655 0.71 +0.7 151,016 - HER17_RS00655 0.72 +0.2 151,016 - HER17_RS00655 0.72 +1.4 151,016 - HER17_RS00655 0.72 -0.7 151,050 + HER17_RS00655 0.73 -0.8 151,056 + HER17_RS00655 0.73 +1.0 151,056 + HER17_RS00655 0.73 +0.4 151,056 + HER17_RS00655 0.73 -0.2 151,056 + HER17_RS00655 0.73 +1.0 151,057 - HER17_RS00655 0.73 +0.2 151,057 - HER17_RS00655 0.73 +0.2 151,057 - HER17_RS00655 0.73 +0.4 151,057 - HER17_RS00655 0.73 -1.1 151,057 - HER17_RS00655 0.73 +0.3 151,057 - HER17_RS00655 0.73 +3.1 151,085 + HER17_RS00655 0.75 +0.3 151,086 - HER17_RS00655 0.75 -0.7 151,086 - HER17_RS00655 0.75 -1.7 151,086 - HER17_RS00655 0.75 +0.2 151,087 + HER17_RS00655 0.75 -0.5 151,088 - HER17_RS00655 0.75 +0.2 151,209 - HER17_RS00655 0.80 +1.0 151,247 + HER17_RS00655 0.82 -0.6 151,248 - HER17_RS00655 0.82 -0.4 151,485 - +0.7 151,513 + +0.4 151,514 - -0.0 151,641 + -0.6 151,641 + +0.3 151,642 - +0.7 151,696 - +0.2 151,724 + +0.3 151,726 + +0.0 151,726 + +0.1 151,727 - +1.1 151,727 - -0.0 151,740 + -0.5 151,741 - +0.1 151,786 + -0.3 151,788 + +0.4 151,788 + -0.5 151,913 + -0.7 151,913 + -0.9 152,107 + HER17_RS00660 0.20 +3.1 152,244 + HER17_RS00660 0.30 +0.7 152,384 + HER17_RS00660 0.41 -0.9 152,583 + HER17_RS00660 0.55 -1.8 152,584 - HER17_RS00660 0.55 +1.6 152,584 - HER17_RS00660 0.55 +0.5
Or see this region's nucleotide sequence