Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS12545 and HER17_RS12550 are separated by 287 nucleotides HER17_RS12550 and HER17_RS12555 overlap by 8 nucleotides HER17_RS12555 and HER17_RS12560 are separated by 81 nucleotides
HER17_RS12545: HER17_RS12545 - serine hydrolase, at 2,801,857 to 2,803,062
_RS12545
HER17_RS12550: HER17_RS12550 - ABC transporter permease, at 2,803,350 to 2,804,009
_RS12550
HER17_RS12555: HER17_RS12555 - methionine ABC transporter ATP-binding protein, at 2,804,002 to 2,805,018
_RS12555
HER17_RS12560: HER17_RS12560 - MetQ/NlpA family ABC transporter substrate-binding protein, at 2,805,100 to 2,805,945
_RS12560
Position (kb)
2804
2805
2806 Strain fitness (log2 ratio)
-1
0
1 at 2803.014 kb on + strand at 2803.014 kb on + strand at 2803.019 kb on - strand at 2803.019 kb on - strand at 2803.019 kb on - strand at 2803.134 kb on + strand at 2803.134 kb on + strand at 2803.134 kb on + strand at 2803.134 kb on + strand at 2803.135 kb on - strand at 2803.135 kb on - strand at 2803.135 kb on - strand at 2803.135 kb on - strand at 2803.346 kb on - strand at 2803.346 kb on - strand at 2803.469 kb on - strand, within HER17_RS12550 at 2803.616 kb on + strand, within HER17_RS12550 at 2803.616 kb on + strand, within HER17_RS12550 at 2803.617 kb on - strand, within HER17_RS12550 at 2803.702 kb on + strand, within HER17_RS12550 at 2803.702 kb on + strand, within HER17_RS12550 at 2803.757 kb on + strand, within HER17_RS12550 at 2803.817 kb on + strand, within HER17_RS12550 at 2803.829 kb on + strand, within HER17_RS12550 at 2803.829 kb on + strand, within HER17_RS12550 at 2803.829 kb on + strand, within HER17_RS12550 at 2803.830 kb on - strand, within HER17_RS12550 at 2803.830 kb on - strand, within HER17_RS12550 at 2803.855 kb on + strand, within HER17_RS12550 at 2803.938 kb on + strand, within HER17_RS12550 at 2803.938 kb on + strand, within HER17_RS12550 at 2803.939 kb on - strand, within HER17_RS12550 at 2803.998 kb on + strand at 2803.999 kb on - strand at 2804.246 kb on - strand, within HER17_RS12555 at 2804.307 kb on + strand, within HER17_RS12555 at 2804.440 kb on - strand, within HER17_RS12555 at 2804.565 kb on + strand, within HER17_RS12555 at 2804.566 kb on - strand, within HER17_RS12555 at 2804.566 kb on - strand, within HER17_RS12555 at 2804.570 kb on - strand, within HER17_RS12555 at 2804.755 kb on + strand, within HER17_RS12555 at 2804.756 kb on - strand, within HER17_RS12555 at 2804.756 kb on - strand, within HER17_RS12555 at 2804.999 kb on - strand at 2805.007 kb on + strand at 2805.008 kb on - strand at 2805.064 kb on + strand at 2805.065 kb on - strand at 2805.081 kb on - strand at 2805.088 kb on - strand at 2805.090 kb on - strand at 2805.102 kb on - strand at 2805.158 kb on + strand at 2805.158 kb on + strand at 2805.158 kb on + strand at 2805.191 kb on + strand, within HER17_RS12560 at 2805.192 kb on - strand, within HER17_RS12560 at 2805.192 kb on - strand, within HER17_RS12560 at 2805.219 kb on + strand, within HER17_RS12560 at 2805.265 kb on - strand, within HER17_RS12560 at 2805.265 kb on - strand, within HER17_RS12560 at 2805.268 kb on - strand, within HER17_RS12560 at 2805.439 kb on - strand, within HER17_RS12560 at 2805.439 kb on - strand, within HER17_RS12560 at 2805.541 kb on - strand, within HER17_RS12560 at 2805.677 kb on + strand, within HER17_RS12560 at 2805.684 kb on + strand, within HER17_RS12560 at 2805.684 kb on + strand, within HER17_RS12560 at 2805.684 kb on + strand, within HER17_RS12560 at 2805.684 kb on + strand, within HER17_RS12560 at 2805.684 kb on + strand, within HER17_RS12560 at 2805.684 kb on + strand, within HER17_RS12560 at 2805.685 kb on - strand, within HER17_RS12560 at 2805.685 kb on - strand, within HER17_RS12560 at 2805.685 kb on - strand, within HER17_RS12560 at 2805.708 kb on + strand, within HER17_RS12560 at 2805.708 kb on + strand, within HER17_RS12560 at 2805.743 kb on + strand, within HER17_RS12560 at 2805.743 kb on + strand, within HER17_RS12560 at 2805.743 kb on + strand, within HER17_RS12560 at 2805.744 kb on - strand, within HER17_RS12560 at 2805.970 kb on - strand at 2805.986 kb on + strand at 2805.987 kb on - strand at 2806.001 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,803,014 + +1.1 2,803,014 + -0.1 2,803,019 - +1.1 2,803,019 - +0.2 2,803,019 - -0.3 2,803,134 + +0.7 2,803,134 + -0.2 2,803,134 + -0.3 2,803,134 + +0.6 2,803,135 - -0.7 2,803,135 - +1.3 2,803,135 - +0.0 2,803,135 - +0.3 2,803,346 - -0.6 2,803,346 - +0.3 2,803,469 - HER17_RS12550 0.18 -0.1 2,803,616 + HER17_RS12550 0.40 -0.2 2,803,616 + HER17_RS12550 0.40 -0.1 2,803,617 - HER17_RS12550 0.40 +0.1 2,803,702 + HER17_RS12550 0.53 -0.3 2,803,702 + HER17_RS12550 0.53 +0.3 2,803,757 + HER17_RS12550 0.62 +1.1 2,803,817 + HER17_RS12550 0.71 +0.4 2,803,829 + HER17_RS12550 0.73 +0.7 2,803,829 + HER17_RS12550 0.73 -1.0 2,803,829 + HER17_RS12550 0.73 +0.2 2,803,830 - HER17_RS12550 0.73 -0.3 2,803,830 - HER17_RS12550 0.73 -0.0 2,803,855 + HER17_RS12550 0.77 -1.1 2,803,938 + HER17_RS12550 0.89 +0.1 2,803,938 + HER17_RS12550 0.89 +0.2 2,803,939 - HER17_RS12550 0.89 +0.3 2,803,998 + +0.2 2,803,999 - +0.2 2,804,246 - HER17_RS12555 0.24 -0.1 2,804,307 + HER17_RS12555 0.30 -0.2 2,804,440 - HER17_RS12555 0.43 -0.6 2,804,565 + HER17_RS12555 0.55 +0.6 2,804,566 - HER17_RS12555 0.55 +0.4 2,804,566 - HER17_RS12555 0.55 -0.4 2,804,570 - HER17_RS12555 0.56 +0.2 2,804,755 + HER17_RS12555 0.74 +0.2 2,804,756 - HER17_RS12555 0.74 +0.1 2,804,756 - HER17_RS12555 0.74 +0.1 2,804,999 - +0.1 2,805,007 + -0.6 2,805,008 - +0.1 2,805,064 + +0.6 2,805,065 - +1.0 2,805,081 - +0.0 2,805,088 - -0.4 2,805,090 - +0.2 2,805,102 - -0.6 2,805,158 + +0.5 2,805,158 + +1.0 2,805,158 + -0.4 2,805,191 + HER17_RS12560 0.11 -0.2 2,805,192 - HER17_RS12560 0.11 -0.8 2,805,192 - HER17_RS12560 0.11 +0.1 2,805,219 + HER17_RS12560 0.14 +0.0 2,805,265 - HER17_RS12560 0.20 -0.5 2,805,265 - HER17_RS12560 0.20 +0.1 2,805,268 - HER17_RS12560 0.20 -0.3 2,805,439 - HER17_RS12560 0.40 +0.3 2,805,439 - HER17_RS12560 0.40 +1.0 2,805,541 - HER17_RS12560 0.52 -0.1 2,805,677 + HER17_RS12560 0.68 +0.4 2,805,684 + HER17_RS12560 0.69 +0.4 2,805,684 + HER17_RS12560 0.69 -0.1 2,805,684 + HER17_RS12560 0.69 +0.0 2,805,684 + HER17_RS12560 0.69 +0.5 2,805,684 + HER17_RS12560 0.69 +0.3 2,805,684 + HER17_RS12560 0.69 +0.1 2,805,685 - HER17_RS12560 0.69 +1.2 2,805,685 - HER17_RS12560 0.69 -0.3 2,805,685 - HER17_RS12560 0.69 +0.4 2,805,708 + HER17_RS12560 0.72 +0.3 2,805,708 + HER17_RS12560 0.72 +0.3 2,805,743 + HER17_RS12560 0.76 +0.2 2,805,743 + HER17_RS12560 0.76 +0.2 2,805,743 + HER17_RS12560 0.76 -0.7 2,805,744 - HER17_RS12560 0.76 -0.8 2,805,970 - -1.4 2,805,986 + +0.6 2,805,987 - -0.9 2,806,001 - +0.6
Or see this region's nucleotide sequence