Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS02300 and HER17_RS02305 are separated by 272 nucleotides HER17_RS02305 and HER17_RS02310 are separated by 316 nucleotides
HER17_RS02300: HER17_RS02300 - bifunctional isocitrate dehydrogenase kinase/phosphatase, at 510,096 to 511,871
_RS02300
HER17_RS02305: HER17_RS02305 - glyoxylate bypass operon transcriptional repressor IclR, at 512,144 to 512,974
_RS02305
HER17_RS02310: HER17_RS02310 - methionine synthase, at 513,291 to 516,974
_RS02310
Position (kb)
512
513 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 511.164 kb on + strand, within HER17_RS02300 at 511.165 kb on - strand, within HER17_RS02300 at 511.165 kb on - strand, within HER17_RS02300 at 511.165 kb on - strand, within HER17_RS02300 at 511.219 kb on - strand, within HER17_RS02300 at 511.335 kb on + strand, within HER17_RS02300 at 511.335 kb on + strand, within HER17_RS02300 at 511.335 kb on + strand, within HER17_RS02300 at 511.335 kb on + strand, within HER17_RS02300 at 511.336 kb on - strand, within HER17_RS02300 at 511.336 kb on - strand, within HER17_RS02300 at 511.336 kb on - strand, within HER17_RS02300 at 511.337 kb on + strand, within HER17_RS02300 at 511.368 kb on + strand, within HER17_RS02300 at 511.369 kb on - strand, within HER17_RS02300 at 511.369 kb on - strand, within HER17_RS02300 at 511.471 kb on + strand, within HER17_RS02300 at 511.472 kb on - strand, within HER17_RS02300 at 511.515 kb on + strand, within HER17_RS02300 at 511.515 kb on + strand, within HER17_RS02300 at 511.516 kb on - strand, within HER17_RS02300 at 511.516 kb on - strand, within HER17_RS02300 at 511.521 kb on + strand, within HER17_RS02300 at 511.521 kb on + strand, within HER17_RS02300 at 511.521 kb on + strand, within HER17_RS02300 at 511.522 kb on - strand, within HER17_RS02300 at 511.522 kb on - strand, within HER17_RS02300 at 511.536 kb on + strand, within HER17_RS02300 at 511.536 kb on + strand, within HER17_RS02300 at 511.536 kb on + strand, within HER17_RS02300 at 511.536 kb on + strand, within HER17_RS02300 at 511.537 kb on - strand, within HER17_RS02300 at 511.537 kb on - strand, within HER17_RS02300 at 511.537 kb on - strand, within HER17_RS02300 at 511.550 kb on + strand, within HER17_RS02300 at 511.595 kb on - strand, within HER17_RS02300 at 511.611 kb on + strand, within HER17_RS02300 at 511.612 kb on - strand, within HER17_RS02300 at 511.726 kb on - strand at 511.738 kb on + strand at 511.749 kb on - strand at 511.776 kb on + strand at 511.777 kb on - strand at 511.777 kb on - strand at 511.777 kb on - strand at 511.824 kb on + strand at 511.824 kb on + strand at 511.824 kb on + strand at 511.824 kb on + strand at 511.825 kb on - strand at 511.825 kb on - strand at 511.825 kb on - strand at 511.825 kb on - strand at 511.829 kb on + strand at 511.829 kb on + strand at 511.829 kb on + strand at 511.829 kb on + strand at 511.829 kb on + strand at 511.829 kb on + strand at 511.832 kb on + strand at 511.832 kb on + strand at 511.833 kb on - strand at 511.833 kb on - strand at 512.001 kb on + strand at 512.024 kb on - strand at 512.068 kb on - strand at 512.084 kb on + strand at 512.085 kb on - strand at 512.090 kb on + strand at 512.091 kb on - strand at 512.091 kb on - strand at 512.094 kb on - strand at 512.094 kb on - strand at 512.094 kb on - strand at 512.094 kb on - strand at 512.124 kb on + strand at 512.125 kb on - strand at 512.287 kb on + strand, within HER17_RS02305 at 512.287 kb on + strand, within HER17_RS02305 at 512.407 kb on + strand, within HER17_RS02305 at 512.408 kb on - strand, within HER17_RS02305 at 512.408 kb on - strand, within HER17_RS02305 at 512.408 kb on - strand, within HER17_RS02305 at 512.459 kb on + strand, within HER17_RS02305 at 512.459 kb on + strand, within HER17_RS02305 at 512.459 kb on + strand, within HER17_RS02305 at 512.459 kb on + strand, within HER17_RS02305 at 512.545 kb on + strand, within HER17_RS02305 at 512.545 kb on + strand, within HER17_RS02305 at 512.545 kb on + strand, within HER17_RS02305 at 512.545 kb on + strand, within HER17_RS02305 at 512.578 kb on + strand, within HER17_RS02305 at 512.579 kb on - strand, within HER17_RS02305 at 512.579 kb on - strand, within HER17_RS02305 at 512.631 kb on - strand, within HER17_RS02305 at 512.639 kb on + strand, within HER17_RS02305 at 512.639 kb on + strand, within HER17_RS02305 at 512.640 kb on - strand, within HER17_RS02305 at 512.640 kb on - strand, within HER17_RS02305 at 512.640 kb on - strand, within HER17_RS02305 at 512.640 kb on - strand, within HER17_RS02305 at 512.857 kb on + strand, within HER17_RS02305 at 512.858 kb on - strand, within HER17_RS02305 at 512.859 kb on + strand, within HER17_RS02305 at 512.860 kb on - strand, within HER17_RS02305 at 512.860 kb on - strand, within HER17_RS02305 at 512.860 kb on - strand, within HER17_RS02305 at 512.860 kb on - strand, within HER17_RS02305 at 512.860 kb on - strand, within HER17_RS02305 at 512.860 kb on - strand, within HER17_RS02305 at 512.992 kb on - strand at 513.021 kb on - strand at 513.087 kb on + strand at 513.113 kb on + strand at 513.113 kb on + strand at 513.113 kb on + strand at 513.113 kb on + strand at 513.113 kb on + strand at 513.114 kb on - strand at 513.114 kb on - strand at 513.114 kb on - strand at 513.114 kb on - strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.118 kb on + strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.119 kb on - strand at 513.126 kb on - strand at 513.126 kb on - strand at 513.126 kb on - strand at 513.145 kb on - strand at 513.145 kb on - strand at 513.145 kb on - strand at 513.145 kb on - strand at 513.145 kb on - strand at 513.145 kb on - strand at 513.145 kb on - strand at 513.182 kb on + strand at 513.182 kb on + strand at 513.182 kb on + strand at 513.182 kb on + strand at 513.182 kb on + strand at 513.182 kb on + strand at 513.248 kb on + strand at 513.249 kb on - strand at 513.255 kb on + strand at 513.255 kb on + strand at 513.256 kb on - strand at 513.259 kb on + strand at 513.259 kb on + strand at 513.259 kb on + strand at 513.259 kb on + strand at 513.260 kb on - strand at 513.260 kb on - strand at 513.260 kb on - strand at 513.264 kb on + strand at 513.265 kb on - strand at 513.265 kb on - strand at 513.265 kb on - strand at 513.269 kb on - strand at 513.338 kb on + strand at 513.338 kb on + strand at 513.339 kb on - strand at 513.356 kb on + strand at 513.447 kb on - strand at 513.447 kb on - strand at 513.457 kb on + strand at 513.570 kb on + strand at 513.575 kb on + strand at 513.576 kb on - strand at 513.662 kb on + strand, within HER17_RS02310 at 513.672 kb on + strand, within HER17_RS02310 at 513.858 kb on - strand, within HER17_RS02310 at 513.954 kb on - strand, within HER17_RS02310
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 511,164 + HER17_RS02300 0.60 +0.5 511,165 - HER17_RS02300 0.60 +0.1 511,165 - HER17_RS02300 0.60 +0.2 511,165 - HER17_RS02300 0.60 -0.0 511,219 - HER17_RS02300 0.63 +0.2 511,335 + HER17_RS02300 0.70 -0.1 511,335 + HER17_RS02300 0.70 +0.2 511,335 + HER17_RS02300 0.70 -1.3 511,335 + HER17_RS02300 0.70 -0.3 511,336 - HER17_RS02300 0.70 +1.3 511,336 - HER17_RS02300 0.70 +0.6 511,336 - HER17_RS02300 0.70 +1.5 511,337 + HER17_RS02300 0.70 +1.0 511,368 + HER17_RS02300 0.72 -0.9 511,369 - HER17_RS02300 0.72 +0.4 511,369 - HER17_RS02300 0.72 -1.2 511,471 + HER17_RS02300 0.77 +0.2 511,472 - HER17_RS02300 0.77 +0.7 511,515 + HER17_RS02300 0.80 -0.3 511,515 + HER17_RS02300 0.80 -0.7 511,516 - HER17_RS02300 0.80 +0.9 511,516 - HER17_RS02300 0.80 +0.2 511,521 + HER17_RS02300 0.80 +0.5 511,521 + HER17_RS02300 0.80 +0.0 511,521 + HER17_RS02300 0.80 +0.3 511,522 - HER17_RS02300 0.80 -0.6 511,522 - HER17_RS02300 0.80 +0.3 511,536 + HER17_RS02300 0.81 -0.0 511,536 + HER17_RS02300 0.81 -0.3 511,536 + HER17_RS02300 0.81 -0.1 511,536 + HER17_RS02300 0.81 -0.5 511,537 - HER17_RS02300 0.81 -0.8 511,537 - HER17_RS02300 0.81 -0.1 511,537 - HER17_RS02300 0.81 -1.1 511,550 + HER17_RS02300 0.82 +0.2 511,595 - HER17_RS02300 0.84 +2.1 511,611 + HER17_RS02300 0.85 -0.5 511,612 - HER17_RS02300 0.85 -0.3 511,726 - -0.5 511,738 + -0.5 511,749 - -0.4 511,776 + -0.3 511,777 - +1.2 511,777 - +0.3 511,777 - +0.2 511,824 + +0.2 511,824 + +0.2 511,824 + +0.4 511,824 + -0.5 511,825 - +0.6 511,825 - +0.5 511,825 - -0.1 511,825 - +1.3 511,829 + -0.5 511,829 + +0.3 511,829 + -0.5 511,829 + +0.8 511,829 + +0.0 511,829 + -0.3 511,832 + +0.4 511,832 + +0.5 511,833 - +0.2 511,833 - -0.1 512,001 + +0.2 512,024 - -0.8 512,068 - +0.9 512,084 + +0.2 512,085 - +0.5 512,090 + +1.1 512,091 - +0.3 512,091 - -0.2 512,094 - -0.5 512,094 - -0.6 512,094 - -0.4 512,094 - -0.2 512,124 + +0.1 512,125 - +0.3 512,287 + HER17_RS02305 0.17 -0.2 512,287 + HER17_RS02305 0.17 +0.1 512,407 + HER17_RS02305 0.32 +0.9 512,408 - HER17_RS02305 0.32 +0.5 512,408 - HER17_RS02305 0.32 +1.2 512,408 - HER17_RS02305 0.32 +0.1 512,459 + HER17_RS02305 0.38 +0.4 512,459 + HER17_RS02305 0.38 -0.7 512,459 + HER17_RS02305 0.38 -1.8 512,459 + HER17_RS02305 0.38 -0.7 512,545 + HER17_RS02305 0.48 +0.1 512,545 + HER17_RS02305 0.48 +0.6 512,545 + HER17_RS02305 0.48 +1.2 512,545 + HER17_RS02305 0.48 +0.1 512,578 + HER17_RS02305 0.52 +0.8 512,579 - HER17_RS02305 0.52 -0.1 512,579 - HER17_RS02305 0.52 -0.3 512,631 - HER17_RS02305 0.59 -0.2 512,639 + HER17_RS02305 0.60 +0.4 512,639 + HER17_RS02305 0.60 +1.5 512,640 - HER17_RS02305 0.60 -1.0 512,640 - HER17_RS02305 0.60 -0.6 512,640 - HER17_RS02305 0.60 +0.3 512,640 - HER17_RS02305 0.60 +0.6 512,857 + HER17_RS02305 0.86 -0.4 512,858 - HER17_RS02305 0.86 +0.0 512,859 + HER17_RS02305 0.86 +0.3 512,860 - HER17_RS02305 0.86 -0.1 512,860 - HER17_RS02305 0.86 -0.7 512,860 - HER17_RS02305 0.86 -0.2 512,860 - HER17_RS02305 0.86 +0.1 512,860 - HER17_RS02305 0.86 -1.3 512,860 - HER17_RS02305 0.86 +0.0 512,992 - +0.3 513,021 - +0.2 513,087 + +0.7 513,113 + +0.3 513,113 + +0.7 513,113 + -0.8 513,113 + -0.4 513,113 + +0.5 513,114 - +0.1 513,114 - +0.0 513,114 - -1.8 513,114 - +0.0 513,118 + +0.0 513,118 + +0.7 513,118 + -3.3 513,118 + +0.0 513,118 + -0.0 513,119 - +1.0 513,119 - +0.1 513,119 - +0.5 513,119 - +0.0 513,119 - +0.8 513,126 - -0.2 513,126 - +0.2 513,126 - +0.8 513,145 - +0.2 513,145 - +0.1 513,145 - +2.0 513,145 - +0.7 513,145 - +0.7 513,145 - +0.4 513,145 - -1.3 513,182 + -0.3 513,182 + +0.6 513,182 + +0.4 513,182 + -0.0 513,182 + +0.2 513,182 + -1.5 513,248 + -0.2 513,249 - +0.5 513,255 + +1.0 513,255 + -0.3 513,256 - +0.3 513,259 + -0.2 513,259 + -0.5 513,259 + +0.3 513,259 + -0.5 513,260 - -0.7 513,260 - -0.4 513,260 - +0.4 513,264 + +0.9 513,265 - +0.4 513,265 - -0.2 513,265 - +0.1 513,269 - -0.2 513,338 + +0.4 513,338 + -0.2 513,339 - +0.2 513,356 + +0.2 513,447 - -0.1 513,447 - +0.5 513,457 + -0.0 513,570 + -0.5 513,575 + -1.1 513,576 - +0.7 513,662 + HER17_RS02310 0.10 -0.8 513,672 + HER17_RS02310 0.10 +0.1 513,858 - HER17_RS02310 0.15 -1.9 513,954 - HER17_RS02310 0.18 -0.5
Or see this region's nucleotide sequence