Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS20730 and HER17_RS20735 are separated by 142 nucleotides HER17_RS20735 and HER17_RS20740 overlap by 1 nucleotides HER17_RS20740 and HER17_RS20745 are separated by 219 nucleotides
HER17_RS20730: HER17_RS20730 - serine O-acetyltransferase, at 4,647,202 to 4,648,023
_RS20730
HER17_RS20735: HER17_RS20735 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 4,648,166 to 4,649,185
_RS20735
HER17_RS20740: HER17_RS20740 - protein-export chaperone SecB, at 4,649,185 to 4,649,655
_RS20740
HER17_RS20745: HER17_RS20745 - rhodanese-like domain-containing protein, at 4,649,875 to 4,650,306
_RS20745
Position (kb)
4648
4649
4650 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4647.169 kb on + strand at 4647.170 kb on - strand at 4647.170 kb on - strand at 4647.170 kb on - strand at 4647.170 kb on - strand at 4647.203 kb on + strand at 4647.204 kb on - strand at 4647.204 kb on - strand at 4647.204 kb on - strand at 4647.204 kb on - strand at 4647.208 kb on - strand at 4647.249 kb on - strand at 4647.249 kb on - strand at 4647.249 kb on - strand at 4647.297 kb on + strand, within HER17_RS20730 at 4647.297 kb on + strand, within HER17_RS20730 at 4647.298 kb on - strand, within HER17_RS20730 at 4647.298 kb on - strand, within HER17_RS20730 at 4647.678 kb on + strand, within HER17_RS20730 at 4647.731 kb on + strand, within HER17_RS20730 at 4647.732 kb on - strand, within HER17_RS20730 at 4647.871 kb on - strand, within HER17_RS20730 at 4647.884 kb on + strand, within HER17_RS20730 at 4647.885 kb on - strand, within HER17_RS20730 at 4647.885 kb on - strand, within HER17_RS20730 at 4647.901 kb on + strand, within HER17_RS20730 at 4647.901 kb on + strand, within HER17_RS20730 at 4648.030 kb on - strand at 4648.030 kb on - strand at 4648.042 kb on - strand at 4648.058 kb on + strand at 4648.058 kb on + strand at 4648.059 kb on - strand at 4648.059 kb on - strand at 4648.059 kb on - strand at 4648.059 kb on - strand at 4648.059 kb on - strand at 4648.059 kb on - strand at 4648.059 kb on - strand at 4648.060 kb on + strand at 4648.060 kb on + strand at 4648.061 kb on - strand at 4648.061 kb on - strand at 4648.061 kb on - strand at 4648.061 kb on - strand at 4648.102 kb on - strand at 4648.102 kb on - strand at 4648.103 kb on + strand at 4648.104 kb on - strand at 4648.104 kb on - strand at 4648.119 kb on + strand at 4648.119 kb on + strand at 4648.120 kb on - strand at 4648.120 kb on - strand at 4648.120 kb on - strand at 4648.122 kb on + strand at 4648.122 kb on + strand at 4648.122 kb on + strand at 4648.122 kb on + strand at 4648.122 kb on + strand at 4648.122 kb on + strand at 4648.122 kb on + strand at 4648.122 kb on + strand at 4648.122 kb on + strand at 4648.123 kb on - strand at 4648.123 kb on - strand at 4648.123 kb on - strand at 4648.123 kb on - strand at 4648.123 kb on - strand at 4648.123 kb on - strand at 4648.123 kb on - strand at 4648.123 kb on - strand at 4648.152 kb on + strand at 4648.153 kb on - strand at 4648.153 kb on - strand at 4648.153 kb on - strand at 4648.167 kb on + strand at 4648.205 kb on + strand at 4648.206 kb on - strand at 4648.206 kb on - strand at 4648.242 kb on + strand at 4648.242 kb on + strand at 4648.243 kb on - strand at 4648.243 kb on - strand at 4648.243 kb on - strand at 4648.243 kb on - strand at 4648.243 kb on - strand at 4648.299 kb on - strand, within HER17_RS20735 at 4648.475 kb on - strand, within HER17_RS20735 at 4648.540 kb on + strand, within HER17_RS20735 at 4648.647 kb on + strand, within HER17_RS20735 at 4648.775 kb on + strand, within HER17_RS20735 at 4648.793 kb on - strand, within HER17_RS20735 at 4648.998 kb on - strand, within HER17_RS20735 at 4649.292 kb on + strand, within HER17_RS20740 at 4649.331 kb on - strand, within HER17_RS20740 at 4649.489 kb on + strand, within HER17_RS20740 at 4649.489 kb on + strand, within HER17_RS20740 at 4649.489 kb on + strand, within HER17_RS20740 at 4649.490 kb on - strand, within HER17_RS20740 at 4649.490 kb on - strand, within HER17_RS20740 at 4649.490 kb on - strand, within HER17_RS20740 at 4649.606 kb on + strand, within HER17_RS20740 at 4649.606 kb on + strand, within HER17_RS20740 at 4649.610 kb on + strand at 4649.659 kb on - strand at 4649.659 kb on - strand at 4649.662 kb on + strand at 4649.662 kb on + strand at 4649.662 kb on + strand at 4649.663 kb on - strand at 4649.663 kb on - strand at 4649.666 kb on - strand at 4649.666 kb on - strand at 4649.666 kb on - strand at 4649.666 kb on - strand at 4649.677 kb on - strand at 4649.704 kb on + strand at 4649.704 kb on + strand at 4649.705 kb on - strand at 4649.705 kb on - strand at 4649.755 kb on + strand at 4649.774 kb on - strand at 4649.826 kb on + strand at 4649.826 kb on + strand at 4649.826 kb on + strand at 4649.826 kb on + strand at 4649.827 kb on - strand at 4649.827 kb on - strand at 4649.827 kb on - strand at 4649.827 kb on - strand at 4649.835 kb on + strand at 4649.835 kb on + strand at 4649.835 kb on + strand at 4649.835 kb on + strand at 4649.836 kb on - strand at 4649.836 kb on - strand at 4649.836 kb on - strand at 4649.861 kb on + strand at 4649.876 kb on + strand at 4649.936 kb on - strand, within HER17_RS20745 at 4650.030 kb on + strand, within HER17_RS20745 at 4650.030 kb on + strand, within HER17_RS20745 at 4650.031 kb on - strand, within HER17_RS20745 at 4650.049 kb on + strand, within HER17_RS20745 at 4650.049 kb on + strand, within HER17_RS20745 at 4650.049 kb on + strand, within HER17_RS20745 at 4650.049 kb on + strand, within HER17_RS20745 at 4650.049 kb on + strand, within HER17_RS20745 at 4650.049 kb on + strand, within HER17_RS20745 at 4650.050 kb on - strand, within HER17_RS20745 at 4650.100 kb on - strand, within HER17_RS20745 at 4650.157 kb on - strand, within HER17_RS20745
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,647,169 + +0.0 4,647,170 - -0.7 4,647,170 - -0.5 4,647,170 - -0.1 4,647,170 - -0.9 4,647,203 + +0.3 4,647,204 - +0.7 4,647,204 - -0.7 4,647,204 - +0.1 4,647,204 - +1.1 4,647,208 - +0.1 4,647,249 - -1.4 4,647,249 - -0.7 4,647,249 - +0.2 4,647,297 + HER17_RS20730 0.12 -0.1 4,647,297 + HER17_RS20730 0.12 -0.4 4,647,298 - HER17_RS20730 0.12 -2.2 4,647,298 - HER17_RS20730 0.12 -0.3 4,647,678 + HER17_RS20730 0.58 -0.3 4,647,731 + HER17_RS20730 0.64 -0.3 4,647,732 - HER17_RS20730 0.64 -0.0 4,647,871 - HER17_RS20730 0.81 -0.6 4,647,884 + HER17_RS20730 0.83 +0.7 4,647,885 - HER17_RS20730 0.83 -0.9 4,647,885 - HER17_RS20730 0.83 +0.4 4,647,901 + HER17_RS20730 0.85 -0.1 4,647,901 + HER17_RS20730 0.85 +0.2 4,648,030 - -0.1 4,648,030 - -0.1 4,648,042 - -0.5 4,648,058 + -1.5 4,648,058 + +0.4 4,648,059 - +0.8 4,648,059 - -0.3 4,648,059 - -0.9 4,648,059 - -0.1 4,648,059 - -0.1 4,648,059 - -1.5 4,648,059 - +0.4 4,648,060 + +0.5 4,648,060 + +0.2 4,648,061 - -0.4 4,648,061 - +0.3 4,648,061 - +0.4 4,648,061 - -0.3 4,648,102 - +0.3 4,648,102 - +0.6 4,648,103 + -0.1 4,648,104 - -0.2 4,648,104 - +0.3 4,648,119 + -0.1 4,648,119 + -0.5 4,648,120 - -0.8 4,648,120 - +0.1 4,648,120 - -0.1 4,648,122 + -1.3 4,648,122 + +1.0 4,648,122 + -1.1 4,648,122 + -0.2 4,648,122 + -0.5 4,648,122 + -0.2 4,648,122 + -0.0 4,648,122 + -0.8 4,648,122 + +0.5 4,648,123 - -3.0 4,648,123 - -0.0 4,648,123 - +1.5 4,648,123 - -0.4 4,648,123 - +0.7 4,648,123 - +0.8 4,648,123 - +0.1 4,648,123 - -0.8 4,648,152 + -0.6 4,648,153 - +0.4 4,648,153 - +0.1 4,648,153 - +0.2 4,648,167 + -1.1 4,648,205 + -2.1 4,648,206 - -1.5 4,648,206 - -3.4 4,648,242 + -1.3 4,648,242 + -4.0 4,648,243 - -0.0 4,648,243 - -3.5 4,648,243 - -2.3 4,648,243 - -1.8 4,648,243 - -2.7 4,648,299 - HER17_RS20735 0.13 -2.4 4,648,475 - HER17_RS20735 0.30 -0.8 4,648,540 + HER17_RS20735 0.37 -2.8 4,648,647 + HER17_RS20735 0.47 -3.4 4,648,775 + HER17_RS20735 0.60 -5.0 4,648,793 - HER17_RS20735 0.61 -3.1 4,648,998 - HER17_RS20735 0.82 -2.1 4,649,292 + HER17_RS20740 0.23 +0.4 4,649,331 - HER17_RS20740 0.31 -1.0 4,649,489 + HER17_RS20740 0.65 -1.3 4,649,489 + HER17_RS20740 0.65 -2.2 4,649,489 + HER17_RS20740 0.65 -2.5 4,649,490 - HER17_RS20740 0.65 -0.8 4,649,490 - HER17_RS20740 0.65 -1.8 4,649,490 - HER17_RS20740 0.65 -0.9 4,649,606 + HER17_RS20740 0.89 -1.3 4,649,606 + HER17_RS20740 0.89 -1.7 4,649,610 + -0.9 4,649,659 - -1.5 4,649,659 - -1.0 4,649,662 + -2.8 4,649,662 + -2.0 4,649,662 + -0.5 4,649,663 - -1.0 4,649,663 - -0.8 4,649,666 - -2.3 4,649,666 - +1.0 4,649,666 - -1.0 4,649,666 - -1.7 4,649,677 - -0.1 4,649,704 + -1.7 4,649,704 + -2.0 4,649,705 - -1.2 4,649,705 - -0.0 4,649,755 + -3.0 4,649,774 - +0.9 4,649,826 + +0.2 4,649,826 + +1.0 4,649,826 + -0.5 4,649,826 + +0.6 4,649,827 - -0.2 4,649,827 - +0.6 4,649,827 - +0.0 4,649,827 - +0.4 4,649,835 + -1.7 4,649,835 + -0.7 4,649,835 + -0.8 4,649,835 + -0.7 4,649,836 - +0.1 4,649,836 - +0.8 4,649,836 - +0.3 4,649,861 + -1.5 4,649,876 + -1.3 4,649,936 - HER17_RS20745 0.14 -1.0 4,650,030 + HER17_RS20745 0.36 -0.0 4,650,030 + HER17_RS20745 0.36 -0.9 4,650,031 - HER17_RS20745 0.36 -0.1 4,650,049 + HER17_RS20745 0.40 +0.1 4,650,049 + HER17_RS20745 0.40 +0.0 4,650,049 + HER17_RS20745 0.40 +0.5 4,650,049 + HER17_RS20745 0.40 -0.4 4,650,049 + HER17_RS20745 0.40 -0.4 4,650,049 + HER17_RS20745 0.40 +0.5 4,650,050 - HER17_RS20745 0.41 -0.4 4,650,100 - HER17_RS20745 0.52 -0.2 4,650,157 - HER17_RS20745 0.65 -0.3
Or see this region's nucleotide sequence