Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS15935 and HER17_RS15940 are separated by 68 nucleotides HER17_RS15940 and HER17_RS15945 overlap by 26 nucleotides HER17_RS15945 and HER17_RS15950 are separated by 68 nucleotides HER17_RS15950 and HER17_RS15955 are separated by 24 nucleotides
HER17_RS15935: HER17_RS15935 - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,578,973 to 3,580,838
_RS15935
HER17_RS15940: HER17_RS15940 - phosphatidylglycerophosphatase A, at 3,580,907 to 3,581,443
_RS15940
HER17_RS15945: HER17_RS15945 - thiamine-phosphate kinase, at 3,581,418 to 3,582,398
_RS15945
HER17_RS15950: HER17_RS15950 - transcription antitermination factor NusB, at 3,582,467 to 3,582,886
_RS15950
HER17_RS15955: HER17_RS15955 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,582,911 to 3,583,387
_RS15955
Position (kb)
3581
3582
3583 Strain fitness (log2 ratio)
-2
-1
0
1 at 3580.837 kb on - strand at 3580.841 kb on + strand at 3580.842 kb on - strand at 3580.842 kb on - strand at 3580.842 kb on - strand at 3580.878 kb on - strand at 3580.939 kb on - strand at 3580.942 kb on + strand at 3580.943 kb on - strand at 3580.984 kb on + strand, within HER17_RS15940 at 3580.985 kb on - strand, within HER17_RS15940 at 3581.093 kb on - strand, within HER17_RS15940 at 3581.200 kb on - strand, within HER17_RS15940 at 3581.210 kb on - strand, within HER17_RS15940 at 3581.433 kb on - strand at 3581.443 kb on + strand at 3581.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,580,837 - -0.6 3,580,841 + +0.2 3,580,842 - +0.3 3,580,842 - -1.3 3,580,842 - +0.1 3,580,878 - +0.3 3,580,939 - -0.0 3,580,942 + +0.4 3,580,943 - -2.7 3,580,984 + HER17_RS15940 0.14 -2.6 3,580,985 - HER17_RS15940 0.15 -0.9 3,581,093 - HER17_RS15940 0.35 -1.1 3,581,200 - HER17_RS15940 0.55 -0.8 3,581,210 - HER17_RS15940 0.56 -0.5 3,581,433 - +0.9 3,581,443 + +0.2 3,581,444 - -0.7
Or see this region's nucleotide sequence