Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS12240 and HER17_RS12245 overlap by 1 nucleotides HER17_RS12245 and HER17_RS12250 are separated by 6 nucleotides HER17_RS12250 and HER17_RS12255 overlap by 19 nucleotides
HER17_RS12240: HER17_RS12240 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,729,756 to 2,730,823
_RS12240
HER17_RS12245: HER17_RS12245 - imidazole glycerol phosphate synthase subunit HisH, at 2,730,823 to 2,731,413
_RS12245
HER17_RS12250: HER17_RS12250 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,731,420 to 2,732,157
_RS12250
HER17_RS12255: HER17_RS12255 - imidazole glycerol phosphate synthase subunit HisF, at 2,732,139 to 2,732,915
_RS12255
Position (kb)
2730
2731
2732 Strain fitness (log2 ratio)
-1
0
1 at 2729.899 kb on + strand, within HER17_RS12240 at 2730.029 kb on - strand, within HER17_RS12240 at 2730.095 kb on + strand, within HER17_RS12240 at 2730.096 kb on - strand, within HER17_RS12240 at 2730.127 kb on + strand, within HER17_RS12240 at 2730.127 kb on + strand, within HER17_RS12240 at 2730.128 kb on - strand, within HER17_RS12240 at 2730.131 kb on + strand, within HER17_RS12240 at 2730.131 kb on + strand, within HER17_RS12240 at 2730.132 kb on - strand, within HER17_RS12240 at 2730.132 kb on - strand, within HER17_RS12240 at 2730.197 kb on + strand, within HER17_RS12240 at 2730.230 kb on - strand, within HER17_RS12240 at 2730.300 kb on + strand, within HER17_RS12240 at 2730.300 kb on + strand, within HER17_RS12240 at 2730.300 kb on + strand, within HER17_RS12240 at 2730.301 kb on - strand, within HER17_RS12240 at 2730.301 kb on - strand, within HER17_RS12240 at 2730.427 kb on - strand, within HER17_RS12240 at 2730.427 kb on - strand, within HER17_RS12240 at 2730.427 kb on - strand, within HER17_RS12240 at 2730.427 kb on - strand, within HER17_RS12240 at 2730.428 kb on + strand, within HER17_RS12240 at 2730.428 kb on + strand, within HER17_RS12240 at 2730.429 kb on - strand, within HER17_RS12240 at 2730.429 kb on - strand, within HER17_RS12240 at 2730.451 kb on - strand, within HER17_RS12240 at 2730.457 kb on + strand, within HER17_RS12240 at 2730.488 kb on - strand, within HER17_RS12240 at 2730.674 kb on + strand, within HER17_RS12240 at 2730.688 kb on + strand, within HER17_RS12240 at 2730.706 kb on + strand, within HER17_RS12240 at 2730.706 kb on + strand, within HER17_RS12240 at 2730.794 kb on - strand at 2730.875 kb on - strand at 2730.897 kb on + strand, within HER17_RS12245 at 2730.898 kb on - strand, within HER17_RS12245 at 2730.898 kb on - strand, within HER17_RS12245 at 2730.908 kb on + strand, within HER17_RS12245 at 2731.234 kb on + strand, within HER17_RS12245 at 2731.235 kb on - strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.255 kb on + strand, within HER17_RS12245 at 2731.256 kb on - strand, within HER17_RS12245 at 2731.256 kb on - strand, within HER17_RS12245 at 2731.256 kb on - strand, within HER17_RS12245 at 2731.256 kb on - strand, within HER17_RS12245 at 2731.412 kb on - strand at 2731.436 kb on + strand at 2731.436 kb on + strand at 2731.436 kb on + strand at 2731.437 kb on - strand at 2731.613 kb on + strand, within HER17_RS12250 at 2731.694 kb on + strand, within HER17_RS12250 at 2731.694 kb on + strand, within HER17_RS12250 at 2731.694 kb on + strand, within HER17_RS12250 at 2731.695 kb on - strand, within HER17_RS12250 at 2731.898 kb on - strand, within HER17_RS12250 at 2731.898 kb on - strand, within HER17_RS12250 at 2731.898 kb on - strand, within HER17_RS12250 at 2731.979 kb on - strand, within HER17_RS12250 at 2732.201 kb on - strand at 2732.286 kb on + strand, within HER17_RS12255 at 2732.291 kb on + strand, within HER17_RS12255 at 2732.291 kb on + strand, within HER17_RS12255 at 2732.325 kb on - strand, within HER17_RS12255
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,729,899 + HER17_RS12240 0.13 -0.3 2,730,029 - HER17_RS12240 0.26 -0.1 2,730,095 + HER17_RS12240 0.32 -0.4 2,730,096 - HER17_RS12240 0.32 -0.4 2,730,127 + HER17_RS12240 0.35 -1.0 2,730,127 + HER17_RS12240 0.35 +0.3 2,730,128 - HER17_RS12240 0.35 +0.1 2,730,131 + HER17_RS12240 0.35 +0.5 2,730,131 + HER17_RS12240 0.35 +0.4 2,730,132 - HER17_RS12240 0.35 -0.1 2,730,132 - HER17_RS12240 0.35 +0.0 2,730,197 + HER17_RS12240 0.41 -0.8 2,730,230 - HER17_RS12240 0.44 -0.8 2,730,300 + HER17_RS12240 0.51 -0.4 2,730,300 + HER17_RS12240 0.51 +1.2 2,730,300 + HER17_RS12240 0.51 -0.1 2,730,301 - HER17_RS12240 0.51 -1.3 2,730,301 - HER17_RS12240 0.51 -0.2 2,730,427 - HER17_RS12240 0.63 -1.3 2,730,427 - HER17_RS12240 0.63 +0.1 2,730,427 - HER17_RS12240 0.63 -0.1 2,730,427 - HER17_RS12240 0.63 -0.3 2,730,428 + HER17_RS12240 0.63 +0.1 2,730,428 + HER17_RS12240 0.63 +1.0 2,730,429 - HER17_RS12240 0.63 -0.2 2,730,429 - HER17_RS12240 0.63 -1.1 2,730,451 - HER17_RS12240 0.65 +1.0 2,730,457 + HER17_RS12240 0.66 -0.7 2,730,488 - HER17_RS12240 0.69 -1.1 2,730,674 + HER17_RS12240 0.86 -0.2 2,730,688 + HER17_RS12240 0.87 +0.2 2,730,706 + HER17_RS12240 0.89 +0.7 2,730,706 + HER17_RS12240 0.89 +0.2 2,730,794 - +0.9 2,730,875 - -0.3 2,730,897 + HER17_RS12245 0.13 +0.5 2,730,898 - HER17_RS12245 0.13 -0.7 2,730,898 - HER17_RS12245 0.13 -0.1 2,730,908 + HER17_RS12245 0.14 +0.0 2,731,234 + HER17_RS12245 0.70 -0.4 2,731,235 - HER17_RS12245 0.70 -0.7 2,731,255 + HER17_RS12245 0.73 -0.5 2,731,255 + HER17_RS12245 0.73 -0.4 2,731,255 + HER17_RS12245 0.73 +0.8 2,731,255 + HER17_RS12245 0.73 +0.2 2,731,255 + HER17_RS12245 0.73 +1.6 2,731,256 - HER17_RS12245 0.73 +0.0 2,731,256 - HER17_RS12245 0.73 -0.2 2,731,256 - HER17_RS12245 0.73 +0.3 2,731,256 - HER17_RS12245 0.73 +0.7 2,731,412 - -0.5 2,731,436 + +0.7 2,731,436 + -1.1 2,731,436 + +0.0 2,731,437 - -0.7 2,731,613 + HER17_RS12250 0.26 +0.5 2,731,694 + HER17_RS12250 0.37 +0.2 2,731,694 + HER17_RS12250 0.37 +0.4 2,731,694 + HER17_RS12250 0.37 -1.0 2,731,695 - HER17_RS12250 0.37 -0.7 2,731,898 - HER17_RS12250 0.65 +0.7 2,731,898 - HER17_RS12250 0.65 +0.0 2,731,898 - HER17_RS12250 0.65 -0.1 2,731,979 - HER17_RS12250 0.76 +1.0 2,732,201 - +1.6 2,732,286 + HER17_RS12255 0.19 +0.6 2,732,291 + HER17_RS12255 0.20 +0.3 2,732,291 + HER17_RS12255 0.20 +0.7 2,732,325 - HER17_RS12255 0.24 -0.6
Or see this region's nucleotide sequence