Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS02335 and HER17_RS02340 are separated by 509 nucleotides HER17_RS02340 and HER17_RS02345 are separated by 43 nucleotides HER17_RS02345 and HER17_RS02350 are separated by 60 nucleotides
HER17_RS02335: HER17_RS02335 - lysine-sensitive aspartokinase 3, at 524,581 to 525,957
_RS02335
HER17_RS02340: HER17_RS02340 - glucose-6-phosphate isomerase, at 526,467 to 528,116
_RS02340
HER17_RS02345: HER17_RS02345 - DUF4156 domain-containing protein, at 528,160 to 528,504
_RS02345
HER17_RS02350: HER17_RS02350 - EF-P beta-lysylation protein EpmB, at 528,565 to 529,617
_RS02350
Position (kb) 526
527
528
529 Strain fitness (log2 ratio) -2
-1
0
1
2 at 525.564 kb on + strand, within HER17_RS02335 at 525.716 kb on + strand, within HER17_RS02335 at 525.941 kb on - strand at 525.996 kb on + strand at 526.011 kb on + strand at 526.011 kb on + strand at 526.067 kb on + strand at 526.067 kb on + strand at 526.072 kb on + strand at 526.072 kb on + strand at 526.073 kb on - strand at 526.073 kb on - strand at 526.073 kb on - strand at 526.114 kb on + strand at 526.169 kb on + strand at 526.237 kb on + strand at 526.237 kb on + strand at 526.237 kb on + strand at 526.237 kb on + strand at 526.237 kb on + strand at 526.238 kb on - strand at 526.238 kb on - strand at 526.238 kb on - strand at 526.283 kb on + strand at 526.291 kb on + strand at 526.297 kb on + strand at 526.306 kb on - strand at 526.306 kb on - strand at 526.381 kb on - strand at 526.417 kb on + strand at 526.417 kb on + strand at 526.418 kb on - strand at 526.510 kb on + strand at 526.511 kb on - strand at 526.511 kb on - strand at 526.511 kb on - strand at 526.511 kb on - strand at 526.569 kb on - strand at 526.890 kb on + strand, within HER17_RS02340 at 526.890 kb on + strand, within HER17_RS02340 at 526.908 kb on - strand, within HER17_RS02340 at 526.908 kb on - strand, within HER17_RS02340 at 526.908 kb on - strand, within HER17_RS02340 at 526.980 kb on + strand, within HER17_RS02340 at 526.982 kb on + strand, within HER17_RS02340 at 526.982 kb on + strand, within HER17_RS02340 at 526.997 kb on + strand, within HER17_RS02340 at 527.331 kb on - strand, within HER17_RS02340 at 527.436 kb on + strand, within HER17_RS02340 at 527.487 kb on + strand, within HER17_RS02340 at 527.487 kb on + strand, within HER17_RS02340 at 527.488 kb on - strand, within HER17_RS02340 at 527.488 kb on - strand, within HER17_RS02340 at 527.526 kb on - strand, within HER17_RS02340 at 527.526 kb on - strand, within HER17_RS02340 at 527.526 kb on - strand, within HER17_RS02340 at 527.574 kb on + strand, within HER17_RS02340 at 527.575 kb on - strand, within HER17_RS02340 at 527.631 kb on + strand, within HER17_RS02340 at 527.632 kb on - strand, within HER17_RS02340 at 527.768 kb on + strand, within HER17_RS02340 at 527.768 kb on + strand, within HER17_RS02340 at 527.769 kb on - strand, within HER17_RS02340 at 527.852 kb on - strand, within HER17_RS02340 at 527.879 kb on + strand, within HER17_RS02340 at 527.880 kb on - strand, within HER17_RS02340 at 527.931 kb on + strand, within HER17_RS02340 at 527.931 kb on + strand, within HER17_RS02340 at 527.932 kb on - strand, within HER17_RS02340 at 528.002 kb on - strand at 528.050 kb on + strand at 528.051 kb on - strand at 528.074 kb on + strand at 528.171 kb on + strand at 528.172 kb on - strand at 528.172 kb on - strand at 528.318 kb on + strand, within HER17_RS02345 at 528.319 kb on - strand, within HER17_RS02345 at 528.354 kb on + strand, within HER17_RS02345 at 528.355 kb on - strand, within HER17_RS02345 at 528.381 kb on + strand, within HER17_RS02345 at 528.384 kb on + strand, within HER17_RS02345 at 528.385 kb on - strand, within HER17_RS02345 at 528.408 kb on + strand, within HER17_RS02345 at 528.409 kb on - strand, within HER17_RS02345 at 528.415 kb on + strand, within HER17_RS02345 at 528.415 kb on + strand, within HER17_RS02345 at 528.415 kb on + strand, within HER17_RS02345 at 528.416 kb on - strand, within HER17_RS02345 at 528.416 kb on - strand, within HER17_RS02345 at 528.491 kb on - strand at 528.502 kb on + strand at 528.507 kb on + strand at 528.559 kb on + strand at 528.560 kb on - strand at 528.560 kb on - strand at 528.560 kb on - strand at 528.574 kb on + strand at 528.608 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 525,564 + HER17_RS02335 0.71 +0.1 525,716 + HER17_RS02335 0.82 +0.3 525,941 - -0.8 525,996 + -0.3 526,011 + +0.3 526,011 + +0.6 526,067 + +0.5 526,067 + -0.1 526,072 + +1.9 526,072 + -0.8 526,073 - -0.4 526,073 - -0.2 526,073 - +0.6 526,114 + -0.2 526,169 + +0.3 526,237 + +0.7 526,237 + +0.0 526,237 + -0.1 526,237 + -0.0 526,237 + +0.8 526,238 - +0.3 526,238 - +0.1 526,238 - -0.6 526,283 + +0.1 526,291 + +0.1 526,297 + +0.3 526,306 - -1.1 526,306 - +0.6 526,381 - +0.0 526,417 + +0.5 526,417 + +0.1 526,418 - +0.7 526,510 + +0.0 526,511 - +0.6 526,511 - -1.6 526,511 - -0.7 526,511 - +0.2 526,569 - -1.3 526,890 + HER17_RS02340 0.26 -0.8 526,890 + HER17_RS02340 0.26 +0.2 526,908 - HER17_RS02340 0.27 -0.3 526,908 - HER17_RS02340 0.27 -0.2 526,908 - HER17_RS02340 0.27 +0.2 526,980 + HER17_RS02340 0.31 +1.4 526,982 + HER17_RS02340 0.31 -0.9 526,982 + HER17_RS02340 0.31 +0.8 526,997 + HER17_RS02340 0.32 +0.4 527,331 - HER17_RS02340 0.52 -1.8 527,436 + HER17_RS02340 0.59 +0.4 527,487 + HER17_RS02340 0.62 -0.4 527,487 + HER17_RS02340 0.62 -0.6 527,488 - HER17_RS02340 0.62 +0.2 527,488 - HER17_RS02340 0.62 -0.5 527,526 - HER17_RS02340 0.64 -0.7 527,526 - HER17_RS02340 0.64 -0.4 527,526 - HER17_RS02340 0.64 -2.1 527,574 + HER17_RS02340 0.67 -2.7 527,575 - HER17_RS02340 0.67 +1.0 527,631 + HER17_RS02340 0.71 +0.2 527,632 - HER17_RS02340 0.71 -0.6 527,768 + HER17_RS02340 0.79 +0.1 527,768 + HER17_RS02340 0.79 -0.9 527,769 - HER17_RS02340 0.79 +0.7 527,852 - HER17_RS02340 0.84 +0.3 527,879 + HER17_RS02340 0.86 -0.6 527,880 - HER17_RS02340 0.86 +0.1 527,931 + HER17_RS02340 0.89 -0.3 527,931 + HER17_RS02340 0.89 +0.4 527,932 - HER17_RS02340 0.89 -1.1 528,002 - +0.5 528,050 + -0.1 528,051 - -1.0 528,074 + -0.0 528,171 + +0.5 528,172 - +0.2 528,172 - -0.9 528,318 + HER17_RS02345 0.46 -0.4 528,319 - HER17_RS02345 0.46 -0.0 528,354 + HER17_RS02345 0.56 +0.0 528,355 - HER17_RS02345 0.57 +0.4 528,381 + HER17_RS02345 0.64 -0.0 528,384 + HER17_RS02345 0.65 +0.3 528,385 - HER17_RS02345 0.65 -0.2 528,408 + HER17_RS02345 0.72 -0.0 528,409 - HER17_RS02345 0.72 +0.2 528,415 + HER17_RS02345 0.74 +0.2 528,415 + HER17_RS02345 0.74 +0.3 528,415 + HER17_RS02345 0.74 +0.2 528,416 - HER17_RS02345 0.74 +0.9 528,416 - HER17_RS02345 0.74 -0.2 528,491 - +0.7 528,502 + +0.4 528,507 + +0.4 528,559 + +0.8 528,560 - -0.4 528,560 - -1.3 528,560 - -0.2 528,574 + -0.8 528,608 - -0.1
Or see this region's nucleotide sequence