Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS09515 and HER17_RS09520 are separated by 225 nucleotides HER17_RS09520 and HER17_RS09525 are separated by 90 nucleotides HER17_RS09525 and HER17_RS09530 overlap by 1 nucleotides HER17_RS09530 and HER17_RS09535 are separated by 92 nucleotides
HER17_RS09515: HER17_RS09515 - phage shock protein operon transcriptional activator, at 2,092,801 to 2,093,790
_RS09515
HER17_RS09520: HER17_RS09520 - phage shock protein PspA, at 2,094,016 to 2,094,681
_RS09520
HER17_RS09525: HER17_RS09525 - envelope stress response membrane protein PspB, at 2,094,772 to 2,094,996
_RS09525
HER17_RS09530: HER17_RS09530 - envelope stress response membrane protein PspC, at 2,094,996 to 2,095,361
_RS09530
HER17_RS09535: HER17_RS09535 - hypothetical protein, at 2,095,454 to 2,096,008
_RS09535
Position (kb)
2094
2095 Strain fitness (log2 ratio)
-2
-1
0
1 at 2093.115 kb on + strand, within HER17_RS09515 at 2093.115 kb on + strand, within HER17_RS09515 at 2093.163 kb on + strand, within HER17_RS09515 at 2093.218 kb on - strand, within HER17_RS09515 at 2093.314 kb on - strand, within HER17_RS09515 at 2093.377 kb on + strand, within HER17_RS09515 at 2093.378 kb on - strand, within HER17_RS09515 at 2093.560 kb on + strand, within HER17_RS09515 at 2093.639 kb on + strand, within HER17_RS09515 at 2093.827 kb on + strand at 2093.828 kb on - strand at 2093.916 kb on - strand at 2093.919 kb on + strand at 2093.927 kb on + strand at 2093.931 kb on + strand at 2093.984 kb on + strand at 2094.011 kb on + strand at 2094.011 kb on + strand at 2094.011 kb on + strand at 2094.015 kb on + strand at 2094.129 kb on + strand, within HER17_RS09520 at 2094.129 kb on + strand, within HER17_RS09520 at 2094.129 kb on + strand, within HER17_RS09520 at 2094.129 kb on + strand, within HER17_RS09520 at 2094.261 kb on + strand, within HER17_RS09520 at 2094.470 kb on + strand, within HER17_RS09520 at 2094.654 kb on + strand at 2094.654 kb on + strand at 2094.655 kb on - strand at 2094.655 kb on - strand at 2094.666 kb on + strand at 2094.690 kb on - strand at 2094.753 kb on + strand at 2094.753 kb on + strand at 2094.754 kb on - strand at 2094.760 kb on + strand at 2094.761 kb on - strand at 2094.761 kb on - strand at 2094.830 kb on + strand, within HER17_RS09525 at 2094.842 kb on + strand, within HER17_RS09525 at 2094.847 kb on + strand, within HER17_RS09525 at 2094.848 kb on - strand, within HER17_RS09525 at 2094.858 kb on + strand, within HER17_RS09525 at 2094.891 kb on - strand, within HER17_RS09525 at 2094.891 kb on - strand, within HER17_RS09525 at 2094.891 kb on - strand, within HER17_RS09525 at 2094.891 kb on - strand, within HER17_RS09525 at 2094.928 kb on - strand, within HER17_RS09525 at 2095.004 kb on + strand at 2095.005 kb on - strand at 2095.027 kb on - strand at 2095.047 kb on - strand, within HER17_RS09530 at 2095.116 kb on - strand, within HER17_RS09530 at 2095.164 kb on + strand, within HER17_RS09530 at 2095.165 kb on - strand, within HER17_RS09530 at 2095.165 kb on - strand, within HER17_RS09530 at 2095.165 kb on - strand, within HER17_RS09530 at 2095.181 kb on + strand, within HER17_RS09530 at 2095.182 kb on - strand, within HER17_RS09530 at 2095.235 kb on + strand, within HER17_RS09530 at 2095.252 kb on + strand, within HER17_RS09530 at 2095.252 kb on + strand, within HER17_RS09530 at 2095.311 kb on + strand, within HER17_RS09530 at 2095.312 kb on - strand, within HER17_RS09530 at 2095.312 kb on - strand, within HER17_RS09530 at 2095.326 kb on - strand at 2095.360 kb on - strand at 2095.360 kb on - strand at 2095.377 kb on - strand at 2095.455 kb on + strand at 2095.529 kb on + strand, within HER17_RS09535 at 2095.530 kb on - strand, within HER17_RS09535 at 2095.657 kb on + strand, within HER17_RS09535 at 2095.658 kb on - strand, within HER17_RS09535 at 2095.658 kb on - strand, within HER17_RS09535 at 2095.658 kb on - strand, within HER17_RS09535 at 2095.658 kb on - strand, within HER17_RS09535 at 2095.660 kb on + strand, within HER17_RS09535 at 2095.671 kb on + strand, within HER17_RS09535 at 2095.671 kb on + strand, within HER17_RS09535 at 2095.672 kb on - strand, within HER17_RS09535 at 2095.672 kb on - strand, within HER17_RS09535 at 2095.672 kb on - strand, within HER17_RS09535
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,093,115 + HER17_RS09515 0.32 +0.1 2,093,115 + HER17_RS09515 0.32 -0.3 2,093,163 + HER17_RS09515 0.37 -0.6 2,093,218 - HER17_RS09515 0.42 -0.0 2,093,314 - HER17_RS09515 0.52 -0.2 2,093,377 + HER17_RS09515 0.58 +0.4 2,093,378 - HER17_RS09515 0.58 +0.0 2,093,560 + HER17_RS09515 0.77 -0.3 2,093,639 + HER17_RS09515 0.85 +0.0 2,093,827 + -0.0 2,093,828 - +0.5 2,093,916 - -2.0 2,093,919 + -1.2 2,093,927 + +0.5 2,093,931 + -0.4 2,093,984 + +0.1 2,094,011 + +0.5 2,094,011 + +0.4 2,094,011 + -0.0 2,094,015 + -0.0 2,094,129 + HER17_RS09520 0.17 -0.4 2,094,129 + HER17_RS09520 0.17 -2.5 2,094,129 + HER17_RS09520 0.17 +0.2 2,094,129 + HER17_RS09520 0.17 +0.7 2,094,261 + HER17_RS09520 0.37 +0.1 2,094,470 + HER17_RS09520 0.68 +0.4 2,094,654 + +0.0 2,094,654 + +0.3 2,094,655 - -0.7 2,094,655 - +0.6 2,094,666 + +0.4 2,094,690 - -0.0 2,094,753 + -1.5 2,094,753 + -0.0 2,094,754 - +0.1 2,094,760 + +0.0 2,094,761 - -0.0 2,094,761 - -1.1 2,094,830 + HER17_RS09525 0.26 -0.5 2,094,842 + HER17_RS09525 0.31 -0.0 2,094,847 + HER17_RS09525 0.33 -2.0 2,094,848 - HER17_RS09525 0.34 +0.1 2,094,858 + HER17_RS09525 0.38 -0.6 2,094,891 - HER17_RS09525 0.53 -1.0 2,094,891 - HER17_RS09525 0.53 +0.0 2,094,891 - HER17_RS09525 0.53 -0.2 2,094,891 - HER17_RS09525 0.53 +0.3 2,094,928 - HER17_RS09525 0.69 +0.5 2,095,004 + -0.6 2,095,005 - -0.1 2,095,027 - +0.5 2,095,047 - HER17_RS09530 0.14 +0.7 2,095,116 - HER17_RS09530 0.33 -0.0 2,095,164 + HER17_RS09530 0.46 -0.7 2,095,165 - HER17_RS09530 0.46 -0.3 2,095,165 - HER17_RS09530 0.46 +0.3 2,095,165 - HER17_RS09530 0.46 -1.2 2,095,181 + HER17_RS09530 0.51 +0.3 2,095,182 - HER17_RS09530 0.51 -0.8 2,095,235 + HER17_RS09530 0.65 -0.0 2,095,252 + HER17_RS09530 0.70 -0.6 2,095,252 + HER17_RS09530 0.70 +0.9 2,095,311 + HER17_RS09530 0.86 -0.6 2,095,312 - HER17_RS09530 0.86 -0.5 2,095,312 - HER17_RS09530 0.86 -1.1 2,095,326 - -0.4 2,095,360 - -0.3 2,095,360 - +0.2 2,095,377 - +0.8 2,095,455 + +0.1 2,095,529 + HER17_RS09535 0.14 +0.4 2,095,530 - HER17_RS09535 0.14 -0.7 2,095,657 + HER17_RS09535 0.37 +0.0 2,095,658 - HER17_RS09535 0.37 +0.5 2,095,658 - HER17_RS09535 0.37 +0.1 2,095,658 - HER17_RS09535 0.37 -0.1 2,095,658 - HER17_RS09535 0.37 -0.3 2,095,660 + HER17_RS09535 0.37 -0.7 2,095,671 + HER17_RS09535 0.39 -1.5 2,095,671 + HER17_RS09535 0.39 +0.3 2,095,672 - HER17_RS09535 0.39 -0.7 2,095,672 - HER17_RS09535 0.39 +0.0 2,095,672 - HER17_RS09535 0.39 -0.3
Or see this region's nucleotide sequence