Strain Fitness in Escherichia coli BL21 around ECD_01253

Experiment: Tailocin 264

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01253 and ECD_01254 are separated by 63 nucleotidesECD_01254 and ECD_01255 are separated by 169 nucleotides ECD_01253: ECD_01253 - aconitate hydratase 1; aconitase A, at 1,322,201 to 1,324,876 _01253 ECD_01254: ECD_01254 - GTP cyclohydrolase II, at 1,324,940 to 1,325,530 _01254 ECD_01255: ECD_01255 - phosphatidylglycerophosphatase B, at 1,325,700 to 1,326,464 _01255 Position (kb) 1322 1323 1324 1325Strain fitness (log2 ratio) -2 -1 0 1at 1321.464 kb on + strandat 1321.470 kb on + strandat 1321.660 kb on + strandat 1321.660 kb on + strandat 1321.660 kb on + strandat 1321.660 kb on + strandat 1321.705 kb on + strandat 1321.791 kb on + strandat 1321.941 kb on + strandat 1322.201 kb on - strandat 1322.267 kb on + strandat 1322.669 kb on + strand, within ECD_01253at 1322.946 kb on + strand, within ECD_01253at 1323.465 kb on + strand, within ECD_01253at 1324.091 kb on + strand, within ECD_01253at 1324.425 kb on + strand, within ECD_01253at 1324.440 kb on + strand, within ECD_01253at 1324.451 kb on + strand, within ECD_01253at 1325.545 kb on - strandat 1325.545 kb on - strandat 1325.545 kb on - strandat 1325.567 kb on - strandat 1325.667 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Tailocin 264
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1,321,464 + +0.8
1,321,470 + -0.1
1,321,660 + -2.4
1,321,660 + +0.4
1,321,660 + +0.2
1,321,660 + +0.7
1,321,705 + +0.2
1,321,791 + -0.5
1,321,941 + +0.3
1,322,201 - +0.4
1,322,267 + +0.6
1,322,669 + ECD_01253 0.17 -1.2
1,322,946 + ECD_01253 0.28 -0.6
1,323,465 + ECD_01253 0.47 -1.1
1,324,091 + ECD_01253 0.71 +0.6
1,324,425 + ECD_01253 0.83 -1.8
1,324,440 + ECD_01253 0.84 -0.7
1,324,451 + ECD_01253 0.84 -0.9
1,325,545 - -0.5
1,325,545 - -0.2
1,325,545 - -1.4
1,325,567 - +0.3
1,325,667 + +0.9

Or see this region's nucleotide sequence