Strain Fitness in Escherichia coli BL21 around ECD_01160

Experiment: Tailocin 255

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01159 and ECD_01160 are separated by 45 nucleotidesECD_01160 and ECD_01161 are separated by 145 nucleotides ECD_01159: ECD_01159 - translesion error-prone DNA polymerase V subunit; DNA polymerase activity, at 1,219,334 to 1,220,602 _01159 ECD_01160: ECD_01160 - oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I, at 1,220,648 to 1,221,178 _01160 ECD_01161: ECD_01161 - sodium:proton antiporter, at 1,221,324 to 1,222,865 _01161 Position (kb) 1220 1221 1222Strain fitness (log2 ratio) -3 -2 -1 0 1at 1219.857 kb on + strand, within ECD_01159at 1219.857 kb on + strand, within ECD_01159at 1220.567 kb on - strandat 1220.918 kb on + strand, within ECD_01160at 1220.919 kb on - strand, within ECD_01160at 1220.942 kb on - strand, within ECD_01160at 1220.943 kb on + strand, within ECD_01160at 1220.943 kb on + strand, within ECD_01160at 1220.944 kb on - strand, within ECD_01160at 1220.944 kb on - strand, within ECD_01160at 1220.944 kb on - strand, within ECD_01160at 1220.967 kb on + strand, within ECD_01160at 1221.042 kb on + strand, within ECD_01160at 1221.047 kb on - strand, within ECD_01160at 1221.304 kb on - strandat 1221.425 kb on - strandat 1221.632 kb on - strand, within ECD_01161at 1222.102 kb on - strand, within ECD_01161

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Per-strain Table

Position Strand Gene LocusTag Fraction Tailocin 255
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1,219,857 + ECD_01159 0.41 +0.4
1,219,857 + ECD_01159 0.41 +0.8
1,220,567 - -1.8
1,220,918 + ECD_01160 0.51 -0.8
1,220,919 - ECD_01160 0.51 -0.3
1,220,942 - ECD_01160 0.55 +0.2
1,220,943 + ECD_01160 0.56 -1.6
1,220,943 + ECD_01160 0.56 -2.4
1,220,944 - ECD_01160 0.56 -2.2
1,220,944 - ECD_01160 0.56 -2.9
1,220,944 - ECD_01160 0.56 -1.2
1,220,967 + ECD_01160 0.60 -1.9
1,221,042 + ECD_01160 0.74 -1.8
1,221,047 - ECD_01160 0.75 -2.2
1,221,304 - +0.5
1,221,425 - -0.2
1,221,632 - ECD_01161 0.20 -0.1
1,222,102 - ECD_01161 0.50 +0.1

Or see this region's nucleotide sequence