Experiment: 4-Hydroxybenzoic Acid 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5026 and Atu5027 are separated by 120 nucleotides Atu5027 and Atu5028 overlap by 4 nucleotides Atu5028 and Atu5029 are separated by 170 nucleotides
Atu5026: Atu5026 - hypothetical protein, at 28,294 to 28,704
Atu5026
Atu5027: Atu5027 - IS 426 transposase, at 28,825 to 29,379
Atu5027
Atu5028: Atu5028 - IS426 transposase, at 29,376 to 29,753
Atu5028
Atu5029: Atu5029 - hypothetical protein, at 29,924 to 30,694
Atu5029
Position (kb)
29
30 Strain fitness (log2 ratio)
-2
-1
0
1 at 28.386 kb on - strand, within Atu5026 at 28.399 kb on + strand, within Atu5026 at 28.407 kb on - strand, within Atu5026 at 28.407 kb on - strand, within Atu5026 at 28.517 kb on - strand, within Atu5026 at 28.639 kb on + strand, within Atu5026 at 28.641 kb on - strand, within Atu5026 at 28.684 kb on - strand at 28.684 kb on - strand at 28.809 kb on + strand at 28.846 kb on - strand at 28.848 kb on + strand at 28.848 kb on + strand at 28.856 kb on + strand at 28.856 kb on - strand at 28.856 kb on - strand at 28.856 kb on - strand at 28.856 kb on - strand at 28.864 kb on - strand at 28.911 kb on + strand, within Atu5027 at 28.911 kb on + strand, within Atu5027 at 28.911 kb on + strand, within Atu5027 at 28.919 kb on - strand, within Atu5027 at 28.919 kb on - strand, within Atu5027 at 28.919 kb on - strand, within Atu5027 at 28.999 kb on - strand, within Atu5027 at 29.022 kb on + strand, within Atu5027 at 29.023 kb on + strand, within Atu5027 at 29.030 kb on - strand, within Atu5027 at 29.044 kb on + strand, within Atu5027 at 29.044 kb on + strand, within Atu5027 at 29.052 kb on - strand, within Atu5027 at 29.052 kb on - strand, within Atu5027 at 29.056 kb on + strand, within Atu5027 at 29.063 kb on + strand, within Atu5027 at 29.063 kb on + strand, within Atu5027 at 29.071 kb on - strand, within Atu5027 at 29.071 kb on - strand, within Atu5027 at 29.078 kb on - strand, within Atu5027 at 29.105 kb on + strand, within Atu5027 at 29.108 kb on - strand, within Atu5027 at 29.110 kb on - strand, within Atu5027 at 29.124 kb on - strand, within Atu5027 at 29.166 kb on + strand, within Atu5027 at 29.167 kb on - strand, within Atu5027 at 29.172 kb on - strand, within Atu5027 at 29.180 kb on - strand, within Atu5027 at 29.180 kb on - strand, within Atu5027 at 29.190 kb on - strand, within Atu5027 at 29.203 kb on - strand, within Atu5027 at 29.203 kb on - strand, within Atu5027 at 29.205 kb on - strand, within Atu5027 at 29.360 kb on - strand at 29.441 kb on - strand, within Atu5028 at 29.629 kb on + strand, within Atu5028 at 29.697 kb on - strand, within Atu5028 at 29.697 kb on - strand, within Atu5028 at 29.697 kb on - strand, within Atu5028 at 29.697 kb on - strand, within Atu5028 at 29.750 kb on + strand at 29.756 kb on + strand at 29.762 kb on + strand at 29.762 kb on + strand at 29.762 kb on + strand at 29.762 kb on + strand at 29.764 kb on - strand at 29.770 kb on - strand at 29.770 kb on - strand at 29.780 kb on - strand at 29.846 kb on - strand at 29.923 kb on - strand at 29.938 kb on + strand at 29.983 kb on - strand at 30.003 kb on - strand, within Atu5029 at 30.074 kb on + strand, within Atu5029 at 30.089 kb on - strand, within Atu5029 at 30.089 kb on - strand, within Atu5029 at 30.094 kb on - strand, within Atu5029 at 30.128 kb on + strand, within Atu5029 at 30.183 kb on - strand, within Atu5029 at 30.206 kb on - strand, within Atu5029 at 30.208 kb on + strand, within Atu5029 at 30.268 kb on + strand, within Atu5029 at 30.276 kb on - strand, within Atu5029 at 30.276 kb on - strand, within Atu5029 at 30.294 kb on + strand, within Atu5029 at 30.321 kb on - strand, within Atu5029 at 30.338 kb on - strand, within Atu5029 at 30.347 kb on - strand, within Atu5029 at 30.416 kb on + strand, within Atu5029 at 30.562 kb on - strand, within Atu5029 at 30.581 kb on + strand, within Atu5029 at 30.644 kb on - strand at 30.706 kb on - strand at 30.706 kb on - strand at 30.725 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid 5 mM remove 28,386 - Atu5026 0.22 -0.8 28,399 + Atu5026 0.26 -0.2 28,407 - Atu5026 0.27 -0.5 28,407 - Atu5026 0.27 -0.1 28,517 - Atu5026 0.54 +0.2 28,639 + Atu5026 0.84 -0.2 28,641 - Atu5026 0.84 -0.0 28,684 - +0.5 28,684 - +0.0 28,809 + -0.4 28,846 - +0.2 28,848 + +1.3 28,848 + +0.6 28,856 + -0.1 28,856 - -0.2 28,856 - -0.3 28,856 - -0.1 28,856 - -0.4 28,864 - -0.2 28,911 + Atu5027 0.15 -1.1 28,911 + Atu5027 0.15 -0.1 28,911 + Atu5027 0.15 -1.1 28,919 - Atu5027 0.17 +1.3 28,919 - Atu5027 0.17 +0.0 28,919 - Atu5027 0.17 +0.6 28,999 - Atu5027 0.31 -0.0 29,022 + Atu5027 0.35 -0.6 29,023 + Atu5027 0.36 +0.1 29,030 - Atu5027 0.37 -0.2 29,044 + Atu5027 0.39 -0.1 29,044 + Atu5027 0.39 -1.1 29,052 - Atu5027 0.41 -0.5 29,052 - Atu5027 0.41 +1.2 29,056 + Atu5027 0.42 +0.3 29,063 + Atu5027 0.43 -1.5 29,063 + Atu5027 0.43 +0.5 29,071 - Atu5027 0.44 +0.1 29,071 - Atu5027 0.44 +0.2 29,078 - Atu5027 0.46 -0.2 29,105 + Atu5027 0.50 +0.4 29,108 - Atu5027 0.51 -0.0 29,110 - Atu5027 0.51 +1.6 29,124 - Atu5027 0.54 -0.2 29,166 + Atu5027 0.61 -0.0 29,167 - Atu5027 0.62 +0.1 29,172 - Atu5027 0.63 -0.7 29,180 - Atu5027 0.64 +0.6 29,180 - Atu5027 0.64 +0.1 29,190 - Atu5027 0.66 -0.3 29,203 - Atu5027 0.68 -0.1 29,203 - Atu5027 0.68 +0.1 29,205 - Atu5027 0.68 -0.4 29,360 - +0.1 29,441 - Atu5028 0.17 +0.2 29,629 + Atu5028 0.67 +1.6 29,697 - Atu5028 0.85 +0.1 29,697 - Atu5028 0.85 +0.0 29,697 - Atu5028 0.85 -0.6 29,697 - Atu5028 0.85 +1.0 29,750 + -0.3 29,756 + -0.3 29,762 + +0.3 29,762 + +0.1 29,762 + -1.8 29,762 + +1.3 29,764 - -0.1 29,770 - +0.0 29,770 - +0.2 29,780 - -0.4 29,846 - -0.3 29,923 - +0.5 29,938 + +0.1 29,983 - -0.2 30,003 - Atu5029 0.10 -0.1 30,074 + Atu5029 0.19 -0.3 30,089 - Atu5029 0.21 -0.3 30,089 - Atu5029 0.21 -0.5 30,094 - Atu5029 0.22 +0.1 30,128 + Atu5029 0.26 -0.1 30,183 - Atu5029 0.34 -0.2 30,206 - Atu5029 0.37 -0.4 30,208 + Atu5029 0.37 -0.2 30,268 + Atu5029 0.45 +0.8 30,276 - Atu5029 0.46 -0.7 30,276 - Atu5029 0.46 -0.2 30,294 + Atu5029 0.48 -0.4 30,321 - Atu5029 0.51 -0.4 30,338 - Atu5029 0.54 +0.4 30,347 - Atu5029 0.55 -1.0 30,416 + Atu5029 0.64 -0.0 30,562 - Atu5029 0.83 -0.2 30,581 + Atu5029 0.85 -0.2 30,644 - +0.7 30,706 - -0.1 30,706 - -0.2 30,725 + +1.0
Or see this region's nucleotide sequence