Experiment: 4-Hydroxybenzoic Acid 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3472 and Atu3473 are separated by 3 nucleotides Atu3473 and Atu3474 are separated by 44 nucleotides
Atu3472: Atu3472 - 2-oxoisovalerate dehydrogenase subunit beta, at 519,185 to 520,198
Atu3472
Atu3473: Atu3473 - 2-oxoisovalerate dehydrogenase subunit alpha, at 520,202 to 521,440
Atu3473
Atu3474: Atu3474 - acyl-CoA dehydrogenase, at 521,485 to 522,612
Atu3474
Position (kb) 520
521
522 Strain fitness (log2 ratio) -1
0
1 at 519.419 kb on - strand, within Atu3472 at 519.485 kb on - strand, within Atu3472 at 519.699 kb on + strand, within Atu3472 at 519.700 kb on + strand, within Atu3472 at 519.774 kb on - strand, within Atu3472 at 519.930 kb on - strand, within Atu3472 at 519.984 kb on - strand, within Atu3472 at 520.078 kb on - strand, within Atu3472 at 520.149 kb on + strand at 520.381 kb on + strand, within Atu3473 at 520.402 kb on + strand, within Atu3473 at 520.403 kb on - strand, within Atu3473 at 520.461 kb on + strand, within Atu3473 at 520.501 kb on - strand, within Atu3473 at 520.534 kb on + strand, within Atu3473 at 520.542 kb on - strand, within Atu3473 at 520.547 kb on - strand, within Atu3473 at 520.572 kb on - strand, within Atu3473 at 520.583 kb on + strand, within Atu3473 at 520.583 kb on + strand, within Atu3473 at 520.591 kb on - strand, within Atu3473 at 520.591 kb on - strand, within Atu3473 at 520.629 kb on - strand, within Atu3473 at 520.739 kb on - strand, within Atu3473 at 520.931 kb on - strand, within Atu3473 at 520.979 kb on - strand, within Atu3473 at 521.134 kb on - strand, within Atu3473 at 521.226 kb on + strand, within Atu3473 at 521.282 kb on + strand, within Atu3473 at 521.290 kb on - strand, within Atu3473 at 521.300 kb on + strand, within Atu3473 at 521.334 kb on + strand at 521.342 kb on - strand at 521.459 kb on + strand at 521.463 kb on + strand at 521.464 kb on + strand at 521.467 kb on - strand at 521.509 kb on + strand at 521.528 kb on - strand at 521.566 kb on - strand at 521.694 kb on - strand, within Atu3474 at 521.699 kb on - strand, within Atu3474 at 521.746 kb on - strand, within Atu3474 at 521.803 kb on + strand, within Atu3474 at 521.838 kb on - strand, within Atu3474 at 521.848 kb on - strand, within Atu3474 at 521.929 kb on - strand, within Atu3474 at 521.946 kb on + strand, within Atu3474 at 521.954 kb on - strand, within Atu3474 at 522.029 kb on + strand, within Atu3474 at 522.034 kb on - strand, within Atu3474 at 522.146 kb on - strand, within Atu3474 at 522.156 kb on + strand, within Atu3474 at 522.164 kb on - strand, within Atu3474 at 522.174 kb on - strand, within Atu3474 at 522.174 kb on - strand, within Atu3474 at 522.193 kb on - strand, within Atu3474 at 522.203 kb on + strand, within Atu3474 at 522.210 kb on - strand, within Atu3474 at 522.244 kb on - strand, within Atu3474
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid 5 mM remove 519,419 - Atu3472 0.23 +0.7 519,485 - Atu3472 0.30 +0.0 519,699 + Atu3472 0.51 -0.3 519,700 + Atu3472 0.51 +1.3 519,774 - Atu3472 0.58 +0.4 519,930 - Atu3472 0.73 -1.0 519,984 - Atu3472 0.79 +0.2 520,078 - Atu3472 0.88 +0.4 520,149 + -0.1 520,381 + Atu3473 0.14 -0.1 520,402 + Atu3473 0.16 +0.4 520,403 - Atu3473 0.16 +0.6 520,461 + Atu3473 0.21 +0.2 520,501 - Atu3473 0.24 -0.3 520,534 + Atu3473 0.27 -1.3 520,542 - Atu3473 0.27 -0.2 520,547 - Atu3473 0.28 +0.7 520,572 - Atu3473 0.30 -0.4 520,583 + Atu3473 0.31 +0.3 520,583 + Atu3473 0.31 +0.8 520,591 - Atu3473 0.31 -0.2 520,591 - Atu3473 0.31 +0.2 520,629 - Atu3473 0.34 +0.1 520,739 - Atu3473 0.43 -0.0 520,931 - Atu3473 0.59 -0.2 520,979 - Atu3473 0.63 +0.5 521,134 - Atu3473 0.75 -0.1 521,226 + Atu3473 0.83 -0.3 521,282 + Atu3473 0.87 -0.4 521,290 - Atu3473 0.88 +0.5 521,300 + Atu3473 0.89 -0.1 521,334 + +0.1 521,342 - -0.1 521,459 + -0.2 521,463 + -0.3 521,464 + +0.8 521,467 - +0.4 521,509 + -1.1 521,528 - -0.3 521,566 - +0.5 521,694 - Atu3474 0.19 +0.6 521,699 - Atu3474 0.19 -1.1 521,746 - Atu3474 0.23 +0.1 521,803 + Atu3474 0.28 +0.4 521,838 - Atu3474 0.31 +0.4 521,848 - Atu3474 0.32 +1.0 521,929 - Atu3474 0.39 -0.0 521,946 + Atu3474 0.41 -0.1 521,954 - Atu3474 0.42 -0.1 522,029 + Atu3474 0.48 +0.5 522,034 - Atu3474 0.49 -0.1 522,146 - Atu3474 0.59 +0.1 522,156 + Atu3474 0.59 -0.1 522,164 - Atu3474 0.60 -0.1 522,174 - Atu3474 0.61 +0.1 522,174 - Atu3474 0.61 +0.0 522,193 - Atu3474 0.63 -0.3 522,203 + Atu3474 0.64 -0.5 522,210 - Atu3474 0.64 -1.4 522,244 - Atu3474 0.67 -0.2
Or see this region's nucleotide sequence