Experiment: pH 9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1049 and PP_1050 are separated by 3 nucleotides PP_1050 and PP_1051 overlap by 43 nucleotides PP_1051 and PP_1052 overlap by 4 nucleotides PP_1052 and PP_1053 overlap by 16 nucleotides
PP_1049: PP_1049 - putative protein secretion protein for export, at 1,200,345 to 1,200,800
_1049
PP_1050: PP_1050 - Type II secretion pathway protein XcpU, at 1,200,804 to 1,201,235
_1050
PP_1051: PP_1051 - Type II secretion pathway protein XcpV, at 1,201,193 to 1,201,600
_1051
PP_1052: PP_1052 - Type II secretion pathway protein XcpW, at 1,201,597 to 1,202,187
_1052
PP_1053: PP_1053 - Type II secretion pathway protein XcpY, at 1,202,172 to 1,203,260
_1053
Position (kb)
1201
1202
1203 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1200.648 kb on + strand, within PP_1049 at 1200.649 kb on - strand, within PP_1049 at 1200.688 kb on - strand, within PP_1049 at 1200.688 kb on - strand, within PP_1049 at 1200.730 kb on - strand, within PP_1049 at 1200.798 kb on + strand at 1200.799 kb on - strand at 1201.157 kb on + strand, within PP_1050 at 1201.275 kb on + strand, within PP_1051 at 1201.320 kb on + strand, within PP_1051 at 1201.321 kb on - strand, within PP_1051 at 1201.418 kb on + strand, within PP_1051 at 1201.419 kb on - strand, within PP_1051 at 1201.574 kb on + strand at 1201.575 kb on - strand at 1201.575 kb on - strand at 1201.575 kb on - strand at 1201.575 kb on - strand at 1201.984 kb on + strand, within PP_1052 at 1202.149 kb on + strand at 1202.149 kb on + strand at 1202.149 kb on + strand at 1202.150 kb on - strand at 1202.150 kb on - strand at 1202.208 kb on - strand at 1202.560 kb on + strand, within PP_1053 at 1202.646 kb on + strand, within PP_1053 at 1202.647 kb on - strand at 1202.771 kb on - strand, within PP_1053 at 1203.023 kb on - strand, within PP_1053 at 1203.023 kb on - strand, within PP_1053 at 1203.041 kb on + strand, within PP_1053 at 1203.041 kb on + strand, within PP_1053 at 1203.042 kb on - strand, within PP_1053 at 1203.042 kb on - strand, within PP_1053 at 1203.053 kb on - strand, within PP_1053 at 1203.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pH 9 remove 1,200,648 + PP_1049 0.66 +1.2 1,200,649 - PP_1049 0.67 -0.6 1,200,688 - PP_1049 0.75 -0.5 1,200,688 - PP_1049 0.75 -2.3 1,200,730 - PP_1049 0.84 +0.0 1,200,798 + +0.0 1,200,799 - +1.7 1,201,157 + PP_1050 0.82 -0.1 1,201,275 + PP_1051 0.20 +1.3 1,201,320 + PP_1051 0.31 -2.7 1,201,321 - PP_1051 0.31 +0.0 1,201,418 + PP_1051 0.55 -1.7 1,201,419 - PP_1051 0.55 -2.1 1,201,574 + +2.8 1,201,575 - -1.5 1,201,575 - -1.2 1,201,575 - +2.6 1,201,575 - +2.0 1,201,984 + PP_1052 0.65 +0.4 1,202,149 + -0.8 1,202,149 + +0.4 1,202,149 + -0.8 1,202,150 - +0.4 1,202,150 - +1.4 1,202,208 - -3.3 1,202,560 + PP_1053 0.36 +1.0 1,202,646 + PP_1053 0.44 +0.4 1,202,647 - +1.4 1,202,771 - PP_1053 0.55 +0.1 1,203,023 - PP_1053 0.78 -1.8 1,203,023 - PP_1053 0.78 +1.0 1,203,041 + PP_1053 0.80 -2.2 1,203,041 + PP_1053 0.80 +0.4 1,203,042 - PP_1053 0.80 -2.3 1,203,042 - PP_1053 0.80 +0.3 1,203,053 - PP_1053 0.81 +1.5 1,203,185 + -1.0
Or see this region's nucleotide sequence