Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0627 and PP_0628 are separated by 132 nucleotides PP_0628 and PP_5447 are separated by 55 nucleotides PP_5447 and PP_0629 are separated by 86 nucleotides PP_0629 and PP_0630 are separated by 111 nucleotides
PP_0627: PP_0627 - conserved protein of unknown function, at 735,401 to 735,574
_0627
PP_0628: PP_0628 - conserved protein of unknown function, at 735,707 to 736,336
_0628
PP_5447: PP_5447 - conserved membrane protein of unknown function, at 736,392 to 736,823
_5447
PP_0629: PP_0629 - conserved membrane protein of unknown function, at 736,910 to 737,617
_0629
PP_0630: PP_0630 - DNA gyrase inhibitor YacG, at 737,729 to 737,929
_0630
Position (kb) 736
737 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 735.405 kb on - strand at 735.405 kb on - strand at 735.405 kb on - strand at 735.528 kb on + strand, within PP_0627 at 735.528 kb on + strand, within PP_0627 at 735.529 kb on - strand, within PP_0627 at 735.529 kb on - strand, within PP_0627 at 735.591 kb on + strand at 735.659 kb on + strand at 735.677 kb on + strand at 735.678 kb on - strand at 735.722 kb on + strand at 735.722 kb on + strand at 735.723 kb on - strand at 735.846 kb on + strand, within PP_0628 at 735.847 kb on - strand, within PP_0628 at 736.204 kb on - strand, within PP_0628 at 736.261 kb on + strand, within PP_0628 at 736.262 kb on - strand, within PP_0628 at 736.306 kb on + strand at 736.307 kb on - strand at 736.307 kb on - strand at 736.320 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.369 kb on - strand at 736.475 kb on + strand, within PP_5447 at 736.475 kb on + strand, within PP_5447 at 736.509 kb on + strand, within PP_5447 at 736.537 kb on + strand, within PP_5447 at 736.537 kb on + strand, within PP_5447 at 736.537 kb on + strand, within PP_5447 at 736.538 kb on - strand, within PP_5447 at 736.538 kb on - strand, within PP_5447 at 736.613 kb on - strand, within PP_5447 at 736.724 kb on - strand, within PP_5447 at 736.761 kb on + strand, within PP_5447 at 736.761 kb on + strand, within PP_5447 at 736.794 kb on + strand at 736.892 kb on - strand at 736.892 kb on - strand at 736.903 kb on + strand at 736.903 kb on + strand at 736.925 kb on - strand at 736.925 kb on - strand at 736.988 kb on + strand, within PP_0629 at 736.989 kb on - strand, within PP_0629 at 737.135 kb on + strand, within PP_0629 at 737.144 kb on - strand, within PP_0629 at 737.297 kb on - strand, within PP_0629 at 737.314 kb on - strand, within PP_0629 at 737.314 kb on - strand, within PP_0629 at 737.354 kb on - strand, within PP_0629 at 737.526 kb on + strand, within PP_0629 at 737.526 kb on + strand, within PP_0629 at 737.526 kb on + strand, within PP_0629 at 737.582 kb on + strand at 737.714 kb on + strand at 737.714 kb on + strand at 737.714 kb on + strand at 737.714 kb on + strand at 737.715 kb on - strand at 737.715 kb on - strand at 737.715 kb on - strand at 737.757 kb on + strand, within PP_0630
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 735,405 - -0.4 735,405 - -2.3 735,405 - -0.3 735,528 + PP_0627 0.73 -2.7 735,528 + PP_0627 0.73 -2.0 735,529 - PP_0627 0.74 +1.4 735,529 - PP_0627 0.74 -0.1 735,591 + -1.7 735,659 + -1.0 735,677 + -1.6 735,678 - +0.8 735,722 + -1.5 735,722 + +1.8 735,723 - -0.8 735,846 + PP_0628 0.22 +0.2 735,847 - PP_0628 0.22 +2.6 736,204 - PP_0628 0.79 -2.0 736,261 + PP_0628 0.88 +3.0 736,262 - PP_0628 0.88 -3.2 736,306 + +0.8 736,307 - +0.6 736,307 - +0.9 736,320 + -0.6 736,368 + +0.8 736,368 + +0.2 736,368 + -1.0 736,368 + +0.5 736,368 + -2.4 736,368 + +0.8 736,368 + -1.1 736,368 + -2.5 736,369 - +2.4 736,475 + PP_5447 0.19 +0.2 736,475 + PP_5447 0.19 -3.3 736,509 + PP_5447 0.27 +0.1 736,537 + PP_5447 0.34 -0.6 736,537 + PP_5447 0.34 -0.8 736,537 + PP_5447 0.34 -1.3 736,538 - PP_5447 0.34 -2.2 736,538 - PP_5447 0.34 -1.3 736,613 - PP_5447 0.51 -1.6 736,724 - PP_5447 0.77 -0.7 736,761 + PP_5447 0.85 -1.3 736,761 + PP_5447 0.85 -0.8 736,794 + -3.0 736,892 - +0.4 736,892 - -0.1 736,903 + -0.9 736,903 + +0.8 736,925 - +0.4 736,925 - +1.6 736,988 + PP_0629 0.11 -1.0 736,989 - PP_0629 0.11 -0.4 737,135 + PP_0629 0.32 +0.8 737,144 - PP_0629 0.33 -0.4 737,297 - PP_0629 0.55 +0.0 737,314 - PP_0629 0.57 -1.2 737,314 - PP_0629 0.57 +0.8 737,354 - PP_0629 0.63 +0.9 737,526 + PP_0629 0.87 +0.3 737,526 + PP_0629 0.87 +0.8 737,526 + PP_0629 0.87 +0.8 737,582 + +0.5 737,714 + -2.7 737,714 + +0.8 737,714 + -1.5 737,714 + +0.8 737,715 - +1.0 737,715 - +1.3 737,715 - +0.1 737,757 + PP_0630 0.14 -0.5
Or see this region's nucleotide sequence