Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5165 and PP_5166 are separated by 175 nucleotides PP_5166 and PP_5167 are separated by 64 nucleotides PP_5167 and PP_5168 are separated by 210 nucleotides
PP_5165: PP_5165 - NLPA lipoprotein, at 5,890,832 to 5,891,617
_5165
PP_5166: PP_5166 - Sigma-54 dependent transcriptional regulator, at 5,891,793 to 5,892,623
_5166
PP_5167: PP_5167 - conserved hypothetical protein, at 5,892,688 to 5,893,269
_5167
PP_5168: PP_5168 - sulfate/thiosulfate import ATP-binding protein, at 5,893,480 to 5,894,469
_5168
Position (kb)
5891
5892
5893 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 5890.811 kb on + strand at 5890.811 kb on + strand at 5890.811 kb on + strand at 5890.812 kb on - strand at 5890.812 kb on - strand at 5890.812 kb on - strand at 5890.812 kb on - strand at 5890.812 kb on - strand at 5890.812 kb on - strand at 5890.848 kb on - strand at 5890.860 kb on + strand at 5890.860 kb on + strand at 5890.968 kb on + strand, within PP_5165 at 5890.968 kb on + strand, within PP_5165 at 5890.969 kb on - strand, within PP_5165 at 5891.034 kb on + strand, within PP_5165 at 5891.034 kb on + strand, within PP_5165 at 5891.035 kb on - strand, within PP_5165 at 5891.176 kb on + strand, within PP_5165 at 5891.177 kb on - strand, within PP_5165 at 5891.177 kb on - strand, within PP_5165 at 5891.309 kb on + strand, within PP_5165 at 5891.309 kb on + strand, within PP_5165 at 5891.309 kb on + strand, within PP_5165 at 5891.309 kb on + strand, within PP_5165 at 5891.310 kb on - strand, within PP_5165 at 5891.310 kb on - strand, within PP_5165 at 5891.310 kb on - strand, within PP_5165 at 5891.315 kb on + strand, within PP_5165 at 5891.316 kb on - strand, within PP_5165 at 5891.316 kb on - strand, within PP_5165 at 5891.370 kb on + strand at 5891.701 kb on + strand at 5891.739 kb on - strand at 5891.739 kb on - strand at 5891.747 kb on - strand at 5891.764 kb on - strand at 5891.767 kb on + strand at 5891.794 kb on + strand at 5891.794 kb on + strand at 5891.794 kb on + strand at 5891.794 kb on + strand at 5891.794 kb on + strand at 5891.794 kb on + strand at 5891.794 kb on + strand at 5891.794 kb on + strand at 5891.795 kb on - strand at 5891.795 kb on - strand at 5891.795 kb on - strand at 5891.974 kb on + strand, within PP_5166 at 5891.974 kb on + strand, within PP_5166 at 5891.974 kb on + strand, within PP_5166 at 5891.975 kb on - strand, within PP_5166 at 5891.975 kb on - strand, within PP_5166 at 5891.989 kb on + strand, within PP_5166 at 5892.068 kb on - strand, within PP_5166 at 5892.250 kb on + strand, within PP_5166 at 5892.251 kb on - strand, within PP_5166 at 5892.324 kb on - strand, within PP_5166 at 5892.349 kb on + strand, within PP_5166 at 5892.350 kb on - strand, within PP_5166 at 5892.531 kb on - strand, within PP_5166 at 5892.591 kb on + strand at 5892.592 kb on - strand at 5892.679 kb on + strand at 5892.679 kb on + strand at 5892.735 kb on - strand at 5892.741 kb on - strand at 5892.840 kb on - strand, within PP_5167 at 5892.840 kb on - strand, within PP_5167 at 5893.006 kb on + strand, within PP_5167 at 5893.007 kb on - strand, within PP_5167 at 5893.248 kb on - strand at 5893.248 kb on - strand at 5893.284 kb on + strand at 5893.284 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.308 kb on + strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.309 kb on - strand at 5893.310 kb on + strand at 5893.310 kb on + strand at 5893.311 kb on - strand at 5893.311 kb on - strand at 5893.407 kb on + strand at 5893.407 kb on + strand at 5893.408 kb on - strand at 5893.408 kb on - strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.485 kb on + strand at 5893.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 5,890,811 + -0.3 5,890,811 + +0.4 5,890,811 + +3.1 5,890,812 - -3.9 5,890,812 - +2.8 5,890,812 - -0.0 5,890,812 - +2.3 5,890,812 - -0.2 5,890,812 - -3.9 5,890,848 - -2.9 5,890,860 + -1.1 5,890,860 + -0.8 5,890,968 + PP_5165 0.17 +1.1 5,890,968 + PP_5165 0.17 +0.4 5,890,969 - PP_5165 0.17 +2.3 5,891,034 + PP_5165 0.26 -0.9 5,891,034 + PP_5165 0.26 -0.2 5,891,035 - PP_5165 0.26 +2.8 5,891,176 + PP_5165 0.44 -0.5 5,891,177 - PP_5165 0.44 +2.3 5,891,177 - PP_5165 0.44 +0.9 5,891,309 + PP_5165 0.61 -0.8 5,891,309 + PP_5165 0.61 -2.0 5,891,309 + PP_5165 0.61 -2.6 5,891,309 + PP_5165 0.61 -0.2 5,891,310 - PP_5165 0.61 +0.1 5,891,310 - PP_5165 0.61 -0.6 5,891,310 - PP_5165 0.61 +2.1 5,891,315 + PP_5165 0.61 -0.9 5,891,316 - PP_5165 0.62 -2.1 5,891,316 - PP_5165 0.62 +1.5 5,891,370 + -0.8 5,891,701 + +1.1 5,891,739 - +0.8 5,891,739 - -0.6 5,891,747 - +0.8 5,891,764 - -1.0 5,891,767 + +0.8 5,891,794 + +0.0 5,891,794 + -0.7 5,891,794 + +3.9 5,891,794 + -4.0 5,891,794 + -0.2 5,891,794 + -0.0 5,891,794 + -0.8 5,891,794 + +0.8 5,891,795 - -1.3 5,891,795 - +1.8 5,891,795 - -0.1 5,891,974 + PP_5166 0.22 +1.3 5,891,974 + PP_5166 0.22 +3.9 5,891,974 + PP_5166 0.22 +2.9 5,891,975 - PP_5166 0.22 +0.9 5,891,975 - PP_5166 0.22 +0.2 5,891,989 + PP_5166 0.24 +1.6 5,892,068 - PP_5166 0.33 -0.0 5,892,250 + PP_5166 0.55 -0.6 5,892,251 - PP_5166 0.55 +2.4 5,892,324 - PP_5166 0.64 -2.1 5,892,349 + PP_5166 0.67 +0.8 5,892,350 - PP_5166 0.67 -1.9 5,892,531 - PP_5166 0.89 +1.4 5,892,591 + +1.0 5,892,592 - +1.0 5,892,679 + +5.1 5,892,679 + +0.7 5,892,735 - -2.1 5,892,741 - +2.1 5,892,840 - PP_5167 0.26 +0.6 5,892,840 - PP_5167 0.26 +0.1 5,893,006 + PP_5167 0.55 +0.9 5,893,007 - PP_5167 0.55 +1.3 5,893,248 - +0.9 5,893,248 - +0.0 5,893,284 + -0.2 5,893,284 + +2.3 5,893,308 + +3.3 5,893,308 + +0.2 5,893,308 + -0.2 5,893,308 + -0.1 5,893,308 + +4.3 5,893,308 + -2.4 5,893,308 + +1.5 5,893,308 + -2.3 5,893,308 + +0.1 5,893,308 + -2.1 5,893,308 + +0.5 5,893,309 - -0.5 5,893,309 - +0.7 5,893,309 - -1.7 5,893,309 - +3.9 5,893,309 - -2.1 5,893,309 - -3.2 5,893,309 - +1.6 5,893,309 - +0.1 5,893,309 - +3.7 5,893,309 - -2.8 5,893,309 - +3.9 5,893,309 - +2.3 5,893,309 - -2.2 5,893,309 - -0.3 5,893,310 + +2.0 5,893,310 + +1.1 5,893,311 - +0.9 5,893,311 - +0.2 5,893,407 + -0.0 5,893,407 + +0.8 5,893,408 - -0.7 5,893,408 - -1.1 5,893,481 + -2.0 5,893,481 + +0.6 5,893,481 + +0.7 5,893,481 + +2.6 5,893,481 + +2.7 5,893,481 + +0.8 5,893,482 - -0.6 5,893,482 - -0.8 5,893,482 - -1.3 5,893,482 - +1.3 5,893,482 - +1.2 5,893,482 - +0.0 5,893,482 - -0.2 5,893,485 + -0.3 5,893,486 - +3.3
Or see this region's nucleotide sequence