Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5078 and PP_5079 are separated by 96 nucleotides PP_5079 and PP_5080 are separated by 4 nucleotides PP_5080 and PP_5081 overlap by 53 nucleotides PP_5081 and PP_5082 overlap by 4 nucleotides
PP_5078: PP_5078 - 3-dehydroquinate synthase, at 5,799,878 to 5,800,975
_5078
PP_5079: PP_5079 - Shikimate kinase, at 5,801,072 to 5,801,590
_5079
PP_5080: PP_5080 - type 4 pili biogenesis protein, at 5,801,595 to 5,802,833
_5080
PP_5081: PP_5081 - Type IV pili biogenesis protein, at 5,802,781 to 5,803,797
_5081
PP_5082: PP_5082 - Type IV pili biogenesis protein PilN, at 5,803,794 to 5,804,330
_5082
Position (kb) 5801
5802
5803 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5800.965 kb on - strand at 5800.978 kb on - strand at 5801.062 kb on + strand at 5801.062 kb on + strand at 5801.674 kb on - strand at 5801.704 kb on + strand at 5801.704 kb on + strand at 5801.705 kb on - strand at 5801.705 kb on - strand at 5801.838 kb on + strand, within PP_5080 at 5801.839 kb on - strand, within PP_5080 at 5801.928 kb on + strand, within PP_5080 at 5801.929 kb on - strand, within PP_5080 at 5801.995 kb on + strand, within PP_5080 at 5802.215 kb on - strand, within PP_5080 at 5802.254 kb on - strand, within PP_5080 at 5802.275 kb on + strand, within PP_5080 at 5802.685 kb on - strand, within PP_5080 at 5802.685 kb on - strand, within PP_5080 at 5802.685 kb on - strand, within PP_5080 at 5802.718 kb on + strand at 5802.719 kb on - strand at 5802.838 kb on + strand at 5802.839 kb on - strand at 5802.839 kb on - strand at 5802.839 kb on - strand at 5802.970 kb on + strand, within PP_5081 at 5802.970 kb on + strand, within PP_5081 at 5802.970 kb on + strand, within PP_5081 at 5802.970 kb on + strand, within PP_5081 at 5802.970 kb on + strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5803.230 kb on + strand, within PP_5081 at 5803.231 kb on - strand, within PP_5081 at 5803.399 kb on + strand, within PP_5081 at 5803.435 kb on + strand, within PP_5081 at 5803.435 kb on + strand, within PP_5081 at 5803.436 kb on - strand, within PP_5081 at 5803.436 kb on - strand, within PP_5081 at 5803.526 kb on + strand, within PP_5081 at 5803.526 kb on + strand, within PP_5081 at 5803.567 kb on + strand, within PP_5081 at 5803.568 kb on - strand, within PP_5081 at 5803.568 kb on - strand, within PP_5081 at 5803.666 kb on + strand, within PP_5081 at 5803.666 kb on + strand, within PP_5081 at 5803.667 kb on - strand, within PP_5081
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 5,800,965 - +0.8 5,800,978 - +0.8 5,801,062 + +0.8 5,801,062 + +0.8 5,801,674 - +0.3 5,801,704 + -2.4 5,801,704 + -1.6 5,801,705 - +0.0 5,801,705 - +1.0 5,801,838 + PP_5080 0.20 +0.8 5,801,839 - PP_5080 0.20 -1.4 5,801,928 + PP_5080 0.27 -2.5 5,801,929 - PP_5080 0.27 -2.2 5,801,995 + PP_5080 0.32 -0.8 5,802,215 - PP_5080 0.50 +0.8 5,802,254 - PP_5080 0.53 -3.3 5,802,275 + PP_5080 0.55 +1.4 5,802,685 - PP_5080 0.88 -0.5 5,802,685 - PP_5080 0.88 -1.6 5,802,685 - PP_5080 0.88 -2.2 5,802,718 + +3.8 5,802,719 - +0.0 5,802,838 + +0.4 5,802,839 - +2.1 5,802,839 - -1.0 5,802,839 - +0.0 5,802,970 + PP_5081 0.19 -0.8 5,802,970 + PP_5081 0.19 -3.3 5,802,970 + PP_5081 0.19 +0.4 5,802,970 + PP_5081 0.19 -1.8 5,802,970 + PP_5081 0.19 +0.9 5,802,971 - PP_5081 0.19 -1.8 5,802,971 - PP_5081 0.19 -1.2 5,802,971 - PP_5081 0.19 -1.6 5,802,971 - PP_5081 0.19 -0.0 5,802,971 - PP_5081 0.19 -0.9 5,802,971 - PP_5081 0.19 -2.2 5,802,971 - PP_5081 0.19 +3.8 5,802,971 - PP_5081 0.19 -1.5 5,802,971 - PP_5081 0.19 +0.3 5,802,971 - PP_5081 0.19 +0.8 5,802,971 - PP_5081 0.19 -4.5 5,802,972 + PP_5081 0.19 -0.8 5,802,972 + PP_5081 0.19 -0.1 5,802,972 + PP_5081 0.19 +0.5 5,802,972 + PP_5081 0.19 +0.3 5,802,972 + PP_5081 0.19 -2.1 5,802,973 - PP_5081 0.19 +1.1 5,802,973 - PP_5081 0.19 -0.7 5,802,973 - PP_5081 0.19 +0.9 5,802,973 - PP_5081 0.19 -0.6 5,802,973 - PP_5081 0.19 -1.6 5,803,230 + PP_5081 0.44 +0.8 5,803,231 - PP_5081 0.44 -0.1 5,803,399 + PP_5081 0.61 +1.8 5,803,435 + PP_5081 0.64 -0.2 5,803,435 + PP_5081 0.64 +0.6 5,803,436 - PP_5081 0.64 -1.3 5,803,436 - PP_5081 0.64 -0.6 5,803,526 + PP_5081 0.73 -0.1 5,803,526 + PP_5081 0.73 -3.0 5,803,567 + PP_5081 0.77 -2.1 5,803,568 - PP_5081 0.77 -0.2 5,803,568 - PP_5081 0.77 +0.3 5,803,666 + PP_5081 0.87 +0.2 5,803,666 + PP_5081 0.87 -0.7 5,803,667 - PP_5081 0.87 +1.8
Or see this region's nucleotide sequence