Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4848 and PP_4849 are separated by 164 nucleotides PP_4849 and PP_4850 are separated by 51 nucleotides
PP_4848: PP_4848 - Curved DNA-binding protein, at 5,515,066 to 5,516,025
_4848
PP_4849: PP_4849 - putative DnaK protein, at 5,516,190 to 5,517,461
_4849
PP_4850: PP_4850 - conserved membrane protein of unknown function, at 5,517,513 to 5,518,580
_4850
Position (kb)
5516
5517
5518 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5515.814 kb on + strand, within PP_4848 at 5515.815 kb on - strand, within PP_4848 at 5515.868 kb on + strand, within PP_4848 at 5515.869 kb on - strand, within PP_4848 at 5515.931 kb on + strand at 5515.931 kb on + strand at 5515.932 kb on - strand at 5515.932 kb on - strand at 5515.999 kb on + strand at 5516.006 kb on + strand at 5516.006 kb on + strand at 5516.007 kb on - strand at 5516.007 kb on - strand at 5516.007 kb on - strand at 5516.007 kb on - strand at 5516.009 kb on + strand at 5516.009 kb on + strand at 5516.010 kb on - strand at 5516.028 kb on - strand at 5516.028 kb on - strand at 5516.080 kb on + strand at 5516.080 kb on + strand at 5516.080 kb on + strand at 5516.080 kb on + strand at 5516.150 kb on + strand at 5516.150 kb on + strand at 5516.150 kb on + strand at 5516.151 kb on - strand at 5516.151 kb on - strand at 5516.151 kb on - strand at 5516.151 kb on - strand at 5516.151 kb on - strand at 5516.201 kb on - strand at 5516.355 kb on + strand, within PP_4849 at 5516.355 kb on + strand, within PP_4849 at 5516.676 kb on + strand, within PP_4849 at 5516.676 kb on + strand, within PP_4849 at 5516.677 kb on - strand, within PP_4849 at 5516.677 kb on - strand, within PP_4849 at 5516.703 kb on + strand, within PP_4849 at 5516.734 kb on + strand, within PP_4849 at 5516.910 kb on + strand, within PP_4849 at 5516.911 kb on - strand, within PP_4849 at 5516.952 kb on + strand, within PP_4849 at 5516.952 kb on + strand, within PP_4849 at 5516.953 kb on - strand, within PP_4849 at 5516.953 kb on - strand, within PP_4849 at 5516.995 kb on - strand, within PP_4849 at 5516.995 kb on - strand, within PP_4849 at 5517.034 kb on - strand, within PP_4849 at 5517.305 kb on + strand, within PP_4849 at 5517.398 kb on + strand at 5517.398 kb on + strand at 5517.399 kb on - strand at 5517.506 kb on + strand at 5517.506 kb on + strand at 5517.506 kb on + strand at 5517.506 kb on + strand at 5517.507 kb on - strand at 5517.507 kb on - strand at 5517.507 kb on - strand at 5517.507 kb on - strand at 5517.550 kb on + strand at 5517.551 kb on - strand at 5517.556 kb on + strand at 5517.556 kb on + strand at 5517.556 kb on + strand at 5517.556 kb on + strand at 5517.556 kb on + strand at 5517.556 kb on + strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.557 kb on - strand at 5517.559 kb on + strand at 5517.559 kb on + strand at 5517.559 kb on + strand at 5517.559 kb on + strand at 5517.559 kb on + strand at 5517.559 kb on + strand at 5517.559 kb on + strand at 5517.559 kb on + strand at 5517.559 kb on + strand at 5517.560 kb on - strand at 5517.560 kb on - strand at 5517.560 kb on - strand at 5517.560 kb on - strand at 5517.560 kb on - strand at 5517.560 kb on - strand at 5517.560 kb on - strand at 5517.560 kb on - strand at 5517.606 kb on + strand at 5517.607 kb on - strand at 5517.679 kb on + strand, within PP_4850 at 5517.679 kb on + strand, within PP_4850 at 5517.849 kb on + strand, within PP_4850 at 5517.850 kb on - strand, within PP_4850 at 5517.850 kb on - strand, within PP_4850 at 5517.850 kb on - strand, within PP_4850 at 5518.171 kb on + strand, within PP_4850 at 5518.171 kb on + strand, within PP_4850
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 5,515,814 + PP_4848 0.78 +1.7 5,515,815 - PP_4848 0.78 +1.7 5,515,868 + PP_4848 0.84 -0.6 5,515,869 - PP_4848 0.84 +0.7 5,515,931 + +1.0 5,515,931 + +0.2 5,515,932 - -0.5 5,515,932 - +1.7 5,515,999 + -0.6 5,516,006 + -0.9 5,516,006 + -0.7 5,516,007 - +1.6 5,516,007 - +0.2 5,516,007 - -1.3 5,516,007 - +2.3 5,516,009 + -1.0 5,516,009 + -0.5 5,516,010 - -0.1 5,516,028 - -0.2 5,516,028 - -2.6 5,516,080 + -3.5 5,516,080 + +1.3 5,516,080 + -0.9 5,516,080 + -1.3 5,516,150 + +1.2 5,516,150 + +2.1 5,516,150 + +0.5 5,516,151 - +1.4 5,516,151 - +3.5 5,516,151 - +0.7 5,516,151 - +0.7 5,516,151 - +0.6 5,516,201 - -0.3 5,516,355 + PP_4849 0.13 -1.3 5,516,355 + PP_4849 0.13 -1.3 5,516,676 + PP_4849 0.38 -2.3 5,516,676 + PP_4849 0.38 -2.6 5,516,677 - PP_4849 0.38 +0.1 5,516,677 - PP_4849 0.38 -0.7 5,516,703 + PP_4849 0.40 +3.3 5,516,734 + PP_4849 0.43 -2.1 5,516,910 + PP_4849 0.57 -0.3 5,516,911 - PP_4849 0.57 -0.3 5,516,952 + PP_4849 0.60 +0.8 5,516,952 + PP_4849 0.60 +0.3 5,516,953 - PP_4849 0.60 -1.1 5,516,953 - PP_4849 0.60 -1.3 5,516,995 - PP_4849 0.63 +3.3 5,516,995 - PP_4849 0.63 -0.9 5,517,034 - PP_4849 0.66 -1.8 5,517,305 + PP_4849 0.88 -0.3 5,517,398 + -1.2 5,517,398 + +0.9 5,517,399 - +0.7 5,517,506 + -2.3 5,517,506 + +0.4 5,517,506 + -0.4 5,517,506 + -1.8 5,517,507 - +0.1 5,517,507 - +0.3 5,517,507 - -0.6 5,517,507 - +3.6 5,517,550 + -1.6 5,517,551 - -0.3 5,517,556 + -1.5 5,517,556 + -1.0 5,517,556 + -0.3 5,517,556 + -1.3 5,517,556 + +1.7 5,517,556 + -1.8 5,517,557 - +1.1 5,517,557 - +0.3 5,517,557 - +0.9 5,517,557 - -2.6 5,517,557 - +2.2 5,517,557 - -1.8 5,517,557 - +0.3 5,517,557 - -0.0 5,517,557 - +1.5 5,517,557 - -3.5 5,517,559 + +0.7 5,517,559 + -0.4 5,517,559 + -0.9 5,517,559 + -0.5 5,517,559 + +0.6 5,517,559 + -3.4 5,517,559 + -3.3 5,517,559 + +3.7 5,517,559 + -1.4 5,517,560 - -4.0 5,517,560 - +0.2 5,517,560 - -2.8 5,517,560 - -0.3 5,517,560 - -0.3 5,517,560 - +0.7 5,517,560 - +0.2 5,517,560 - +2.7 5,517,606 + -1.1 5,517,607 - +0.3 5,517,679 + PP_4850 0.16 +0.2 5,517,679 + PP_4850 0.16 +0.1 5,517,849 + PP_4850 0.31 -2.6 5,517,850 - PP_4850 0.32 +1.2 5,517,850 - PP_4850 0.32 +0.6 5,517,850 - PP_4850 0.32 +0.6 5,518,171 + PP_4850 0.62 +0.7 5,518,171 + PP_4850 0.62 +0.6
Or see this region's nucleotide sequence