Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3280 and PP_3281 overlap by 8 nucleotides PP_3281 and PP_3282 overlap by 8 nucleotides PP_3282 and PP_3283 are separated by 2 nucleotides PP_3283 and PP_3284 are separated by 34 nucleotides
PP_3280: PP_3280 - 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase, at 3,714,635 to 3,715,855
_3280
PP_3281: PP_3281 - hydroxyphenylacetyl-CoA thioesterase, at 3,715,848 to 3,716,288
_3281
PP_3282: PP_3282 - 3-hydroxyadipyl-CoA dehydrogenase, at 3,716,281 to 3,717,798
_3282
PP_3283: PP_3283 - 1,2-epoxyphenylacetyl-CoA isomerase, at 3,717,801 to 3,718,592
_3283
PP_3284: PP_3284 - enoyl-CoA hydratase-isomerase, at 3,718,627 to 3,719,400
_3284
Position (kb)
3716
3717
3718 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3715.457 kb on - strand, within PP_3280 at 3715.599 kb on - strand, within PP_3280 at 3715.684 kb on + strand, within PP_3280 at 3715.684 kb on + strand, within PP_3280 at 3715.684 kb on + strand, within PP_3280 at 3715.685 kb on - strand, within PP_3280 at 3715.895 kb on - strand, within PP_3281 at 3715.954 kb on + strand, within PP_3281 at 3715.955 kb on - strand, within PP_3281 at 3715.955 kb on - strand, within PP_3281 at 3716.032 kb on + strand, within PP_3281 at 3716.033 kb on - strand, within PP_3281 at 3716.072 kb on - strand, within PP_3281 at 3716.072 kb on - strand, within PP_3281 at 3716.075 kb on + strand, within PP_3281 at 3716.075 kb on + strand, within PP_3281 at 3716.075 kb on + strand, within PP_3281 at 3716.076 kb on - strand, within PP_3281 at 3716.164 kb on - strand, within PP_3281 at 3716.290 kb on + strand at 3716.290 kb on + strand at 3716.291 kb on - strand at 3716.291 kb on - strand at 3716.291 kb on - strand at 3716.291 kb on - strand at 3716.436 kb on - strand, within PP_3282 at 3716.673 kb on - strand, within PP_3282 at 3716.940 kb on - strand, within PP_3282 at 3716.945 kb on + strand, within PP_3282 at 3716.945 kb on + strand, within PP_3282 at 3716.946 kb on - strand, within PP_3282 at 3716.946 kb on - strand, within PP_3282 at 3716.946 kb on - strand, within PP_3282 at 3717.047 kb on - strand, within PP_3282 at 3717.065 kb on + strand, within PP_3282 at 3717.065 kb on + strand, within PP_3282 at 3717.065 kb on + strand, within PP_3282 at 3717.065 kb on + strand, within PP_3282 at 3717.065 kb on + strand, within PP_3282 at 3717.066 kb on - strand, within PP_3282 at 3717.066 kb on - strand, within PP_3282 at 3717.066 kb on - strand, within PP_3282 at 3717.066 kb on - strand, within PP_3282 at 3717.184 kb on + strand, within PP_3282 at 3717.185 kb on - strand, within PP_3282 at 3717.198 kb on - strand, within PP_3282 at 3717.198 kb on - strand at 3717.238 kb on - strand, within PP_3282 at 3717.284 kb on + strand, within PP_3282 at 3717.285 kb on - strand, within PP_3282 at 3717.285 kb on - strand, within PP_3282 at 3717.372 kb on + strand, within PP_3282 at 3717.435 kb on + strand, within PP_3282 at 3717.435 kb on + strand, within PP_3282 at 3717.436 kb on - strand, within PP_3282 at 3717.451 kb on - strand, within PP_3282 at 3717.451 kb on - strand, within PP_3282 at 3717.451 kb on - strand, within PP_3282 at 3717.451 kb on - strand, within PP_3282 at 3717.610 kb on + strand, within PP_3282 at 3717.692 kb on + strand at 3717.970 kb on + strand, within PP_3283 at 3717.971 kb on - strand, within PP_3283 at 3717.971 kb on - strand, within PP_3283 at 3718.148 kb on + strand, within PP_3283 at 3718.148 kb on + strand, within PP_3283 at 3718.281 kb on + strand, within PP_3283 at 3718.281 kb on + strand, within PP_3283 at 3718.281 kb on + strand, within PP_3283 at 3718.327 kb on + strand, within PP_3283 at 3718.328 kb on - strand, within PP_3283 at 3718.506 kb on - strand, within PP_3283 at 3718.506 kb on - strand, within PP_3283
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 3,715,457 - PP_3280 0.67 -0.7 3,715,599 - PP_3280 0.79 -1.6 3,715,684 + PP_3280 0.86 +0.7 3,715,684 + PP_3280 0.86 -0.8 3,715,684 + PP_3280 0.86 -0.3 3,715,685 - PP_3280 0.86 -1.4 3,715,895 - PP_3281 0.11 -0.3 3,715,954 + PP_3281 0.24 +1.0 3,715,955 - PP_3281 0.24 -1.3 3,715,955 - PP_3281 0.24 +1.4 3,716,032 + PP_3281 0.42 -3.3 3,716,033 - PP_3281 0.42 -1.6 3,716,072 - PP_3281 0.51 -1.3 3,716,072 - PP_3281 0.51 +3.7 3,716,075 + PP_3281 0.51 +0.6 3,716,075 + PP_3281 0.51 -0.3 3,716,075 + PP_3281 0.51 -3.0 3,716,076 - PP_3281 0.52 -0.5 3,716,164 - PP_3281 0.72 -0.7 3,716,290 + -2.3 3,716,290 + +1.2 3,716,291 - -2.1 3,716,291 - -1.0 3,716,291 - +1.8 3,716,291 - -1.3 3,716,436 - PP_3282 0.10 -1.1 3,716,673 - PP_3282 0.26 -2.1 3,716,940 - PP_3282 0.43 +1.2 3,716,945 + PP_3282 0.44 -2.4 3,716,945 + PP_3282 0.44 +0.3 3,716,946 - PP_3282 0.44 +1.2 3,716,946 - PP_3282 0.44 -1.3 3,716,946 - PP_3282 0.44 +1.7 3,717,047 - PP_3282 0.50 -0.3 3,717,065 + PP_3282 0.52 +0.1 3,717,065 + PP_3282 0.52 +0.2 3,717,065 + PP_3282 0.52 -0.6 3,717,065 + PP_3282 0.52 +2.3 3,717,065 + PP_3282 0.52 +0.1 3,717,066 - PP_3282 0.52 -0.2 3,717,066 - PP_3282 0.52 -0.1 3,717,066 - PP_3282 0.52 +0.4 3,717,066 - PP_3282 0.52 -1.7 3,717,184 + PP_3282 0.59 -1.4 3,717,185 - PP_3282 0.60 -0.3 3,717,198 - PP_3282 0.60 -1.4 3,717,198 - +2.5 3,717,238 - PP_3282 0.63 +0.2 3,717,284 + PP_3282 0.66 +3.1 3,717,285 - PP_3282 0.66 +1.6 3,717,285 - PP_3282 0.66 -2.4 3,717,372 + PP_3282 0.72 -2.1 3,717,435 + PP_3282 0.76 +0.7 3,717,435 + PP_3282 0.76 +3.3 3,717,436 - PP_3282 0.76 -1.3 3,717,451 - PP_3282 0.77 -0.0 3,717,451 - PP_3282 0.77 +0.7 3,717,451 - PP_3282 0.77 -2.7 3,717,451 - PP_3282 0.77 +1.0 3,717,610 + PP_3282 0.88 +1.7 3,717,692 + -0.3 3,717,970 + PP_3283 0.21 +0.3 3,717,971 - PP_3283 0.21 -0.2 3,717,971 - PP_3283 0.21 +0.7 3,718,148 + PP_3283 0.44 +1.1 3,718,148 + PP_3283 0.44 +1.0 3,718,281 + PP_3283 0.61 +1.2 3,718,281 + PP_3283 0.61 +2.7 3,718,281 + PP_3283 0.61 -1.2 3,718,327 + PP_3283 0.66 -0.1 3,718,328 - PP_3283 0.67 +1.3 3,718,506 - PP_3283 0.89 +1.5 3,718,506 - PP_3283 0.89 +1.9
Or see this region's nucleotide sequence