Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3192 and PP_3193 are separated by 225 nucleotides PP_3193 and PP_3194 overlap by 13 nucleotides PP_3194 and PP_3195 are separated by 172 nucleotides
PP_3192: PP_3192 - conserved protein of unknown function, at 3,621,432 to 3,622,037
_3192
PP_3193: PP_3193 - conserved protein of unknown function, at 3,622,263 to 3,622,721
_3193
PP_3194: PP_3194 - conserved protein of unknown function, at 3,622,709 to 3,624,052
_3194
PP_3195: PP_3195 - conserved protein of unknown function, at 3,624,225 to 3,625,487
_3195
Position (kb)
3622
3623
3624
3625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3621.760 kb on + strand, within PP_3192 at 3621.855 kb on - strand, within PP_3192 at 3621.923 kb on + strand, within PP_3192 at 3621.933 kb on - strand, within PP_3192 at 3621.955 kb on - strand, within PP_3192 at 3621.985 kb on + strand at 3622.043 kb on - strand at 3622.069 kb on - strand at 3622.069 kb on - strand at 3622.087 kb on - strand at 3622.157 kb on - strand at 3622.626 kb on - strand, within PP_3193 at 3622.752 kb on + strand at 3622.753 kb on - strand at 3622.804 kb on - strand at 3622.842 kb on + strand at 3622.842 kb on + strand at 3622.842 kb on + strand at 3622.843 kb on - strand at 3623.030 kb on + strand, within PP_3194 at 3623.112 kb on - strand, within PP_3194 at 3623.195 kb on - strand, within PP_3194 at 3623.305 kb on - strand, within PP_3194 at 3623.409 kb on + strand, within PP_3194 at 3623.578 kb on - strand, within PP_3194 at 3624.089 kb on - strand at 3624.122 kb on + strand at 3624.123 kb on - strand at 3624.123 kb on - strand at 3624.271 kb on + strand at 3624.271 kb on + strand at 3624.272 kb on - strand at 3624.272 kb on - strand at 3624.442 kb on - strand, within PP_3195 at 3624.474 kb on + strand, within PP_3195 at 3624.670 kb on + strand, within PP_3195 at 3624.747 kb on + strand, within PP_3195
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 3,621,760 + PP_3192 0.54 +2.3 3,621,855 - PP_3192 0.70 +0.5 3,621,923 + PP_3192 0.81 +1.0 3,621,933 - PP_3192 0.83 +3.1 3,621,955 - PP_3192 0.86 -0.2 3,621,985 + -0.6 3,622,043 - +0.2 3,622,069 - +1.2 3,622,069 - -1.1 3,622,087 - +2.8 3,622,157 - +0.8 3,622,626 - PP_3193 0.79 +3.3 3,622,752 + -0.2 3,622,753 - -0.8 3,622,804 - -1.7 3,622,842 + -2.2 3,622,842 + -3.1 3,622,842 + +0.8 3,622,843 - +0.4 3,623,030 + PP_3194 0.24 +0.5 3,623,112 - PP_3194 0.30 -1.9 3,623,195 - PP_3194 0.36 +0.9 3,623,305 - PP_3194 0.44 +0.1 3,623,409 + PP_3194 0.52 +1.1 3,623,578 - PP_3194 0.65 +2.7 3,624,089 - -0.7 3,624,122 + +0.3 3,624,123 - -0.4 3,624,123 - +0.1 3,624,271 + +0.8 3,624,271 + +0.5 3,624,272 - -1.8 3,624,272 - -1.0 3,624,442 - PP_3195 0.17 -1.5 3,624,474 + PP_3195 0.20 +1.3 3,624,670 + PP_3195 0.35 -0.9 3,624,747 + PP_3195 0.41 +0.8
Or see this region's nucleotide sequence