Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3191 and PP_3192 are separated by 30 nucleotides PP_3192 and PP_3193 are separated by 225 nucleotides PP_3193 and PP_3194 overlap by 13 nucleotides
PP_3191: PP_3191 - putative threonine ammonia-lyase / dehydratase, at 3,620,349 to 3,621,401
_3191
PP_3192: PP_3192 - conserved protein of unknown function, at 3,621,432 to 3,622,037
_3192
PP_3193: PP_3193 - conserved protein of unknown function, at 3,622,263 to 3,622,721
_3193
PP_3194: PP_3194 - conserved protein of unknown function, at 3,622,709 to 3,624,052
_3194
Position (kb)
3621
3622
3623 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3620.549 kb on + strand, within PP_3191 at 3620.829 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.866 kb on + strand, within PP_3191 at 3620.883 kb on + strand, within PP_3191 at 3621.013 kb on + strand, within PP_3191 at 3621.014 kb on - strand, within PP_3191 at 3621.014 kb on - strand, within PP_3191 at 3621.299 kb on + strand at 3621.300 kb on - strand at 3621.300 kb on - strand at 3621.300 kb on - strand at 3621.454 kb on + strand at 3621.455 kb on - strand at 3621.460 kb on + strand at 3621.500 kb on - strand, within PP_3192 at 3621.596 kb on + strand, within PP_3192 at 3621.760 kb on + strand, within PP_3192 at 3621.855 kb on - strand, within PP_3192 at 3621.923 kb on + strand, within PP_3192 at 3621.933 kb on - strand, within PP_3192 at 3621.955 kb on - strand, within PP_3192 at 3621.985 kb on + strand at 3622.043 kb on - strand at 3622.069 kb on - strand at 3622.069 kb on - strand at 3622.087 kb on - strand at 3622.157 kb on - strand at 3622.626 kb on - strand, within PP_3193 at 3622.752 kb on + strand at 3622.753 kb on - strand at 3622.804 kb on - strand at 3622.842 kb on + strand at 3622.842 kb on + strand at 3622.842 kb on + strand at 3622.843 kb on - strand at 3623.030 kb on + strand, within PP_3194
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 3,620,549 + PP_3191 0.19 -0.7 3,620,829 + PP_3191 0.46 -1.2 3,620,866 + PP_3191 0.49 +1.8 3,620,866 + PP_3191 0.49 -1.9 3,620,866 + PP_3191 0.49 -1.6 3,620,883 + PP_3191 0.51 -0.4 3,621,013 + PP_3191 0.63 -0.7 3,621,014 - PP_3191 0.63 -1.3 3,621,014 - PP_3191 0.63 -0.5 3,621,299 + +0.1 3,621,300 - +1.9 3,621,300 - -1.0 3,621,300 - +1.4 3,621,454 + +2.9 3,621,455 - +3.1 3,621,460 + -2.2 3,621,500 - PP_3192 0.11 +2.0 3,621,596 + PP_3192 0.27 +1.1 3,621,760 + PP_3192 0.54 +2.3 3,621,855 - PP_3192 0.70 +0.5 3,621,923 + PP_3192 0.81 +1.0 3,621,933 - PP_3192 0.83 +3.1 3,621,955 - PP_3192 0.86 -0.2 3,621,985 + -0.6 3,622,043 - +0.2 3,622,069 - +1.2 3,622,069 - -1.1 3,622,087 - +2.8 3,622,157 - +0.8 3,622,626 - PP_3193 0.79 +3.3 3,622,752 + -0.2 3,622,753 - -0.8 3,622,804 - -1.7 3,622,842 + -2.2 3,622,842 + -3.1 3,622,842 + +0.8 3,622,843 - +0.4 3,623,030 + PP_3194 0.24 +0.5
Or see this region's nucleotide sequence