Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3032 and PP_5552 are separated by 3 nucleotides PP_5552 and PP_5553 overlap by 4 nucleotides PP_5553 and PP_3033 are separated by 107 nucleotides PP_3033 and PP_3034 are separated by 78 nucleotides PP_3034 and PP_3035 overlap by 90 nucleotides PP_3035 and PP_5554 are separated by 108 nucleotides PP_5554 and PP_3036 are separated by 71 nucleotides PP_3036 and PP_5555 overlap by 29 nucleotides
PP_3032: PP_3032 - DNA-binding protein Roi-related protein, at 3,418,570 to 3,418,860
_3032
PP_5552: PP_5552 - conserved protein of unknown function, at 3,418,864 to 3,419,175
_5552
PP_5553: PP_5553 - conserved protein of unknown function, at 3,419,172 to 3,419,345
_5553
PP_3033: PP_3033 - putative transcriptional repressor, CI/C2 family, at 3,419,453 to 3,420,184
_3033
PP_3034: PP_3034 - putative Regulator protein, at 3,420,263 to 3,420,526
_3034
PP_3035: PP_3035 - conserved protein of unknown function, at 3,420,437 to 3,420,610
_3035
PP_5554: PP_5554 - protein of unknown function, at 3,420,719 to 3,420,901
_5554
PP_3036: PP_3036 - conserved protein of unknown function, at 3,420,973 to 3,421,152
_3036
PP_5555: PP_5555 - conserved protein of unknown function, at 3,421,124 to 3,421,642
_5555
Position (kb)
3419
3420
3421 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3418.580 kb on + strand at 3418.673 kb on + strand, within PP_3032 at 3418.865 kb on + strand at 3418.880 kb on - strand at 3418.880 kb on - strand at 3418.882 kb on + strand at 3418.883 kb on - strand at 3418.883 kb on - strand at 3418.932 kb on + strand, within PP_5552 at 3419.137 kb on - strand, within PP_5552 at 3419.168 kb on + strand at 3419.195 kb on - strand, within PP_5553 at 3419.195 kb on - strand, within PP_5553 at 3419.228 kb on + strand, within PP_5553 at 3419.229 kb on - strand, within PP_5553 at 3419.241 kb on - strand, within PP_5553 at 3419.241 kb on - strand, within PP_5553 at 3419.241 kb on - strand, within PP_5553 at 3419.292 kb on + strand, within PP_5553 at 3419.325 kb on + strand, within PP_5553 at 3419.325 kb on + strand, within PP_5553 at 3419.325 kb on + strand, within PP_5553 at 3419.325 kb on + strand at 3419.325 kb on + strand, within PP_5553 at 3419.326 kb on - strand, within PP_5553 at 3419.326 kb on - strand at 3419.326 kb on - strand, within PP_5553 at 3419.326 kb on - strand, within PP_5553 at 3419.326 kb on - strand, within PP_5553 at 3419.348 kb on + strand at 3419.348 kb on + strand at 3420.352 kb on - strand, within PP_3034 at 3420.357 kb on - strand at 3420.357 kb on - strand, within PP_3034 at 3420.398 kb on - strand, within PP_3034 at 3420.408 kb on - strand, within PP_3034 at 3420.525 kb on - strand at 3420.565 kb on - strand, within PP_3035 at 3420.565 kb on - strand, within PP_3035 at 3420.727 kb on - strand at 3420.730 kb on - strand at 3420.730 kb on - strand at 3420.758 kb on - strand, within PP_5554 at 3420.791 kb on - strand, within PP_5554 at 3420.791 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.881 kb on - strand, within PP_5554 at 3420.885 kb on - strand at 3420.902 kb on - strand at 3420.902 kb on - strand at 3420.902 kb on - strand at 3420.902 kb on - strand at 3420.902 kb on - strand at 3420.933 kb on + strand at 3420.977 kb on - strand at 3421.054 kb on + strand, within PP_3036 at 3421.055 kb on - strand, within PP_3036 at 3421.055 kb on - strand, within PP_3036 at 3421.055 kb on - strand, within PP_3036 at 3421.055 kb on - strand, within PP_3036 at 3421.062 kb on + strand, within PP_3036 at 3421.063 kb on - strand, within PP_3036
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 3,418,580 + +0.5 3,418,673 + PP_3032 0.35 -1.0 3,418,865 + -0.5 3,418,880 - -0.9 3,418,880 - -1.5 3,418,882 + -0.5 3,418,883 - -1.8 3,418,883 - -0.8 3,418,932 + PP_5552 0.22 -0.2 3,419,137 - PP_5552 0.88 +3.4 3,419,168 + -1.3 3,419,195 - PP_5553 0.13 -1.1 3,419,195 - PP_5553 0.13 +3.9 3,419,228 + PP_5553 0.32 -1.0 3,419,229 - PP_5553 0.33 -1.0 3,419,241 - PP_5553 0.40 +0.2 3,419,241 - PP_5553 0.40 -4.6 3,419,241 - PP_5553 0.40 +0.8 3,419,292 + PP_5553 0.69 -0.1 3,419,325 + PP_5553 0.88 +1.5 3,419,325 + PP_5553 0.88 -0.3 3,419,325 + PP_5553 0.88 -1.4 3,419,325 + +1.4 3,419,325 + PP_5553 0.88 -0.5 3,419,326 - PP_5553 0.89 -1.4 3,419,326 - +0.8 3,419,326 - PP_5553 0.89 -3.9 3,419,326 - PP_5553 0.89 -0.8 3,419,326 - PP_5553 0.89 +0.1 3,419,348 + +0.1 3,419,348 + -1.4 3,420,352 - PP_3034 0.34 -3.2 3,420,357 - -0.8 3,420,357 - PP_3034 0.36 -0.4 3,420,398 - PP_3034 0.51 -1.0 3,420,408 - PP_3034 0.55 -1.8 3,420,525 - -1.5 3,420,565 - PP_3035 0.74 -2.4 3,420,565 - PP_3035 0.74 -2.4 3,420,727 - -1.0 3,420,730 - +1.6 3,420,730 - -3.2 3,420,758 - PP_5554 0.21 +0.8 3,420,791 - PP_5554 0.39 -2.5 3,420,791 - PP_5554 0.39 +1.8 3,420,800 - PP_5554 0.44 -1.1 3,420,800 - PP_5554 0.44 -0.4 3,420,800 - PP_5554 0.44 +2.8 3,420,800 - PP_5554 0.44 +0.8 3,420,800 - PP_5554 0.44 -1.5 3,420,800 - PP_5554 0.44 -1.2 3,420,800 - PP_5554 0.44 +0.0 3,420,800 - PP_5554 0.44 -2.2 3,420,881 - PP_5554 0.89 +0.8 3,420,885 - +1.0 3,420,902 - +0.9 3,420,902 - -0.0 3,420,902 - -0.4 3,420,902 - -0.2 3,420,902 - -0.0 3,420,933 + +1.7 3,420,977 - -0.1 3,421,054 + PP_3036 0.45 -0.5 3,421,055 - PP_3036 0.46 +3.6 3,421,055 - PP_3036 0.46 +0.9 3,421,055 - PP_3036 0.46 -0.8 3,421,055 - PP_3036 0.46 +0.9 3,421,062 + PP_3036 0.49 -0.9 3,421,063 - PP_3036 0.50 -2.5
Or see this region's nucleotide sequence