Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1513 and PP_1514 are separated by 192 nucleotides PP_1514 and PP_1515 are separated by 129 nucleotides
PP_1513: PP_1513 - Ribosomal RNA small subunit methyltransferase J, at 1,717,302 to 1,718,078
_1513
PP_1514: PP_1514 - conserved protein of unknown function, at 1,718,271 to 1,719,239
_1514
PP_1515: PP_1515 - Transcriptional regulator, tetR family, at 1,719,369 to 1,720,010
_1515
Position (kb)
1718
1719
1720 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1717.351 kb on - strand at 1717.351 kb on - strand at 1717.594 kb on + strand, within PP_1513 at 1717.825 kb on - strand, within PP_1513 at 1717.825 kb on - strand, within PP_1513 at 1718.019 kb on - strand at 1718.031 kb on - strand at 1718.044 kb on - strand at 1718.052 kb on + strand at 1718.052 kb on + strand at 1718.053 kb on - strand at 1718.087 kb on + strand at 1718.088 kb on - strand at 1718.110 kb on + strand at 1718.110 kb on + strand at 1718.110 kb on + strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.176 kb on + strand at 1718.188 kb on + strand at 1718.188 kb on + strand at 1718.189 kb on - strand at 1718.189 kb on - strand at 1718.202 kb on - strand at 1718.202 kb on - strand at 1718.214 kb on - strand at 1718.214 kb on - strand at 1718.220 kb on + strand at 1718.220 kb on + strand at 1718.255 kb on - strand at 1718.255 kb on - strand at 1718.270 kb on - strand at 1718.292 kb on + strand at 1718.292 kb on + strand at 1718.339 kb on - strand at 1718.339 kb on - strand at 1718.339 kb on - strand at 1718.479 kb on - strand, within PP_1514 at 1718.508 kb on + strand, within PP_1514 at 1718.508 kb on + strand, within PP_1514 at 1718.509 kb on - strand, within PP_1514 at 1718.509 kb on - strand, within PP_1514 at 1718.598 kb on + strand, within PP_1514 at 1718.598 kb on + strand, within PP_1514 at 1718.599 kb on - strand, within PP_1514 at 1718.599 kb on - strand, within PP_1514 at 1718.616 kb on + strand, within PP_1514 at 1718.617 kb on - strand, within PP_1514 at 1718.617 kb on - strand, within PP_1514 at 1718.697 kb on + strand, within PP_1514 at 1718.698 kb on - strand at 1718.698 kb on - strand, within PP_1514 at 1718.698 kb on - strand, within PP_1514 at 1718.698 kb on - strand, within PP_1514 at 1718.703 kb on + strand, within PP_1514 at 1718.703 kb on + strand, within PP_1514 at 1718.756 kb on + strand, within PP_1514 at 1718.809 kb on + strand, within PP_1514 at 1718.820 kb on - strand, within PP_1514 at 1718.820 kb on - strand, within PP_1514 at 1718.828 kb on - strand, within PP_1514 at 1718.870 kb on + strand, within PP_1514 at 1718.871 kb on - strand, within PP_1514 at 1718.876 kb on - strand, within PP_1514 at 1718.885 kb on + strand, within PP_1514 at 1718.885 kb on + strand, within PP_1514 at 1718.923 kb on + strand, within PP_1514 at 1718.924 kb on - strand, within PP_1514 at 1718.972 kb on + strand, within PP_1514 at 1719.161 kb on + strand at 1719.161 kb on + strand at 1719.161 kb on + strand at 1719.161 kb on + strand at 1719.162 kb on - strand at 1719.162 kb on - strand at 1719.190 kb on + strand at 1719.190 kb on + strand at 1719.262 kb on + strand at 1719.263 kb on - strand at 1719.271 kb on + strand at 1719.271 kb on + strand at 1719.272 kb on - strand at 1719.272 kb on - strand at 1719.292 kb on + strand at 1719.293 kb on - strand at 1719.293 kb on - strand at 1719.354 kb on + strand at 1719.355 kb on - strand at 1719.355 kb on - strand at 1719.387 kb on + strand at 1719.388 kb on - strand at 1719.388 kb on - strand at 1719.399 kb on + strand at 1719.399 kb on + strand at 1719.399 kb on + strand at 1719.399 kb on + strand at 1719.564 kb on + strand, within PP_1515 at 1719.607 kb on + strand, within PP_1515 at 1719.608 kb on - strand, within PP_1515 at 1719.614 kb on - strand, within PP_1515 at 1719.614 kb on - strand, within PP_1515 at 1719.690 kb on - strand, within PP_1515 at 1719.690 kb on - strand, within PP_1515 at 1719.690 kb on - strand, within PP_1515 at 1719.751 kb on + strand, within PP_1515 at 1719.838 kb on + strand, within PP_1515 at 1720.062 kb on - strand at 1720.073 kb on - strand at 1720.209 kb on - strand at 1720.214 kb on - strand at 1720.230 kb on - strand at 1720.232 kb on + strand at 1720.232 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 1,717,351 - +1.5 1,717,351 - +0.1 1,717,594 + PP_1513 0.38 -0.4 1,717,825 - PP_1513 0.67 -0.4 1,717,825 - PP_1513 0.67 -0.1 1,718,019 - -1.5 1,718,031 - -0.4 1,718,044 - +0.8 1,718,052 + -2.0 1,718,052 + +1.2 1,718,053 - -0.4 1,718,087 + -1.5 1,718,088 - -0.3 1,718,110 + -0.3 1,718,110 + +0.4 1,718,110 + +0.8 1,718,111 - +0.4 1,718,111 - -0.2 1,718,111 - +1.3 1,718,111 - -0.3 1,718,176 + -2.5 1,718,188 + -0.5 1,718,188 + -1.3 1,718,189 - +0.5 1,718,189 - +0.6 1,718,202 - -1.7 1,718,202 - +0.8 1,718,214 - +1.5 1,718,214 - +0.4 1,718,220 + -0.4 1,718,220 + -1.1 1,718,255 - +2.1 1,718,255 - -0.7 1,718,270 - -0.9 1,718,292 + -1.8 1,718,292 + -0.9 1,718,339 - +1.8 1,718,339 - +0.4 1,718,339 - -1.6 1,718,479 - PP_1514 0.21 +0.8 1,718,508 + PP_1514 0.24 -4.1 1,718,508 + PP_1514 0.24 +0.5 1,718,509 - PP_1514 0.25 -0.3 1,718,509 - PP_1514 0.25 +0.8 1,718,598 + PP_1514 0.34 -0.2 1,718,598 + PP_1514 0.34 +3.3 1,718,599 - PP_1514 0.34 +1.1 1,718,599 - PP_1514 0.34 +0.8 1,718,616 + PP_1514 0.36 -3.2 1,718,617 - PP_1514 0.36 +0.5 1,718,617 - PP_1514 0.36 -0.5 1,718,697 + PP_1514 0.44 +1.6 1,718,698 - +0.8 1,718,698 - PP_1514 0.44 +0.0 1,718,698 - PP_1514 0.44 +0.4 1,718,698 - PP_1514 0.44 +2.1 1,718,703 + PP_1514 0.45 +1.2 1,718,703 + PP_1514 0.45 +0.1 1,718,756 + PP_1514 0.50 -1.9 1,718,809 + PP_1514 0.56 -0.4 1,718,820 - PP_1514 0.57 +1.6 1,718,820 - PP_1514 0.57 +0.9 1,718,828 - PP_1514 0.57 +0.8 1,718,870 + PP_1514 0.62 +0.0 1,718,871 - PP_1514 0.62 -1.8 1,718,876 - PP_1514 0.62 +1.7 1,718,885 + PP_1514 0.63 -1.0 1,718,885 + PP_1514 0.63 -0.4 1,718,923 + PP_1514 0.67 +0.6 1,718,924 - PP_1514 0.67 -3.3 1,718,972 + PP_1514 0.72 +2.9 1,719,161 + -1.2 1,719,161 + +0.7 1,719,161 + +3.8 1,719,161 + -0.5 1,719,162 - -1.3 1,719,162 - +0.8 1,719,190 + +0.4 1,719,190 + +0.5 1,719,262 + -1.3 1,719,263 - -0.7 1,719,271 + +3.8 1,719,271 + +1.9 1,719,272 - +1.6 1,719,272 - -0.6 1,719,292 + -0.9 1,719,293 - +2.8 1,719,293 - +0.2 1,719,354 + -0.4 1,719,355 - -1.3 1,719,355 - +3.1 1,719,387 + -0.2 1,719,388 - +0.4 1,719,388 - +1.6 1,719,399 + -0.0 1,719,399 + -0.1 1,719,399 + +1.1 1,719,399 + +0.4 1,719,564 + PP_1515 0.30 +0.8 1,719,607 + PP_1515 0.37 -0.0 1,719,608 - PP_1515 0.37 -1.0 1,719,614 - PP_1515 0.38 +1.0 1,719,614 - PP_1515 0.38 -1.5 1,719,690 - PP_1515 0.50 -0.2 1,719,690 - PP_1515 0.50 +2.5 1,719,690 - PP_1515 0.50 -0.1 1,719,751 + PP_1515 0.60 +0.1 1,719,838 + PP_1515 0.73 +1.1 1,720,062 - +0.4 1,720,073 - +1.1 1,720,209 - -0.7 1,720,214 - +0.9 1,720,230 - +0.8 1,720,232 + +1.3 1,720,232 + +0.5
Or see this region's nucleotide sequence