Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0704 and PP_0705 are separated by 171 nucleotides PP_0705 and PP_0706 are separated by 47 nucleotides PP_0706 and PP_0707 are separated by 56 nucleotides
PP_0704: PP_0704 - RNA polymerase sigma-70 factor, ECF subfamily, at 818,160 to 818,675
_0704
PP_0705: PP_0705 - DNA-3-methyladenine glycosylase, at 818,847 to 819,473
_0705
PP_0706: PP_0706 - fused O(6)-alkylguanine DNA alkyltransferase - transcriptional activator, at 819,521 to 820,573
_0706
PP_0707: PP_0707 - putative membrane protein, at 820,630 to 823,041
_0707
Position (kb) 819
820
821 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 818.546 kb on + strand, within PP_0704 at 818.615 kb on + strand, within PP_0704 at 818.616 kb on - strand, within PP_0704 at 818.616 kb on - strand, within PP_0704 at 818.630 kb on - strand at 818.678 kb on + strand at 818.679 kb on - strand at 818.742 kb on + strand at 818.743 kb on - strand at 818.743 kb on - strand at 818.770 kb on + strand at 818.770 kb on + strand at 818.771 kb on - strand at 818.812 kb on + strand at 818.878 kb on - strand at 818.930 kb on + strand, within PP_0705 at 818.964 kb on - strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.983 kb on - strand, within PP_0705 at 818.983 kb on - strand, within PP_0705 at 818.983 kb on - strand, within PP_0705 at 819.303 kb on + strand, within PP_0705 at 819.304 kb on - strand, within PP_0705 at 819.304 kb on - strand, within PP_0705 at 819.304 kb on - strand, within PP_0705 at 819.456 kb on + strand at 819.457 kb on - strand at 819.457 kb on - strand at 819.491 kb on + strand at 819.491 kb on + strand at 819.506 kb on - strand at 819.530 kb on + strand at 819.530 kb on + strand at 819.606 kb on + strand at 819.606 kb on + strand at 819.606 kb on + strand at 819.607 kb on - strand at 819.610 kb on + strand at 819.610 kb on + strand at 819.610 kb on + strand at 819.611 kb on - strand at 819.611 kb on - strand at 819.661 kb on + strand, within PP_0706 at 819.662 kb on - strand, within PP_0706 at 819.677 kb on + strand, within PP_0706 at 819.730 kb on + strand, within PP_0706 at 819.731 kb on - strand, within PP_0706 at 819.973 kb on + strand, within PP_0706 at 819.973 kb on + strand, within PP_0706 at 819.973 kb on + strand, within PP_0706 at 819.974 kb on - strand, within PP_0706 at 819.974 kb on - strand, within PP_0706 at 819.990 kb on - strand, within PP_0706 at 820.010 kb on + strand, within PP_0706 at 820.010 kb on + strand, within PP_0706 at 820.011 kb on - strand, within PP_0706 at 820.011 kb on - strand, within PP_0706 at 820.116 kb on + strand, within PP_0706 at 820.117 kb on - strand, within PP_0706 at 820.117 kb on - strand, within PP_0706 at 820.239 kb on - strand, within PP_0706 at 820.382 kb on + strand, within PP_0706 at 820.383 kb on - strand, within PP_0706 at 820.383 kb on - strand, within PP_0706 at 820.456 kb on + strand, within PP_0706 at 820.457 kb on - strand, within PP_0706 at 820.457 kb on - strand, within PP_0706 at 820.625 kb on - strand at 821.037 kb on - strand, within PP_0707 at 821.204 kb on + strand, within PP_0707 at 821.324 kb on + strand, within PP_0707 at 821.325 kb on - strand, within PP_0707 at 821.527 kb on - strand, within PP_0707
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 818,546 + PP_0704 0.75 -0.6 818,615 + PP_0704 0.88 +0.8 818,616 - PP_0704 0.88 +0.3 818,616 - PP_0704 0.88 -0.2 818,630 - -0.4 818,678 + -1.0 818,679 - +0.1 818,742 + -1.0 818,743 - +1.5 818,743 - -1.1 818,770 + -1.5 818,770 + +0.1 818,771 - -3.5 818,812 + -0.6 818,878 - -0.6 818,930 + PP_0705 0.13 +0.8 818,964 - PP_0705 0.19 -1.9 818,982 + PP_0705 0.22 +2.8 818,982 + PP_0705 0.22 +1.7 818,982 + PP_0705 0.22 +1.6 818,983 - PP_0705 0.22 -2.6 818,983 - PP_0705 0.22 +1.7 818,983 - PP_0705 0.22 -0.7 819,303 + PP_0705 0.73 -2.2 819,304 - PP_0705 0.73 +4.0 819,304 - PP_0705 0.73 +0.3 819,304 - PP_0705 0.73 -0.8 819,456 + +1.8 819,457 - -1.3 819,457 - -3.0 819,491 + -0.3 819,491 + -1.8 819,506 - -2.2 819,530 + +0.6 819,530 + -1.5 819,606 + -0.4 819,606 + +0.2 819,606 + -0.4 819,607 - -1.9 819,610 + +0.8 819,610 + -1.0 819,610 + -0.5 819,611 - -3.2 819,611 - +0.3 819,661 + PP_0706 0.13 +1.6 819,662 - PP_0706 0.13 +0.6 819,677 + PP_0706 0.15 +1.8 819,730 + PP_0706 0.20 +0.8 819,731 - PP_0706 0.20 +0.8 819,973 + PP_0706 0.43 +0.9 819,973 + PP_0706 0.43 +2.5 819,973 + PP_0706 0.43 +1.1 819,974 - PP_0706 0.43 +0.6 819,974 - PP_0706 0.43 +0.4 819,990 - PP_0706 0.45 -0.7 820,010 + PP_0706 0.46 -2.0 820,010 + PP_0706 0.46 -2.0 820,011 - PP_0706 0.47 +3.6 820,011 - PP_0706 0.47 +0.8 820,116 + PP_0706 0.57 -0.9 820,117 - PP_0706 0.57 -2.0 820,117 - PP_0706 0.57 +5.8 820,239 - PP_0706 0.68 -3.2 820,382 + PP_0706 0.82 +0.1 820,383 - PP_0706 0.82 -1.6 820,383 - PP_0706 0.82 +2.6 820,456 + PP_0706 0.89 -0.7 820,457 - PP_0706 0.89 +1.3 820,457 - PP_0706 0.89 +2.4 820,625 - -2.4 821,037 - PP_0707 0.17 -1.1 821,204 + PP_0707 0.24 +3.3 821,324 + PP_0707 0.29 -1.5 821,325 - PP_0707 0.29 -2.1 821,527 - PP_0707 0.37 -1.5
Or see this region's nucleotide sequence