Experiment: pH 8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0660 and PP_0661 are separated by 45 nucleotides PP_0661 and PP_0662 are separated by 24 nucleotides PP_0662 and PP_0663 overlap by 4 nucleotides PP_0663 and PP_0664 are separated by 198 nucleotides
PP_0660: PP_0660 - S-methyl-L-methionine transporter, at 767,923 to 769,362
_0660
PP_0661: PP_0661 - putative Transcriptional regulator AmpR, at 769,408 to 770,292
_0661
PP_0662: PP_0662 - putative Threonine synthase, at 770,317 to 771,705
_0662
PP_0663: PP_0663 - Transcriptional regulator, AsnC family, at 771,702 to 772,139
_0663
PP_0664: PP_0664 - homoserine dehydrogenase, at 772,338 to 773,381
_0664
Position (kb) 770
771
772 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 769.372 kb on - strand at 769.372 kb on - strand at 769.372 kb on - strand at 769.374 kb on + strand at 769.374 kb on + strand at 769.374 kb on + strand at 769.374 kb on + strand at 769.374 kb on + strand at 769.506 kb on + strand, within PP_0661 at 769.506 kb on + strand, within PP_0661 at 769.615 kb on + strand, within PP_0661 at 769.615 kb on + strand, within PP_0661 at 769.615 kb on + strand, within PP_0661 at 769.616 kb on - strand, within PP_0661 at 769.638 kb on + strand, within PP_0661 at 769.703 kb on + strand, within PP_0661 at 769.789 kb on + strand, within PP_0661 at 769.790 kb on - strand, within PP_0661 at 769.790 kb on - strand, within PP_0661 at 770.012 kb on - strand, within PP_0661 at 770.012 kb on - strand, within PP_0661 at 770.138 kb on + strand, within PP_0661 at 770.290 kb on + strand at 770.291 kb on - strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.296 kb on - strand at 770.296 kb on - strand at 770.296 kb on - strand at 770.299 kb on + strand at 770.299 kb on + strand at 770.299 kb on + strand at 770.299 kb on + strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.303 kb on + strand at 770.304 kb on - strand at 770.304 kb on - strand at 770.304 kb on - strand at 770.323 kb on + strand at 770.324 kb on - strand at 770.324 kb on - strand at 770.324 kb on - strand at 770.324 kb on - strand at 770.404 kb on + strand at 770.404 kb on + strand at 770.526 kb on + strand, within PP_0662 at 770.555 kb on - strand, within PP_0662 at 770.555 kb on - strand, within PP_0662 at 770.805 kb on - strand, within PP_0662 at 770.805 kb on - strand, within PP_0662 at 770.806 kb on + strand, within PP_0662 at 770.807 kb on - strand, within PP_0662 at 770.807 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.094 kb on + strand, within PP_0662 at 771.094 kb on + strand, within PP_0662 at 771.095 kb on - strand, within PP_0662 at 771.095 kb on - strand, within PP_0662 at 771.095 kb on - strand, within PP_0662 at 771.196 kb on + strand, within PP_0662 at 771.283 kb on + strand, within PP_0662 at 771.283 kb on + strand, within PP_0662 at 771.284 kb on - strand, within PP_0662 at 771.284 kb on - strand, within PP_0662 at 771.284 kb on - strand, within PP_0662 at 771.394 kb on + strand, within PP_0662 at 771.395 kb on - strand, within PP_0662 at 771.461 kb on + strand, within PP_0662 at 771.485 kb on + strand, within PP_0662 at 771.486 kb on - strand, within PP_0662 at 771.863 kb on + strand, within PP_0663 at 771.863 kb on + strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.924 kb on + strand, within PP_0663 at 771.924 kb on + strand, within PP_0663 at 771.931 kb on - strand, within PP_0663 at 772.159 kb on + strand at 772.249 kb on + strand at 772.250 kb on - strand at 772.254 kb on - strand at 772.254 kb on - strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.294 kb on - strand at 772.298 kb on + strand at 772.299 kb on - strand at 772.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction pH 8 remove 769,372 - -1.6 769,372 - +0.5 769,372 - +0.5 769,374 + -2.0 769,374 + -0.3 769,374 + -1.6 769,374 + +0.8 769,374 + +2.2 769,506 + PP_0661 0.11 +0.7 769,506 + PP_0661 0.11 -3.2 769,615 + PP_0661 0.23 -1.5 769,615 + PP_0661 0.23 -0.0 769,615 + PP_0661 0.23 -1.8 769,616 - PP_0661 0.24 +3.0 769,638 + PP_0661 0.26 -0.2 769,703 + PP_0661 0.33 +1.3 769,789 + PP_0661 0.43 -0.4 769,790 - PP_0661 0.43 -0.4 769,790 - PP_0661 0.43 +1.4 770,012 - PP_0661 0.68 -0.1 770,012 - PP_0661 0.68 -0.2 770,138 + PP_0661 0.82 -1.7 770,290 + +0.2 770,291 - -1.1 770,295 + -0.3 770,295 + -2.3 770,295 + -1.2 770,295 + -1.2 770,295 + +0.8 770,295 + +0.3 770,295 + +0.6 770,295 + +1.0 770,296 - -0.4 770,296 - -2.0 770,296 - +2.1 770,299 + -0.1 770,299 + -2.2 770,299 + +0.1 770,299 + +0.5 770,300 - +0.6 770,300 - +0.5 770,300 - -2.0 770,300 - +0.0 770,300 - +0.1 770,300 - +0.6 770,300 - -2.5 770,300 - +1.3 770,303 + -0.2 770,304 - +1.4 770,304 - +0.8 770,304 - -0.2 770,323 + -0.5 770,324 - -0.9 770,324 - +2.3 770,324 - +0.8 770,324 - -1.0 770,404 + -1.0 770,404 + +2.7 770,526 + PP_0662 0.15 -2.1 770,555 - PP_0662 0.17 -0.4 770,555 - PP_0662 0.17 -0.0 770,805 - PP_0662 0.35 +3.2 770,805 - PP_0662 0.35 -0.9 770,806 + PP_0662 0.35 -0.4 770,807 - PP_0662 0.35 -2.9 770,807 - PP_0662 0.35 +0.8 771,014 - PP_0662 0.50 +1.2 771,014 - PP_0662 0.50 -0.7 771,014 - PP_0662 0.50 -0.5 771,014 - PP_0662 0.50 -0.4 771,014 - PP_0662 0.50 +1.8 771,094 + PP_0662 0.56 -1.1 771,094 + PP_0662 0.56 -2.2 771,095 - PP_0662 0.56 +0.7 771,095 - PP_0662 0.56 -3.5 771,095 - PP_0662 0.56 -2.0 771,196 + PP_0662 0.63 +0.3 771,283 + PP_0662 0.70 -1.0 771,283 + PP_0662 0.70 +0.5 771,284 - PP_0662 0.70 -1.0 771,284 - PP_0662 0.70 -0.6 771,284 - PP_0662 0.70 -0.8 771,394 + PP_0662 0.78 -2.8 771,395 - PP_0662 0.78 -2.5 771,461 + PP_0662 0.82 -0.9 771,485 + PP_0662 0.84 +0.5 771,486 - PP_0662 0.84 -1.1 771,863 + PP_0663 0.37 +0.0 771,863 + PP_0663 0.37 +3.8 771,864 - PP_0663 0.37 +0.4 771,864 - PP_0663 0.37 -1.0 771,864 - PP_0663 0.37 +2.2 771,924 + PP_0663 0.51 -0.8 771,924 + PP_0663 0.51 +1.8 771,931 - PP_0663 0.52 +1.8 772,159 + +3.1 772,249 + +4.2 772,250 - +0.8 772,254 - +1.3 772,254 - +1.1 772,293 + -0.7 772,293 + -1.9 772,293 + +0.5 772,293 + -3.0 772,293 + -2.4 772,293 + +2.8 772,294 - -1.3 772,298 + -1.3 772,299 - -2.5 772,299 - -0.2
Or see this region's nucleotide sequence