Experiment: pH 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5155 and PP_5156 are separated by 47 nucleotides PP_5156 and PP_5157 are separated by 223 nucleotides PP_5157 and PP_5158 are separated by 119 nucleotides PP_5158 and PP_5159 overlap by 14 nucleotides
PP_5155: PP_5155 - D-3-phosphoglycerate dehydrogenase / alpha-ketoglutarate reductase, at 5,881,440 to 5,882,669
_5155
PP_5156: PP_5156 - conserved exported protein of unknown function, at 5,882,717 to 5,883,193
_5156
PP_5157: PP_5157 - conserved exported protein of unknown function, at 5,883,417 to 5,883,905
_5157
PP_5158: PP_5158 - conserved protein of unknown function, at 5,884,025 to 5,884,528
_5158
PP_5159: PP_5159 - conserved protein of unknown function, at 5,884,515 to 5,885,138
_5159
Position (kb)
5883
5884 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5882.575 kb on - strand at 5882.582 kb on - strand at 5882.582 kb on - strand at 5882.602 kb on - strand at 5882.602 kb on - strand at 5882.667 kb on + strand at 5882.668 kb on - strand at 5882.668 kb on - strand at 5882.668 kb on - strand at 5882.668 kb on - strand at 5882.668 kb on - strand at 5882.668 kb on - strand at 5882.668 kb on - strand at 5882.756 kb on - strand at 5882.775 kb on + strand, within PP_5156 at 5882.775 kb on + strand, within PP_5156 at 5882.775 kb on + strand, within PP_5156 at 5882.776 kb on - strand, within PP_5156 at 5882.824 kb on + strand at 5883.010 kb on + strand, within PP_5156 at 5883.183 kb on - strand at 5883.215 kb on + strand at 5883.215 kb on + strand at 5883.215 kb on + strand at 5883.258 kb on + strand at 5883.259 kb on - strand at 5883.259 kb on - strand at 5883.268 kb on - strand at 5883.268 kb on - strand at 5883.268 kb on - strand at 5883.302 kb on - strand at 5883.342 kb on + strand at 5883.343 kb on - strand at 5883.414 kb on + strand at 5883.414 kb on + strand at 5883.414 kb on + strand at 5883.415 kb on - strand at 5883.415 kb on - strand at 5883.415 kb on - strand at 5883.415 kb on - strand at 5883.415 kb on - strand at 5883.415 kb on - strand at 5883.431 kb on - strand at 5883.431 kb on - strand at 5883.436 kb on + strand at 5883.437 kb on - strand at 5883.437 kb on - strand at 5883.448 kb on + strand at 5883.448 kb on + strand at 5883.449 kb on - strand at 5883.521 kb on - strand, within PP_5157 at 5883.540 kb on + strand, within PP_5157 at 5883.678 kb on + strand, within PP_5157 at 5883.678 kb on + strand, within PP_5157 at 5883.679 kb on - strand, within PP_5157 at 5883.736 kb on + strand, within PP_5157 at 5883.736 kb on + strand, within PP_5157 at 5883.737 kb on - strand, within PP_5157 at 5883.737 kb on - strand, within PP_5157 at 5883.737 kb on - strand, within PP_5157 at 5883.737 kb on - strand, within PP_5157 at 5883.737 kb on - strand, within PP_5157 at 5883.903 kb on + strand at 5883.904 kb on - strand at 5883.955 kb on - strand at 5883.965 kb on - strand at 5884.034 kb on + strand at 5884.034 kb on + strand at 5884.034 kb on + strand at 5884.034 kb on + strand at 5884.034 kb on + strand at 5884.035 kb on - strand at 5884.035 kb on - strand at 5884.035 kb on - strand at 5884.035 kb on - strand at 5884.035 kb on - strand at 5884.036 kb on + strand at 5884.036 kb on + strand at 5884.036 kb on + strand at 5884.037 kb on - strand at 5884.037 kb on - strand at 5884.037 kb on - strand at 5884.037 kb on - strand at 5884.040 kb on - strand at 5884.161 kb on + strand, within PP_5158 at 5884.161 kb on + strand, within PP_5158 at 5884.161 kb on + strand, within PP_5158 at 5884.161 kb on + strand, within PP_5158 at 5884.161 kb on + strand, within PP_5158 at 5884.161 kb on + strand, within PP_5158 at 5884.161 kb on + strand, within PP_5158 at 5884.162 kb on - strand, within PP_5158 at 5884.162 kb on - strand, within PP_5158 at 5884.162 kb on - strand, within PP_5158 at 5884.162 kb on - strand, within PP_5158 at 5884.208 kb on + strand, within PP_5158 at 5884.382 kb on + strand, within PP_5158 at 5884.382 kb on + strand, within PP_5158 at 5884.383 kb on - strand, within PP_5158 at 5884.383 kb on - strand, within PP_5158 at 5884.465 kb on - strand, within PP_5158 at 5884.585 kb on - strand, within PP_5159 at 5884.614 kb on + strand, within PP_5159 at 5884.614 kb on + strand, within PP_5159 at 5884.615 kb on - strand, within PP_5159 at 5884.717 kb on + strand, within PP_5159 at 5884.717 kb on + strand, within PP_5159 at 5884.778 kb on - strand, within PP_5159 at 5884.795 kb on + strand, within PP_5159 at 5884.796 kb on - strand, within PP_5159
Per-strain Table
Position Strand Gene LocusTag Fraction pH 6 remove 5,882,575 - -2.2 5,882,582 - -2.0 5,882,582 - -3.6 5,882,602 - +0.8 5,882,602 - +0.8 5,882,667 + -1.9 5,882,668 - -2.8 5,882,668 - -1.5 5,882,668 - -1.3 5,882,668 - +0.8 5,882,668 - -1.0 5,882,668 - +0.2 5,882,668 - -1.2 5,882,756 - +0.2 5,882,775 + PP_5156 0.12 +0.0 5,882,775 + PP_5156 0.12 +0.8 5,882,775 + PP_5156 0.12 +0.9 5,882,776 - PP_5156 0.12 -0.4 5,882,824 + +0.8 5,883,010 + PP_5156 0.61 +0.8 5,883,183 - +2.1 5,883,215 + -0.1 5,883,215 + +2.8 5,883,215 + +2.6 5,883,258 + -0.8 5,883,259 - +1.1 5,883,259 - +3.4 5,883,268 - +0.9 5,883,268 - -2.4 5,883,268 - -0.7 5,883,302 - -0.8 5,883,342 + +0.5 5,883,343 - +3.1 5,883,414 + -0.2 5,883,414 + +1.6 5,883,414 + +1.1 5,883,415 - -2.7 5,883,415 - +0.6 5,883,415 - -2.0 5,883,415 - -0.1 5,883,415 - -0.2 5,883,415 - +1.1 5,883,431 - -0.2 5,883,431 - -1.2 5,883,436 + +0.3 5,883,437 - +3.4 5,883,437 - -1.7 5,883,448 + +0.1 5,883,448 + +2.8 5,883,449 - -2.6 5,883,521 - PP_5157 0.21 -0.2 5,883,540 + PP_5157 0.25 +0.8 5,883,678 + PP_5157 0.53 -0.2 5,883,678 + PP_5157 0.53 -1.5 5,883,679 - PP_5157 0.54 +0.0 5,883,736 + PP_5157 0.65 +2.9 5,883,736 + PP_5157 0.65 +1.4 5,883,737 - PP_5157 0.65 +3.8 5,883,737 - PP_5157 0.65 +2.4 5,883,737 - PP_5157 0.65 +0.8 5,883,737 - PP_5157 0.65 +0.7 5,883,737 - PP_5157 0.65 +2.0 5,883,903 + -0.1 5,883,904 - -0.4 5,883,955 - -0.6 5,883,965 - +0.1 5,884,034 + +1.6 5,884,034 + +0.9 5,884,034 + +0.1 5,884,034 + +1.2 5,884,034 + -1.8 5,884,035 - +0.9 5,884,035 - -1.0 5,884,035 - -0.3 5,884,035 - +0.1 5,884,035 - +0.8 5,884,036 + +0.4 5,884,036 + -1.8 5,884,036 + -1.4 5,884,037 - -1.6 5,884,037 - -1.1 5,884,037 - +0.2 5,884,037 - -0.5 5,884,040 - -0.5 5,884,161 + PP_5158 0.27 +0.0 5,884,161 + PP_5158 0.27 -1.1 5,884,161 + PP_5158 0.27 +0.8 5,884,161 + PP_5158 0.27 -1.8 5,884,161 + PP_5158 0.27 +1.6 5,884,161 + PP_5158 0.27 -2.4 5,884,161 + PP_5158 0.27 -0.4 5,884,162 - PP_5158 0.27 -1.0 5,884,162 - PP_5158 0.27 +0.5 5,884,162 - PP_5158 0.27 -0.9 5,884,162 - PP_5158 0.27 -0.8 5,884,208 + PP_5158 0.36 -0.3 5,884,382 + PP_5158 0.71 -0.6 5,884,382 + PP_5158 0.71 +1.3 5,884,383 - PP_5158 0.71 -1.6 5,884,383 - PP_5158 0.71 -0.9 5,884,465 - PP_5158 0.87 -1.7 5,884,585 - PP_5159 0.11 -0.8 5,884,614 + PP_5159 0.16 +3.6 5,884,614 + PP_5159 0.16 -1.4 5,884,615 - PP_5159 0.16 -1.9 5,884,717 + PP_5159 0.32 -2.3 5,884,717 + PP_5159 0.32 +0.2 5,884,778 - PP_5159 0.42 -3.7 5,884,795 + PP_5159 0.45 +2.4 5,884,796 - PP_5159 0.45 -1.6
Or see this region's nucleotide sequence