Experiment: pH 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0300 and PP_0301 are separated by 99 nucleotides PP_0301 and PP_0302 are separated by 10 nucleotides PP_0302 and PP_0303 are separated by 166 nucleotides
PP_0300: PP_0300 - putative metallopeptidase, at 360,350 to 361,936
_0300
PP_0301: PP_0301 - betainyl-CoA thiolase, at 362,036 to 362,506
_0301
PP_0302: PP_0302 - L-carnitine 3-dehydrogenase (EC 1.1.1.108) <small>(from data)</small>, at 362,517 to 363,482
_0302
PP_0303: PP_0303 - dehydrocarnitine cleavage enzyme, at 363,649 to 364,533
_0303
Position (kb) 362
363
364 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 361.538 kb on - strand, within PP_0300 at 361.628 kb on + strand, within PP_0300 at 361.738 kb on - strand, within PP_0300 at 361.805 kb on - strand at 361.854 kb on + strand at 361.855 kb on - strand at 361.917 kb on - strand at 361.928 kb on + strand at 361.957 kb on + strand at 361.958 kb on - strand at 362.000 kb on + strand at 362.005 kb on - strand at 362.005 kb on - strand at 362.005 kb on - strand at 362.005 kb on - strand at 362.056 kb on + strand at 362.066 kb on - strand at 362.067 kb on + strand at 362.068 kb on - strand at 362.068 kb on - strand at 362.100 kb on - strand, within PP_0301 at 362.174 kb on + strand, within PP_0301 at 362.175 kb on - strand, within PP_0301 at 362.232 kb on + strand, within PP_0301 at 362.233 kb on - strand, within PP_0301 at 362.233 kb on - strand, within PP_0301 at 362.313 kb on + strand, within PP_0301 at 362.314 kb on - strand, within PP_0301 at 362.321 kb on - strand, within PP_0301 at 362.409 kb on + strand, within PP_0301 at 362.409 kb on + strand, within PP_0301 at 362.427 kb on + strand, within PP_0301 at 362.454 kb on + strand, within PP_0301 at 362.519 kb on - strand at 362.519 kb on - strand at 362.695 kb on + strand, within PP_0302 at 362.695 kb on + strand, within PP_0302 at 362.696 kb on - strand, within PP_0302 at 362.696 kb on - strand, within PP_0302 at 362.771 kb on + strand, within PP_0302 at 362.771 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.773 kb on + strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.774 kb on - strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.777 kb on + strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.778 kb on - strand, within PP_0302 at 362.969 kb on - strand, within PP_0302 at 362.969 kb on - strand, within PP_0302 at 362.991 kb on - strand, within PP_0302 at 362.991 kb on - strand, within PP_0302 at 363.044 kb on - strand, within PP_0302 at 363.044 kb on - strand, within PP_0302 at 363.100 kb on + strand, within PP_0302 at 363.100 kb on + strand, within PP_0302 at 363.101 kb on - strand, within PP_0302 at 363.101 kb on - strand, within PP_0302 at 363.482 kb on + strand at 363.482 kb on + strand at 363.482 kb on + strand at 363.483 kb on - strand at 363.483 kb on - strand at 363.483 kb on - strand at 363.499 kb on - strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.508 kb on + strand at 363.509 kb on - strand at 363.509 kb on - strand at 363.509 kb on - strand at 363.780 kb on - strand, within PP_0303 at 363.780 kb on - strand, within PP_0303 at 363.780 kb on - strand, within PP_0303 at 364.109 kb on + strand, within PP_0303 at 364.109 kb on + strand, within PP_0303 at 364.110 kb on - strand, within PP_0303 at 364.110 kb on - strand, within PP_0303 at 364.426 kb on + strand, within PP_0303
Per-strain Table
Position Strand Gene LocusTag Fraction pH 6 remove 361,538 - PP_0300 0.75 +0.2 361,628 + PP_0300 0.81 -0.6 361,738 - PP_0300 0.87 -0.4 361,805 - -0.0 361,854 + +2.8 361,855 - -0.3 361,917 - +1.0 361,928 + -0.7 361,957 + +0.9 361,958 - -2.8 362,000 + +2.5 362,005 - -1.9 362,005 - -1.5 362,005 - +0.9 362,005 - -0.3 362,056 + -0.2 362,066 - +1.9 362,067 + +1.1 362,068 - +0.9 362,068 - -1.3 362,100 - PP_0301 0.14 +3.5 362,174 + PP_0301 0.29 -0.3 362,175 - PP_0301 0.30 +1.9 362,232 + PP_0301 0.42 -0.5 362,233 - PP_0301 0.42 +1.1 362,233 - PP_0301 0.42 +0.9 362,313 + PP_0301 0.59 -0.1 362,314 - PP_0301 0.59 +0.7 362,321 - PP_0301 0.61 +0.6 362,409 + PP_0301 0.79 -2.7 362,409 + PP_0301 0.79 +3.5 362,427 + PP_0301 0.83 -1.1 362,454 + PP_0301 0.89 -0.0 362,519 - -0.5 362,519 - +1.0 362,695 + PP_0302 0.18 +0.6 362,695 + PP_0302 0.18 -0.1 362,696 - PP_0302 0.19 -0.3 362,696 - PP_0302 0.19 +3.6 362,771 + PP_0302 0.26 +3.9 362,771 + PP_0302 0.26 +0.3 362,773 + PP_0302 0.27 -1.7 362,773 + PP_0302 0.27 -0.9 362,773 + PP_0302 0.27 +3.9 362,774 - PP_0302 0.27 -1.2 362,774 - PP_0302 0.27 +4.2 362,774 - PP_0302 0.27 -1.1 362,774 - PP_0302 0.27 +1.5 362,774 - PP_0302 0.27 +0.5 362,774 - PP_0302 0.27 -0.8 362,777 + PP_0302 0.27 -0.5 362,777 + PP_0302 0.27 -0.7 362,777 + PP_0302 0.27 -3.5 362,778 - PP_0302 0.27 +2.0 362,778 - PP_0302 0.27 -1.0 362,778 - PP_0302 0.27 +0.4 362,778 - PP_0302 0.27 -0.7 362,778 - PP_0302 0.27 -0.5 362,969 - PP_0302 0.47 -2.5 362,969 - PP_0302 0.47 -0.7 362,991 - PP_0302 0.49 -0.9 362,991 - PP_0302 0.49 +0.2 363,044 - PP_0302 0.55 +1.4 363,044 - PP_0302 0.55 -1.7 363,100 + PP_0302 0.60 +0.7 363,100 + PP_0302 0.60 +0.9 363,101 - PP_0302 0.60 -0.2 363,101 - PP_0302 0.60 +2.6 363,482 + -0.3 363,482 + -1.5 363,482 + +0.9 363,483 - +1.3 363,483 - -0.6 363,483 - +0.9 363,499 - -0.1 363,508 + -1.8 363,508 + +0.9 363,508 + -3.8 363,508 + -2.8 363,508 + +1.2 363,509 - +1.1 363,509 - -1.3 363,509 - -0.9 363,780 - PP_0303 0.15 -0.1 363,780 - PP_0303 0.15 -1.7 363,780 - PP_0303 0.15 +1.9 364,109 + PP_0303 0.52 -2.5 364,109 + PP_0303 0.52 -1.7 364,110 - PP_0303 0.52 -1.3 364,110 - PP_0303 0.52 +0.9 364,426 + PP_0303 0.88 +0.9
Or see this region's nucleotide sequence