Experiment: pH 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_t55 and PP_t54 are separated by 74 nucleotides PP_t54 and PP_t53 are separated by 71 nucleotides PP_t53 and PP_t52 are separated by 106 nucleotides PP_t52 and PP_4472 are separated by 72 nucleotides PP_4472 and PP_4473 are separated by 167 nucleotides PP_4473 and PP_4474 are separated by 102 nucleotides
PP_t55: PP_t55 - tRNA-Arg, at 5,079,302 to 5,079,378
_t55
PP_t54: PP_t54 - tRNA-Arg, at 5,079,453 to 5,079,529
_t54
PP_t53: PP_t53 - tRNA-Arg, at 5,079,601 to 5,079,677
_t53
PP_t52: PP_t52 - tRNA-Ser, at 5,079,784 to 5,079,874
_t52
PP_4472: PP_4472 - carbon storage regulator, at 5,079,947 to 5,080,135
_4472
PP_4473: PP_4473 - aspartokinase, at 5,080,303 to 5,081,538
_4473
PP_4474: PP_4474 - Alanine--tRNA ligase, at 5,081,641 to 5,084,265
_4474
Position (kb)
5080
5081
5082 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5079.370 kb on + strand, within PP_t55 at 5079.554 kb on - strand at 5079.695 kb on - strand at 5079.697 kb on - strand at 5080.039 kb on + strand, within PP_4472 at 5080.040 kb on - strand, within PP_4472 at 5080.135 kb on + strand at 5080.135 kb on + strand at 5080.135 kb on + strand at 5080.135 kb on + strand at 5080.135 kb on + strand at 5080.136 kb on - strand at 5080.136 kb on - strand at 5080.147 kb on + strand at 5080.147 kb on + strand at 5080.166 kb on + strand at 5080.304 kb on + strand at 5081.621 kb on - strand at 5081.621 kb on - strand at 5081.621 kb on - strand at 5081.621 kb on - strand at 5081.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction pH 5 remove 5,079,370 + PP_t55 0.88 +0.2 5,079,554 - +0.2 5,079,695 - +0.2 5,079,697 - +0.2 5,080,039 + PP_4472 0.49 +0.2 5,080,040 - PP_4472 0.49 -0.5 5,080,135 + -4.4 5,080,135 + -2.2 5,080,135 + +0.2 5,080,135 + +3.0 5,080,135 + +1.7 5,080,136 - -4.0 5,080,136 - -3.7 5,080,147 + +0.2 5,080,147 + -1.6 5,080,166 + -0.9 5,080,304 + -0.2 5,081,621 - -1.0 5,081,621 - +0.9 5,081,621 - -0.1 5,081,621 - -1.5 5,081,621 - -0.1
Or see this region's nucleotide sequence