Experiment: pH 5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1470 and PP_1471 are separated by 59 nucleotides PP_1471 and PP_5471 are separated by 130 nucleotides PP_5471 and PP_1473 are separated by 79 nucleotides
PP_1470: PP_1470 - Homoserine dehydrogenase, at 1,674,834 to 1,676,138
_1470
PP_1471: PP_1471 - Threonine synthase, at 1,676,198 to 1,677,607
_1471
PP_5471: PP_5471 - putative response regulator receiver protein, at 1,677,738 to 1,678,157
_5471
PP_1473: PP_1473 - conserved protein of unknown function, at 1,678,237 to 1,678,518
_1473
Position (kb)
1676
1677
1678 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1675.716 kb on + strand at 1675.716 kb on + strand, within PP_1470 at 1675.733 kb on + strand at 1675.742 kb on + strand at 1675.887 kb on + strand, within PP_1470 at 1675.890 kb on + strand at 1676.143 kb on + strand at 1677.606 kb on - strand at 1677.666 kb on + strand at 1677.666 kb on + strand at 1677.681 kb on - strand at 1677.681 kb on - strand at 1677.681 kb on - strand at 1677.706 kb on - strand at 1677.706 kb on - strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.788 kb on - strand, within PP_5471 at 1677.788 kb on - strand, within PP_5471 at 1678.204 kb on - strand at 1678.208 kb on + strand at 1678.208 kb on + strand at 1678.208 kb on + strand at 1678.209 kb on - strand at 1678.209 kb on - strand at 1678.209 kb on - strand at 1678.315 kb on - strand, within PP_1473 at 1678.315 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.353 kb on + strand, within PP_1473 at 1678.443 kb on - strand, within PP_1473 at 1678.537 kb on + strand at 1678.537 kb on + strand at 1678.537 kb on + strand at 1678.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction pH 5 remove 1,675,716 + +0.1 1,675,716 + PP_1470 0.68 +0.1 1,675,733 + +0.1 1,675,742 + +0.1 1,675,887 + PP_1470 0.81 +0.1 1,675,890 + +0.1 1,676,143 + -1.2 1,677,606 - -1.3 1,677,666 + +3.0 1,677,666 + -0.1 1,677,681 - +2.1 1,677,681 - +0.9 1,677,681 - +1.1 1,677,706 - +1.3 1,677,706 - -1.9 1,677,750 + -2.0 1,677,750 + -2.9 1,677,750 + -0.3 1,677,750 + +1.1 1,677,751 - -0.3 1,677,751 - +0.8 1,677,751 - +0.9 1,677,751 - -1.7 1,677,751 - -0.1 1,677,788 - PP_5471 0.12 -0.3 1,677,788 - PP_5471 0.12 +1.1 1,678,204 - +0.1 1,678,208 + +3.6 1,678,208 + -0.8 1,678,208 + -0.2 1,678,209 - -0.5 1,678,209 - +1.2 1,678,209 - +1.4 1,678,315 - PP_1473 0.28 -0.6 1,678,315 - PP_1473 0.28 +0.3 1,678,351 - PP_1473 0.40 -0.5 1,678,351 - PP_1473 0.40 -3.5 1,678,351 - PP_1473 0.40 +0.1 1,678,353 + PP_1473 0.41 -0.2 1,678,443 - PP_1473 0.73 -0.0 1,678,537 + +0.1 1,678,537 + +1.5 1,678,537 + -3.7 1,678,538 - -1.9
Or see this region's nucleotide sequence