Experiment: pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1471 and PP_5471 are separated by 130 nucleotides PP_5471 and PP_1473 are separated by 79 nucleotides PP_1473 and PP_1474 are separated by 186 nucleotides
PP_1471: PP_1471 - Threonine synthase, at 1,676,198 to 1,677,607
_1471
PP_5471: PP_5471 - putative response regulator receiver protein, at 1,677,738 to 1,678,157
_5471
PP_1473: PP_1473 - conserved protein of unknown function, at 1,678,237 to 1,678,518
_1473
PP_1474: PP_1474 - conserved exported protein of unknown function, at 1,678,705 to 1,679,580
_1474
Position (kb)
1677
1678
1679 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1677.606 kb on - strand at 1677.666 kb on + strand at 1677.666 kb on + strand at 1677.681 kb on - strand at 1677.681 kb on - strand at 1677.681 kb on - strand at 1677.706 kb on - strand at 1677.706 kb on - strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.788 kb on - strand, within PP_5471 at 1677.788 kb on - strand, within PP_5471 at 1678.204 kb on - strand at 1678.208 kb on + strand at 1678.208 kb on + strand at 1678.208 kb on + strand at 1678.209 kb on - strand at 1678.209 kb on - strand at 1678.209 kb on - strand at 1678.315 kb on - strand, within PP_1473 at 1678.315 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.353 kb on + strand, within PP_1473 at 1678.443 kb on - strand, within PP_1473 at 1678.537 kb on + strand at 1678.537 kb on + strand at 1678.537 kb on + strand at 1678.538 kb on - strand at 1678.661 kb on + strand at 1678.661 kb on + strand at 1678.662 kb on - strand at 1678.662 kb on - strand at 1678.694 kb on - strand at 1678.706 kb on + strand at 1678.738 kb on + strand at 1678.738 kb on + strand at 1678.739 kb on - strand at 1678.739 kb on - strand at 1678.739 kb on - strand at 1678.742 kb on + strand at 1678.778 kb on + strand at 1678.778 kb on + strand at 1678.779 kb on - strand at 1678.779 kb on - strand at 1678.779 kb on - strand at 1678.810 kb on - strand, within PP_1474 at 1678.810 kb on - strand, within PP_1474 at 1678.908 kb on + strand, within PP_1474 at 1678.908 kb on + strand, within PP_1474 at 1678.919 kb on + strand, within PP_1474 at 1678.955 kb on + strand, within PP_1474 at 1678.956 kb on - strand, within PP_1474 at 1679.102 kb on + strand, within PP_1474 at 1679.103 kb on - strand, within PP_1474 at 1679.103 kb on - strand, within PP_1474 at 1679.103 kb on - strand, within PP_1474
Per-strain Table
Position Strand Gene LocusTag Fraction pH 4 remove 1,677,606 - +0.3 1,677,666 + +1.9 1,677,666 + +1.1 1,677,681 - +3.0 1,677,681 - -0.6 1,677,681 - -0.0 1,677,706 - -1.4 1,677,706 - -1.8 1,677,750 + -1.6 1,677,750 + +0.3 1,677,750 + -0.6 1,677,750 + +0.7 1,677,751 - -0.1 1,677,751 - -0.1 1,677,751 - -0.2 1,677,751 - -0.6 1,677,751 - +1.8 1,677,788 - PP_5471 0.12 +1.1 1,677,788 - PP_5471 0.12 +0.3 1,678,204 - -0.1 1,678,208 + -0.7 1,678,208 + +0.1 1,678,208 + +0.1 1,678,209 - -1.2 1,678,209 - -0.9 1,678,209 - +0.7 1,678,315 - PP_1473 0.28 +0.3 1,678,315 - PP_1473 0.28 -1.0 1,678,351 - PP_1473 0.40 +0.5 1,678,351 - PP_1473 0.40 -0.7 1,678,351 - PP_1473 0.40 +3.6 1,678,353 + PP_1473 0.41 -0.2 1,678,443 - PP_1473 0.73 -1.2 1,678,537 + +0.3 1,678,537 + +1.3 1,678,537 + -0.9 1,678,538 - -0.5 1,678,661 + -3.0 1,678,661 + -0.1 1,678,662 - +0.7 1,678,662 - +1.7 1,678,694 - +1.3 1,678,706 + +1.4 1,678,738 + +0.1 1,678,738 + -0.5 1,678,739 - -1.3 1,678,739 - -0.7 1,678,739 - +3.1 1,678,742 + -1.3 1,678,778 + +1.0 1,678,778 + -0.1 1,678,779 - -2.0 1,678,779 - -1.5 1,678,779 - +0.9 1,678,810 - PP_1474 0.12 +2.9 1,678,810 - PP_1474 0.12 -1.1 1,678,908 + PP_1474 0.23 +0.4 1,678,908 + PP_1474 0.23 -0.5 1,678,919 + PP_1474 0.24 +1.9 1,678,955 + PP_1474 0.29 +0.0 1,678,956 - PP_1474 0.29 +0.2 1,679,102 + PP_1474 0.45 -0.2 1,679,103 - PP_1474 0.45 +1.3 1,679,103 - PP_1474 0.45 -0.7 1,679,103 - PP_1474 0.45 +0.1
Or see this region's nucleotide sequence