Experiment: pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3835 and PP_3836 are separated by 84 nucleotides PP_3836 and PP_t45 are separated by 199 nucleotides PP_t45 and PP_3837 are separated by 104 nucleotides PP_3837 and PP_5634 are separated by 1 nucleotides PP_5634 and PP_3839 are separated by 435 nucleotides
PP_3835: PP_3835 - Sensory box histidine kinase, at 4,359,961 to 4,361,121
_3835
PP_3836: PP_3836 - conserved protein of unknown function, at 4,361,206 to 4,361,481
_3836
PP_t45: PP_t45 - tRNA-Leu, at 4,361,681 to 4,361,765
_t45
PP_3837: PP_3837 - inner membrane protein of unknown function, at 4,361,870 to 4,362,283
_3837
PP_5634: PP_5634 - conserved protein of unknown function, at 4,362,285 to 4,362,479
_5634
PP_3839: PP_3839 - short-chain alcohol dehydrogenase, at 4,362,915 to 4,363,925
_3839
Position (kb)
4361
4362
4363 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4360.965 kb on + strand, within PP_3835 at 4360.965 kb on + strand, within PP_3835 at 4361.033 kb on + strand at 4361.034 kb on - strand at 4361.034 kb on - strand at 4361.034 kb on - strand at 4361.034 kb on - strand at 4361.034 kb on - strand at 4361.042 kb on + strand at 4361.043 kb on - strand at 4361.161 kb on + strand at 4361.289 kb on + strand, within PP_3836 at 4361.289 kb on + strand, within PP_3836 at 4361.290 kb on - strand, within PP_3836 at 4361.290 kb on - strand, within PP_3836 at 4361.313 kb on + strand, within PP_3836 at 4361.314 kb on - strand, within PP_3836 at 4361.314 kb on - strand, within PP_3836 at 4361.314 kb on - strand, within PP_3836 at 4361.314 kb on - strand, within PP_3836 at 4361.314 kb on - strand, within PP_3836 at 4361.360 kb on + strand, within PP_3836 at 4361.361 kb on - strand, within PP_3836 at 4361.387 kb on + strand, within PP_3836 at 4361.388 kb on - strand, within PP_3836 at 4361.388 kb on - strand, within PP_3836 at 4361.437 kb on + strand, within PP_3836 at 4361.483 kb on - strand at 4361.488 kb on - strand at 4361.564 kb on - strand at 4361.576 kb on + strand at 4361.576 kb on + strand at 4361.577 kb on - strand at 4361.834 kb on - strand at 4361.876 kb on + strand at 4361.876 kb on + strand at 4361.876 kb on + strand at 4361.908 kb on + strand at 4361.908 kb on + strand at 4361.909 kb on - strand at 4361.993 kb on + strand, within PP_3837 at 4361.994 kb on - strand, within PP_3837 at 4361.994 kb on - strand, within PP_3837 at 4361.994 kb on - strand, within PP_3837 at 4361.994 kb on - strand, within PP_3837 at 4361.994 kb on - strand at 4362.089 kb on + strand, within PP_3837 at 4362.089 kb on + strand, within PP_3837 at 4362.089 kb on + strand, within PP_3837 at 4362.508 kb on + strand at 4362.508 kb on + strand at 4362.508 kb on + strand at 4362.509 kb on - strand at 4362.510 kb on + strand at 4362.510 kb on + strand at 4362.510 kb on + strand at 4362.510 kb on + strand at 4362.511 kb on - strand at 4362.511 kb on - strand at 4362.511 kb on - strand at 4362.548 kb on - strand at 4362.548 kb on - strand at 4362.629 kb on + strand at 4362.724 kb on + strand at 4362.724 kb on + strand at 4362.724 kb on + strand at 4362.725 kb on - strand at 4362.725 kb on - strand at 4362.725 kb on - strand at 4362.725 kb on - strand at 4362.791 kb on + strand at 4362.791 kb on + strand at 4362.791 kb on + strand at 4362.792 kb on - strand at 4362.792 kb on - strand at 4362.862 kb on + strand at 4362.862 kb on + strand at 4362.863 kb on - strand at 4362.863 kb on - strand at 4362.863 kb on - strand at 4362.863 kb on - strand at 4362.863 kb on - strand at 4362.899 kb on - strand at 4362.899 kb on - strand at 4362.958 kb on + strand at 4362.959 kb on - strand at 4363.078 kb on + strand, within PP_3839 at 4363.078 kb on + strand, within PP_3839 at 4363.078 kb on + strand, within PP_3839 at 4363.078 kb on + strand, within PP_3839 at 4363.079 kb on - strand, within PP_3839 at 4363.079 kb on - strand, within PP_3839 at 4363.094 kb on + strand, within PP_3839 at 4363.095 kb on - strand, within PP_3839 at 4363.170 kb on + strand, within PP_3839 at 4363.170 kb on + strand, within PP_3839 at 4363.171 kb on - strand, within PP_3839 at 4363.194 kb on + strand, within PP_3839
Per-strain Table
Position Strand Gene LocusTag Fraction pH 4 remove 4,360,965 + PP_3835 0.86 +0.6 4,360,965 + PP_3835 0.86 -2.6 4,361,033 + +1.2 4,361,034 - +3.1 4,361,034 - +0.9 4,361,034 - +0.0 4,361,034 - -0.0 4,361,034 - -1.2 4,361,042 + +0.0 4,361,043 - +1.5 4,361,161 + +0.1 4,361,289 + PP_3836 0.30 -1.4 4,361,289 + PP_3836 0.30 -1.5 4,361,290 - PP_3836 0.30 +0.1 4,361,290 - PP_3836 0.30 -2.4 4,361,313 + PP_3836 0.39 +0.8 4,361,314 - PP_3836 0.39 -0.3 4,361,314 - PP_3836 0.39 -0.4 4,361,314 - PP_3836 0.39 -0.8 4,361,314 - PP_3836 0.39 +0.7 4,361,314 - PP_3836 0.39 -1.2 4,361,360 + PP_3836 0.56 +1.7 4,361,361 - PP_3836 0.56 -3.3 4,361,387 + PP_3836 0.66 +4.9 4,361,388 - PP_3836 0.66 -1.3 4,361,388 - PP_3836 0.66 +1.4 4,361,437 + PP_3836 0.84 -0.8 4,361,483 - -1.6 4,361,488 - +2.2 4,361,564 - +3.7 4,361,576 + -3.4 4,361,576 + -0.3 4,361,577 - +3.2 4,361,834 - +0.0 4,361,876 + -3.0 4,361,876 + +0.9 4,361,876 + -2.5 4,361,908 + -1.3 4,361,908 + +1.0 4,361,909 - +3.8 4,361,993 + PP_3837 0.30 +1.3 4,361,994 - PP_3837 0.30 -0.8 4,361,994 - PP_3837 0.30 +1.4 4,361,994 - PP_3837 0.30 -3.7 4,361,994 - PP_3837 0.30 -1.4 4,361,994 - -1.4 4,362,089 + PP_3837 0.53 +2.2 4,362,089 + PP_3837 0.53 +1.7 4,362,089 + PP_3837 0.53 +0.2 4,362,508 + +0.2 4,362,508 + +0.2 4,362,508 + +0.1 4,362,509 - +0.2 4,362,510 + -0.6 4,362,510 + +0.5 4,362,510 + +0.2 4,362,510 + +0.9 4,362,511 - +0.2 4,362,511 - -0.8 4,362,511 - +0.8 4,362,548 - -0.5 4,362,548 - -0.5 4,362,629 + -2.7 4,362,724 + +1.2 4,362,724 + +0.4 4,362,724 + -1.7 4,362,725 - -0.5 4,362,725 - +0.9 4,362,725 - -0.7 4,362,725 - +1.8 4,362,791 + -0.2 4,362,791 + -0.1 4,362,791 + -0.1 4,362,792 - +1.4 4,362,792 - -1.5 4,362,862 + -1.5 4,362,862 + -0.6 4,362,863 - -0.1 4,362,863 - -2.1 4,362,863 - -1.4 4,362,863 - -2.3 4,362,863 - +0.6 4,362,899 - -0.6 4,362,899 - -1.0 4,362,958 + -0.2 4,362,959 - -0.8 4,363,078 + PP_3839 0.16 -1.0 4,363,078 + PP_3839 0.16 -0.3 4,363,078 + PP_3839 0.16 +3.2 4,363,078 + PP_3839 0.16 -1.1 4,363,079 - PP_3839 0.16 -0.7 4,363,079 - PP_3839 0.16 -0.6 4,363,094 + PP_3839 0.18 +2.3 4,363,095 - PP_3839 0.18 -0.4 4,363,170 + PP_3839 0.25 +0.9 4,363,170 + PP_3839 0.25 -0.9 4,363,171 - PP_3839 0.25 +1.3 4,363,194 + PP_3839 0.28 +0.1
Or see this region's nucleotide sequence