Experiment: pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2021 and PP_5496 are separated by 122 nucleotides PP_5496 and PP_2023 are separated by 132 nucleotides PP_2023 and PP_2024 overlap by 10 nucleotides
PP_2021: PP_2021 - L-alpha-hydroxyglutaric acid gamma-lactonase, at 2,294,325 to 2,295,455
_2021
PP_5496: PP_5496 - conserved exported protein of unknown function, at 2,295,578 to 2,295,754
_5496
PP_2023: PP_2023 - Glutathione S-transferase family protein, at 2,295,887 to 2,296,486
_2023
PP_2024: PP_2024 - Exonuclease SbcC, at 2,296,477 to 2,300,121
_2024
Position (kb)
2295
2296
2297 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2294.913 kb on + strand, within PP_2021 at 2294.913 kb on + strand, within PP_2021 at 2294.914 kb on - strand, within PP_2021 at 2294.914 kb on - strand, within PP_2021 at 2295.034 kb on - strand, within PP_2021 at 2295.186 kb on + strand, within PP_2021 at 2295.186 kb on + strand, within PP_2021 at 2295.186 kb on + strand, within PP_2021 at 2295.187 kb on - strand, within PP_2021 at 2295.367 kb on + strand at 2295.367 kb on + strand at 2295.367 kb on + strand at 2295.368 kb on - strand at 2295.368 kb on - strand at 2295.368 kb on - strand at 2295.472 kb on - strand at 2295.478 kb on + strand at 2295.570 kb on - strand at 2295.602 kb on - strand, within PP_5496 at 2295.686 kb on + strand, within PP_5496 at 2295.686 kb on + strand, within PP_5496 at 2295.687 kb on - strand, within PP_5496 at 2295.699 kb on - strand, within PP_5496 at 2296.057 kb on - strand, within PP_2023 at 2296.158 kb on + strand at 2296.158 kb on + strand, within PP_2023 at 2296.158 kb on + strand, within PP_2023 at 2296.158 kb on + strand, within PP_2023 at 2296.242 kb on - strand, within PP_2023 at 2296.562 kb on - strand at 2296.864 kb on + strand, within PP_2024 at 2296.864 kb on + strand, within PP_2024 at 2296.864 kb on + strand, within PP_2024 at 2296.865 kb on - strand, within PP_2024 at 2296.865 kb on - strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.904 kb on + strand, within PP_2024 at 2296.905 kb on - strand, within PP_2024
Per-strain Table
Position Strand Gene LocusTag Fraction pH 4 remove 2,294,913 + PP_2021 0.52 +1.8 2,294,913 + PP_2021 0.52 +0.5 2,294,914 - PP_2021 0.52 -0.6 2,294,914 - PP_2021 0.52 -0.1 2,295,034 - PP_2021 0.63 -0.4 2,295,186 + PP_2021 0.76 -0.7 2,295,186 + PP_2021 0.76 +0.2 2,295,186 + PP_2021 0.76 -0.1 2,295,187 - PP_2021 0.76 -0.8 2,295,367 + -0.8 2,295,367 + +0.4 2,295,367 + +1.4 2,295,368 - -0.1 2,295,368 - -2.0 2,295,368 - -3.9 2,295,472 - -0.9 2,295,478 + -0.0 2,295,570 - -0.2 2,295,602 - PP_5496 0.14 +0.3 2,295,686 + PP_5496 0.61 -0.8 2,295,686 + PP_5496 0.61 +1.4 2,295,687 - PP_5496 0.62 -0.7 2,295,699 - PP_5496 0.68 +0.5 2,296,057 - PP_2023 0.28 -1.8 2,296,158 + -0.8 2,296,158 + PP_2023 0.45 +0.2 2,296,158 + PP_2023 0.45 +0.8 2,296,158 + PP_2023 0.45 +1.2 2,296,242 - PP_2023 0.59 +1.2 2,296,562 - +0.7 2,296,864 + PP_2024 0.11 +0.2 2,296,864 + PP_2024 0.11 +0.7 2,296,864 + PP_2024 0.11 -0.1 2,296,865 - PP_2024 0.11 +1.8 2,296,865 - PP_2024 0.11 -0.8 2,296,904 + PP_2024 0.12 -1.1 2,296,904 + PP_2024 0.12 -1.4 2,296,904 + PP_2024 0.12 +0.2 2,296,905 - PP_2024 0.12 -2.8
Or see this region's nucleotide sequence