Experiment: pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0383 and PP_0384 are separated by 253 nucleotides PP_0384 and PP_0385 are separated by 187 nucleotides PP_0385 and PP_5445 are separated by 343 nucleotides PP_5445 and PP_t71 are separated by 52 nucleotides PP_t71 and PP_0386 are separated by 16 nucleotides
PP_0383: PP_0383 - Lysine 2-monooxygenase, at 463,414 to 465,096
_0383
PP_0384: PP_0384 - Transcriptional regulator, AsnC family, at 465,350 to 465,811
_0384
PP_0385: PP_0385 - putative serine acetyltransferase, at 465,999 to 466,553
_0385
PP_5445: PP_5445 - conserved protein of unknown function, at 466,897 to 467,067
_5445
PP_t71: PP_t71 - tRNA-Met, at 467,120 to 467,196
_t71
PP_0386: PP_0386 - Sensory box protein, at 467,213 to 470,956
_0386
Position (kb)
465
466
467 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 465.141 kb on + strand at 465.143 kb on + strand at 465.143 kb on + strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.166 kb on + strand at 465.167 kb on - strand at 465.184 kb on + strand at 465.188 kb on + strand at 465.189 kb on - strand at 465.189 kb on - strand at 465.189 kb on - strand at 465.189 kb on - strand at 465.214 kb on - strand at 465.241 kb on + strand at 465.242 kb on - strand at 465.271 kb on + strand at 465.271 kb on + strand at 465.271 kb on + strand at 465.272 kb on - strand at 465.307 kb on + strand at 465.308 kb on - strand at 465.486 kb on + strand, within PP_0384 at 465.486 kb on + strand, within PP_0384 at 465.487 kb on - strand, within PP_0384 at 465.487 kb on - strand, within PP_0384 at 465.487 kb on - strand at 465.504 kb on - strand, within PP_0384 at 465.591 kb on + strand, within PP_0384 at 465.591 kb on + strand, within PP_0384 at 465.592 kb on - strand, within PP_0384 at 465.592 kb on - strand, within PP_0384 at 465.592 kb on - strand, within PP_0384 at 465.592 kb on - strand, within PP_0384 at 465.632 kb on + strand, within PP_0384 at 465.640 kb on - strand, within PP_0384 at 465.649 kb on + strand, within PP_0384 at 465.649 kb on + strand, within PP_0384 at 465.649 kb on + strand, within PP_0384 at 465.649 kb on + strand, within PP_0384 at 465.650 kb on - strand, within PP_0384 at 465.650 kb on - strand, within PP_0384 at 465.650 kb on - strand, within PP_0384 at 465.823 kb on - strand at 465.841 kb on + strand at 465.874 kb on + strand at 465.874 kb on + strand at 465.875 kb on - strand at 465.875 kb on - strand at 465.875 kb on - strand at 466.067 kb on + strand, within PP_0385 at 466.149 kb on + strand, within PP_0385 at 466.149 kb on + strand, within PP_0385 at 466.218 kb on + strand, within PP_0385 at 466.299 kb on + strand, within PP_0385 at 466.300 kb on - strand, within PP_0385 at 466.374 kb on + strand, within PP_0385 at 466.375 kb on - strand, within PP_0385 at 466.456 kb on + strand, within PP_0385 at 466.457 kb on - strand, within PP_0385 at 466.512 kb on + strand at 466.564 kb on + strand at 466.565 kb on - strand at 466.565 kb on - strand at 466.616 kb on + strand at 466.616 kb on + strand at 466.688 kb on - strand at 466.728 kb on - strand at 466.756 kb on + strand at 466.865 kb on + strand at 466.865 kb on + strand at 466.903 kb on + strand at 466.904 kb on - strand at 466.904 kb on - strand at 466.922 kb on + strand, within PP_5445 at 466.923 kb on - strand, within PP_5445 at 467.006 kb on - strand, within PP_5445 at 467.051 kb on + strand at 467.051 kb on + strand at 467.052 kb on - strand at 467.070 kb on - strand at 467.202 kb on - strand at 467.202 kb on - strand at 467.215 kb on - strand at 467.215 kb on - strand at 467.217 kb on - strand at 467.262 kb on - strand at 467.262 kb on - strand at 467.262 kb on - strand at 467.317 kb on - strand at 467.317 kb on - strand at 467.317 kb on - strand at 467.520 kb on + strand at 467.521 kb on - strand at 467.521 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction pH 4 remove 465,141 + -1.5 465,143 + +0.6 465,143 + -0.5 465,144 - +2.3 465,144 - -0.8 465,144 - +0.5 465,166 + +0.4 465,167 - -0.6 465,184 + +0.0 465,188 + +0.4 465,189 - +2.3 465,189 - +0.6 465,189 - -1.0 465,189 - +1.7 465,214 - +3.0 465,241 + +2.4 465,242 - +2.7 465,271 + +0.8 465,271 + +1.1 465,271 + +2.0 465,272 - +0.8 465,307 + +2.2 465,308 - +2.4 465,486 + PP_0384 0.29 +0.4 465,486 + PP_0384 0.29 +1.4 465,487 - PP_0384 0.30 +0.8 465,487 - PP_0384 0.30 +0.6 465,487 - +0.4 465,504 - PP_0384 0.33 -1.0 465,591 + PP_0384 0.52 -0.1 465,591 + PP_0384 0.52 +3.0 465,592 - PP_0384 0.52 +4.3 465,592 - PP_0384 0.52 -0.2 465,592 - PP_0384 0.52 -1.6 465,592 - PP_0384 0.52 -1.7 465,632 + PP_0384 0.61 -0.7 465,640 - PP_0384 0.63 -0.4 465,649 + PP_0384 0.65 +0.4 465,649 + PP_0384 0.65 -0.3 465,649 + PP_0384 0.65 -2.9 465,649 + PP_0384 0.65 +0.4 465,650 - PP_0384 0.65 +0.4 465,650 - PP_0384 0.65 +1.6 465,650 - PP_0384 0.65 +0.5 465,823 - +0.8 465,841 + -0.8 465,874 + +0.9 465,874 + +2.4 465,875 - +0.4 465,875 - +3.2 465,875 - -2.0 466,067 + PP_0385 0.12 -0.6 466,149 + PP_0385 0.27 +0.6 466,149 + PP_0385 0.27 +2.4 466,218 + PP_0385 0.39 +0.0 466,299 + PP_0385 0.54 +2.0 466,300 - PP_0385 0.54 +3.0 466,374 + PP_0385 0.68 -2.4 466,375 - PP_0385 0.68 +1.1 466,456 + PP_0385 0.82 +1.1 466,457 - PP_0385 0.83 -0.6 466,512 + +2.0 466,564 + -0.5 466,565 - +0.6 466,565 - +1.6 466,616 + -1.0 466,616 + -0.1 466,688 - -0.3 466,728 - -0.1 466,756 + -0.6 466,865 + +1.3 466,865 + +0.4 466,903 + -0.2 466,904 - +0.8 466,904 - -1.1 466,922 + PP_5445 0.15 +0.1 466,923 - PP_5445 0.15 -0.9 467,006 - PP_5445 0.64 +2.7 467,051 + -3.0 467,051 + -3.0 467,052 - +1.4 467,070 - -2.0 467,202 - +0.4 467,202 - +2.4 467,215 - +3.6 467,215 - +1.6 467,217 - +0.4 467,262 - +0.4 467,262 - +0.0 467,262 - +0.9 467,317 - +0.4 467,317 - -0.3 467,317 - -0.6 467,520 + -0.6 467,521 - +0.2 467,521 - +2.7
Or see this region's nucleotide sequence