Experiment: carbon source Bisabolene 48.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0625 and PP_t07 are separated by 362 nucleotides PP_t07 and PP_t08 are separated by 4 nucleotides PP_t08 and PP_t09 are separated by 6 nucleotides PP_t09 and PP_t10 are separated by 46 nucleotides PP_t10 and PP_t11 are separated by 5 nucleotides PP_t11 and PP_0626 are separated by 190 nucleotides
PP_0625: PP_0625 - Chaperone protein ClpB, at 730,461 to 733,025
_0625
PP_t07: PP_t07 - tRNA-Asn, at 733,388 to 733,463
_t07
PP_t08: PP_t08 - tRNA-Pro, at 733,468 to 733,544
_t08
PP_t09: PP_t09 - tRNA-Lys, at 733,551 to 733,626
_t09
PP_t10: PP_t10 - tRNA-Pro, at 733,673 to 733,749
_t10
PP_t11: PP_t11 - tRNA-Lys, at 733,755 to 733,830
_t11
PP_0626: PP_0626 - NADH dehydrogenase, at 734,021 to 735,316
_0626
Position (kb) 733
734 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 732.777 kb on - strand at 732.889 kb on - strand at 733.179 kb on + strand at 733.181 kb on + strand at 733.181 kb on + strand at 733.181 kb on + strand at 733.475 kb on + strand at 733.475 kb on + strand at 733.629 kb on + strand at 733.681 kb on - strand, within PP_t10 at 733.681 kb on - strand, within PP_t10 at 733.763 kb on - strand, within PP_t11 at 733.763 kb on - strand, within PP_t11 at 733.775 kb on - strand, within PP_t11 at 733.775 kb on - strand, within PP_t11 at 733.775 kb on - strand, within PP_t11 at 733.912 kb on + strand at 733.913 kb on - strand at 733.913 kb on - strand at 733.930 kb on + strand at 733.931 kb on - strand at 733.931 kb on - strand at 733.934 kb on + strand at 733.934 kb on + strand at 733.934 kb on + strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.935 kb on - strand at 733.989 kb on - strand at 734.022 kb on + strand at 734.023 kb on - strand at 734.100 kb on + strand at 734.124 kb on + strand at 734.124 kb on + strand at 734.136 kb on + strand at 734.136 kb on + strand at 734.137 kb on - strand at 734.137 kb on - strand at 734.137 kb on - strand at 734.137 kb on - strand at 734.137 kb on - strand at 734.238 kb on + strand, within PP_0626 at 734.238 kb on + strand, within PP_0626 at 734.239 kb on - strand, within PP_0626 at 734.239 kb on - strand, within PP_0626 at 734.247 kb on + strand, within PP_0626 at 734.248 kb on - strand, within PP_0626 at 734.253 kb on + strand, within PP_0626 at 734.254 kb on - strand at 734.254 kb on - strand, within PP_0626 at 734.254 kb on - strand, within PP_0626 at 734.254 kb on - strand, within PP_0626 at 734.254 kb on - strand, within PP_0626 at 734.347 kb on + strand, within PP_0626 at 734.731 kb on - strand, within PP_0626 at 734.731 kb on - strand, within PP_0626 at 734.777 kb on + strand, within PP_0626 at 734.778 kb on - strand, within PP_0626 at 734.807 kb on + strand, within PP_0626
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Bisabolene 48.9 mM remove 732,777 - -2.1 732,889 - +0.1 733,179 + +0.1 733,181 + +0.1 733,181 + +0.1 733,181 + +0.1 733,475 + +2.1 733,475 + +3.1 733,629 + -2.2 733,681 - PP_t10 0.10 -2.0 733,681 - PP_t10 0.10 -4.0 733,763 - PP_t11 0.11 +0.1 733,763 - PP_t11 0.11 -0.9 733,775 - PP_t11 0.26 +2.4 733,775 - PP_t11 0.26 -0.9 733,775 - PP_t11 0.26 +1.1 733,912 + +0.0 733,913 - +0.1 733,913 - +0.8 733,930 + +0.7 733,931 - +0.1 733,931 - +3.7 733,934 + -0.8 733,934 + -1.1 733,934 + -0.5 733,935 - -1.4 733,935 - +0.9 733,935 - +0.2 733,935 - -1.4 733,935 - +0.1 733,935 - -1.9 733,935 - -0.1 733,935 - +1.3 733,989 - -2.7 734,022 + -0.3 734,023 - +1.9 734,100 + +0.1 734,124 + +0.5 734,124 + -0.1 734,136 + -1.2 734,136 + -0.9 734,137 - -1.5 734,137 - +0.4 734,137 - -0.5 734,137 - +0.9 734,137 - -1.2 734,238 + PP_0626 0.17 +0.1 734,238 + PP_0626 0.17 -3.0 734,239 - PP_0626 0.17 +0.1 734,239 - PP_0626 0.17 -0.6 734,247 + PP_0626 0.17 -1.2 734,248 - PP_0626 0.18 -0.6 734,253 + PP_0626 0.18 -1.4 734,254 - +0.1 734,254 - PP_0626 0.18 +0.6 734,254 - PP_0626 0.18 -4.0 734,254 - PP_0626 0.18 -0.6 734,254 - PP_0626 0.18 -1.8 734,347 + PP_0626 0.25 -0.7 734,731 - PP_0626 0.55 +0.1 734,731 - PP_0626 0.55 -0.8 734,777 + PP_0626 0.58 +0.1 734,778 - PP_0626 0.58 +2.4 734,807 + PP_0626 0.61 -0.9
Or see this region's nucleotide sequence