Experiment: Acetosyringone 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1325 and Atu1326 overlap by 4 nucleotides Atu1326 and Atu1327 are separated by 109 nucleotides Atu1327 and Atu1328 are separated by 131 nucleotides Atu1328 and Atu1329 are separated by 13 nucleotides
Atu1325: Atu1325 - acetyltransferase, at 1,314,168 to 1,314,665
Atu1325
Atu1326: Atu1326 - acetyltransferase, at 1,314,662 to 1,315,135
Atu1326
Atu1327: Atu1327 - NADH-ubiquinone oxidoreductase, at 1,315,245 to 1,315,643
Atu1327
Atu1328: Atu1328 - hypothetical protein, at 1,315,775 to 1,316,212
Atu1328
Atu1329: Atu1329 - leucyl/phenylalanyl-tRNA-protein transferase, at 1,316,226 to 1,316,840
Atu1329
Position (kb)
1315
1316 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1314.442 kb on - strand, within Atu1325 at 1314.492 kb on + strand, within Atu1325 at 1314.581 kb on - strand, within Atu1325 at 1314.813 kb on - strand, within Atu1326 at 1314.917 kb on - strand, within Atu1326 at 1314.939 kb on - strand, within Atu1326 at 1314.939 kb on - strand, within Atu1326 at 1315.017 kb on + strand, within Atu1326 at 1315.039 kb on + strand, within Atu1326 at 1315.496 kb on + strand, within Atu1327 at 1315.498 kb on + strand, within Atu1327 at 1315.663 kb on - strand at 1316.183 kb on + strand at 1316.381 kb on + strand, within Atu1329 at 1316.477 kb on + strand, within Atu1329 at 1316.489 kb on + strand, within Atu1329 at 1316.533 kb on + strand, within Atu1329 at 1316.596 kb on - strand, within Atu1329 at 1316.631 kb on - strand, within Atu1329
Per-strain Table
Position Strand Gene LocusTag Fraction Acetosyringone 5 mM remove 1,314,442 - Atu1325 0.55 +1.0 1,314,492 + Atu1325 0.65 -0.3 1,314,581 - Atu1325 0.83 +1.0 1,314,813 - Atu1326 0.32 +2.5 1,314,917 - Atu1326 0.54 +0.0 1,314,939 - Atu1326 0.58 -0.1 1,314,939 - Atu1326 0.58 -0.1 1,315,017 + Atu1326 0.75 -1.8 1,315,039 + Atu1326 0.80 +0.2 1,315,496 + Atu1327 0.63 -1.9 1,315,498 + Atu1327 0.63 -1.8 1,315,663 - -0.3 1,316,183 + -0.3 1,316,381 + Atu1329 0.25 +0.2 1,316,477 + Atu1329 0.41 +0.1 1,316,489 + Atu1329 0.43 +0.4 1,316,533 + Atu1329 0.50 +0.4 1,316,596 - Atu1329 0.60 -0.8 1,316,631 - Atu1329 0.66 -0.1
Or see this region's nucleotide sequence