Experiment: 3.75mM_PTB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01850 and DZA65_RS01855 are separated by 196 nucleotides DZA65_RS01855 and DZA65_RS22780 are separated by 230 nucleotides DZA65_RS22780 and DZA65_RS01860 are separated by 40 nucleotides
DZA65_RS01850: DZA65_RS01850 - carbohydrate porin, at 404,026 to 405,639
_RS01850
DZA65_RS01855: DZA65_RS01855 - aminoimidazole riboside kinase, at 405,836 to 406,759
_RS01855
DZA65_RS22780: DZA65_RS22780 - hypothetical protein, at 406,990 to 407,253
_RS22780
DZA65_RS01860: DZA65_RS01860 - type I-E CRISPR-associated protein Cse1/CasA, at 407,294 to 408,829
_RS01860
Position (kb) 405
406
407 Strain fitness (log2 ratio) -2
-1
0
1
2 at 404.852 kb on + strand, within DZA65_RS01850 at 404.852 kb on + strand, within DZA65_RS01850 at 404.852 kb on + strand, within DZA65_RS01850 at 404.852 kb on + strand, within DZA65_RS01850 at 404.853 kb on - strand, within DZA65_RS01850 at 404.853 kb on - strand, within DZA65_RS01850 at 404.853 kb on - strand, within DZA65_RS01850 at 404.853 kb on - strand, within DZA65_RS01850 at 404.853 kb on - strand, within DZA65_RS01850 at 404.896 kb on + strand, within DZA65_RS01850 at 405.047 kb on + strand, within DZA65_RS01850 at 405.047 kb on + strand, within DZA65_RS01850 at 405.047 kb on + strand, within DZA65_RS01850 at 405.047 kb on + strand, within DZA65_RS01850 at 405.047 kb on + strand, within DZA65_RS01850 at 405.047 kb on + strand, within DZA65_RS01850 at 405.047 kb on + strand, within DZA65_RS01850 at 405.048 kb on - strand, within DZA65_RS01850 at 405.048 kb on - strand, within DZA65_RS01850 at 405.048 kb on - strand, within DZA65_RS01850 at 405.048 kb on - strand, within DZA65_RS01850 at 405.048 kb on - strand, within DZA65_RS01850 at 405.048 kb on - strand, within DZA65_RS01850 at 405.048 kb on - strand, within DZA65_RS01850 at 405.048 kb on - strand, within DZA65_RS01850 at 405.137 kb on + strand, within DZA65_RS01850 at 405.137 kb on + strand, within DZA65_RS01850 at 405.137 kb on + strand, within DZA65_RS01850 at 405.137 kb on + strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.138 kb on - strand, within DZA65_RS01850 at 405.170 kb on + strand, within DZA65_RS01850 at 405.171 kb on - strand, within DZA65_RS01850 at 405.171 kb on - strand, within DZA65_RS01850 at 405.263 kb on + strand, within DZA65_RS01850 at 405.264 kb on - strand, within DZA65_RS01850 at 405.264 kb on - strand, within DZA65_RS01850 at 405.264 kb on - strand, within DZA65_RS01850 at 405.564 kb on + strand at 405.565 kb on - strand at 405.626 kb on + strand at 405.627 kb on - strand at 405.637 kb on + strand at 405.717 kb on + strand at 405.717 kb on + strand at 405.718 kb on - strand at 405.718 kb on - strand at 405.718 kb on - strand at 405.765 kb on + strand at 405.765 kb on + strand at 405.766 kb on - strand at 405.766 kb on - strand at 405.766 kb on - strand at 405.766 kb on - strand at 405.807 kb on + strand at 405.808 kb on - strand at 405.808 kb on - strand at 405.838 kb on - strand at 405.838 kb on - strand at 405.957 kb on + strand, within DZA65_RS01855 at 405.957 kb on + strand, within DZA65_RS01855 at 405.958 kb on - strand, within DZA65_RS01855 at 405.958 kb on - strand, within DZA65_RS01855 at 405.965 kb on - strand, within DZA65_RS01855 at 406.332 kb on - strand, within DZA65_RS01855 at 406.418 kb on + strand, within DZA65_RS01855 at 406.418 kb on + strand, within DZA65_RS01855 at 406.418 kb on + strand, within DZA65_RS01855 at 406.418 kb on + strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.543 kb on - strand, within DZA65_RS01855 at 406.739 kb on + strand at 406.745 kb on + strand at 406.745 kb on + strand at 406.746 kb on - strand at 406.746 kb on - strand at 406.750 kb on + strand at 406.751 kb on - strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.759 kb on + strand at 406.760 kb on - strand at 406.760 kb on - strand at 406.760 kb on - strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.763 kb on + strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.764 kb on - strand at 406.787 kb on + strand at 406.796 kb on + strand at 406.797 kb on - strand at 406.800 kb on + strand at 406.800 kb on + strand at 406.800 kb on + strand at 406.800 kb on + strand at 406.800 kb on + strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.801 kb on - strand at 406.926 kb on + strand at 406.933 kb on + strand at 406.933 kb on + strand at 406.934 kb on - strand at 406.934 kb on - strand at 406.934 kb on - strand at 406.934 kb on - strand at 406.934 kb on - strand at 406.959 kb on - strand at 406.974 kb on + strand at 406.974 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.987 kb on + strand at 406.988 kb on - strand at 406.988 kb on - strand at 406.988 kb on - strand at 406.988 kb on - strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.991 kb on + strand at 406.992 kb on - strand at 406.992 kb on - strand at 406.992 kb on - strand at 406.992 kb on - strand at 406.992 kb on - strand at 406.994 kb on + strand at 406.994 kb on + strand at 406.995 kb on - strand at 406.995 kb on - strand at 406.995 kb on - strand at 406.995 kb on - strand at 406.997 kb on + strand at 406.997 kb on + strand at 406.997 kb on + strand at 406.997 kb on + strand at 406.997 kb on + strand at 406.998 kb on - strand at 406.998 kb on - strand at 406.998 kb on - strand at 406.998 kb on - strand at 406.998 kb on - strand at 406.998 kb on - strand at 407.010 kb on + strand at 407.010 kb on + strand at 407.011 kb on - strand at 407.011 kb on - strand at 407.021 kb on + strand, within DZA65_RS22780 at 407.022 kb on - strand, within DZA65_RS22780 at 407.068 kb on - strand, within DZA65_RS22780 at 407.077 kb on - strand, within DZA65_RS22780 at 407.080 kb on - strand, within DZA65_RS22780 at 407.109 kb on - strand, within DZA65_RS22780 at 407.124 kb on + strand, within DZA65_RS22780 at 407.124 kb on + strand, within DZA65_RS22780 at 407.125 kb on - strand, within DZA65_RS22780 at 407.136 kb on - strand, within DZA65_RS22780 at 407.136 kb on - strand, within DZA65_RS22780 at 407.136 kb on - strand, within DZA65_RS22780 at 407.136 kb on - strand, within DZA65_RS22780 at 407.276 kb on + strand at 407.277 kb on - strand at 407.287 kb on + strand at 407.287 kb on + strand at 407.287 kb on + strand at 407.287 kb on + strand at 407.288 kb on - strand at 407.288 kb on - strand at 407.288 kb on - strand at 407.288 kb on - strand at 407.356 kb on + strand at 407.357 kb on - strand at 407.357 kb on - strand at 407.357 kb on - strand at 407.465 kb on + strand, within DZA65_RS01860 at 407.466 kb on - strand, within DZA65_RS01860 at 407.678 kb on - strand, within DZA65_RS01860 at 407.678 kb on - strand, within DZA65_RS01860 at 407.744 kb on + strand, within DZA65_RS01860 at 407.744 kb on + strand, within DZA65_RS01860 at 407.745 kb on - strand, within DZA65_RS01860
Per-strain Table
Position Strand Gene LocusTag Fraction 3.75mM_PTB remove 404,852 + DZA65_RS01850 0.51 +1.1 404,852 + DZA65_RS01850 0.51 +1.0 404,852 + DZA65_RS01850 0.51 +1.0 404,852 + DZA65_RS01850 0.51 -1.0 404,853 - DZA65_RS01850 0.51 +0.0 404,853 - DZA65_RS01850 0.51 -0.6 404,853 - DZA65_RS01850 0.51 +2.0 404,853 - DZA65_RS01850 0.51 +0.1 404,853 - DZA65_RS01850 0.51 -0.3 404,896 + DZA65_RS01850 0.54 +0.8 405,047 + DZA65_RS01850 0.63 -0.4 405,047 + DZA65_RS01850 0.63 -0.2 405,047 + DZA65_RS01850 0.63 +0.3 405,047 + DZA65_RS01850 0.63 +1.0 405,047 + DZA65_RS01850 0.63 -1.1 405,047 + DZA65_RS01850 0.63 +2.3 405,047 + DZA65_RS01850 0.63 +0.5 405,048 - DZA65_RS01850 0.63 +0.2 405,048 - DZA65_RS01850 0.63 +0.5 405,048 - DZA65_RS01850 0.63 +1.3 405,048 - DZA65_RS01850 0.63 +0.0 405,048 - DZA65_RS01850 0.63 +0.7 405,048 - DZA65_RS01850 0.63 -1.3 405,048 - DZA65_RS01850 0.63 +0.7 405,048 - DZA65_RS01850 0.63 -1.0 405,137 + DZA65_RS01850 0.69 -0.2 405,137 + DZA65_RS01850 0.69 -0.2 405,137 + DZA65_RS01850 0.69 -0.4 405,137 + DZA65_RS01850 0.69 +0.6 405,138 - DZA65_RS01850 0.69 -0.8 405,138 - DZA65_RS01850 0.69 -0.7 405,138 - DZA65_RS01850 0.69 -0.4 405,138 - DZA65_RS01850 0.69 -0.2 405,138 - DZA65_RS01850 0.69 -0.9 405,138 - DZA65_RS01850 0.69 -1.1 405,138 - DZA65_RS01850 0.69 -0.7 405,138 - DZA65_RS01850 0.69 +1.6 405,138 - DZA65_RS01850 0.69 +0.5 405,138 - DZA65_RS01850 0.69 -0.1 405,170 + DZA65_RS01850 0.71 -0.3 405,171 - DZA65_RS01850 0.71 -0.1 405,171 - DZA65_RS01850 0.71 -0.6 405,263 + DZA65_RS01850 0.77 +0.5 405,264 - DZA65_RS01850 0.77 -0.0 405,264 - DZA65_RS01850 0.77 +0.4 405,264 - DZA65_RS01850 0.77 +1.7 405,564 + +1.3 405,565 - -0.6 405,626 + +2.0 405,627 - +0.7 405,637 + -0.1 405,717 + -1.6 405,717 + -0.4 405,718 - +1.0 405,718 - -0.2 405,718 - +0.0 405,765 + +0.4 405,765 + -0.4 405,766 - +0.0 405,766 - +0.0 405,766 - -1.8 405,766 - -0.4 405,807 + +0.6 405,808 - -0.3 405,808 - -0.3 405,838 - -1.7 405,838 - -0.8 405,957 + DZA65_RS01855 0.13 -0.1 405,957 + DZA65_RS01855 0.13 +0.0 405,958 - DZA65_RS01855 0.13 -1.1 405,958 - DZA65_RS01855 0.13 -1.4 405,965 - DZA65_RS01855 0.14 -0.1 406,332 - DZA65_RS01855 0.54 +0.7 406,418 + DZA65_RS01855 0.63 -1.8 406,418 + DZA65_RS01855 0.63 -0.3 406,418 + DZA65_RS01855 0.63 +2.6 406,418 + DZA65_RS01855 0.63 -0.8 406,543 - DZA65_RS01855 0.77 -2.1 406,543 - DZA65_RS01855 0.77 +0.0 406,543 - DZA65_RS01855 0.77 -0.6 406,543 - DZA65_RS01855 0.77 -0.5 406,543 - DZA65_RS01855 0.77 +0.0 406,739 + +0.8 406,745 + +0.0 406,745 + -0.6 406,746 - +0.5 406,746 - +1.0 406,750 + +0.6 406,751 - -1.8 406,759 + +2.3 406,759 + -0.6 406,759 + +0.0 406,759 + +0.1 406,759 + -0.7 406,759 + -1.0 406,759 + -1.6 406,759 + +2.0 406,759 + -0.4 406,759 + +0.4 406,759 + +0.4 406,759 + -0.2 406,759 + +1.0 406,760 - -1.1 406,760 - -0.6 406,760 - +0.3 406,763 + +0.0 406,763 + +0.0 406,763 + -1.1 406,763 + -0.6 406,763 + +0.1 406,763 + +0.0 406,764 - -0.5 406,764 - +1.1 406,764 - -0.1 406,764 - +0.8 406,764 - +0.4 406,764 - +0.2 406,787 + +1.4 406,796 + -0.1 406,797 - +0.3 406,800 + -0.3 406,800 + +1.0 406,800 + -0.5 406,800 + -1.7 406,800 + -0.3 406,801 - -1.6 406,801 - +0.7 406,801 - +0.0 406,801 - -0.1 406,801 - -0.4 406,801 - +0.5 406,801 - -0.4 406,801 - -0.2 406,801 - -1.4 406,926 + -0.4 406,933 + -1.0 406,933 + +0.0 406,934 - -1.1 406,934 - -0.5 406,934 - -1.0 406,934 - +1.6 406,934 - -0.1 406,959 - -1.3 406,974 + +0.0 406,974 + -1.3 406,987 + -2.3 406,987 + -1.3 406,987 + -1.3 406,987 + -0.2 406,987 + -1.2 406,987 + -1.7 406,988 - -0.6 406,988 - +0.5 406,988 - -1.0 406,988 - -2.1 406,991 + -1.9 406,991 + +0.0 406,991 + +0.0 406,991 + -0.7 406,991 + +0.0 406,991 + -1.6 406,991 + +0.8 406,991 + +1.2 406,991 + -0.1 406,992 - -1.8 406,992 - -0.5 406,992 - -1.0 406,992 - +1.6 406,992 - -0.8 406,994 + -2.3 406,994 + +0.0 406,995 - -0.2 406,995 - +0.6 406,995 - -0.6 406,995 - -0.6 406,997 + -0.7 406,997 + +0.7 406,997 + -0.6 406,997 + -1.3 406,997 + +0.0 406,998 - +1.3 406,998 - -0.1 406,998 - +0.6 406,998 - -1.0 406,998 - +1.0 406,998 - -0.1 407,010 + +1.0 407,010 + -1.6 407,011 - -0.2 407,011 - -1.1 407,021 + DZA65_RS22780 0.12 -0.6 407,022 - DZA65_RS22780 0.12 +1.0 407,068 - DZA65_RS22780 0.30 -0.6 407,077 - DZA65_RS22780 0.33 +0.4 407,080 - DZA65_RS22780 0.34 -0.6 407,109 - DZA65_RS22780 0.45 -0.3 407,124 + DZA65_RS22780 0.51 +0.0 407,124 + DZA65_RS22780 0.51 +0.6 407,125 - DZA65_RS22780 0.51 -1.6 407,136 - DZA65_RS22780 0.55 -1.0 407,136 - DZA65_RS22780 0.55 -0.6 407,136 - DZA65_RS22780 0.55 +1.6 407,136 - DZA65_RS22780 0.55 +1.6 407,276 + -1.6 407,277 - -1.0 407,287 + +0.3 407,287 + -1.6 407,287 + +1.4 407,287 + -1.3 407,288 - +0.4 407,288 - +0.3 407,288 - -0.3 407,288 - +0.0 407,356 + +0.0 407,357 - -0.6 407,357 - -0.3 407,357 - -2.0 407,465 + DZA65_RS01860 0.11 -0.1 407,466 - DZA65_RS01860 0.11 -0.9 407,678 - DZA65_RS01860 0.25 -0.3 407,678 - DZA65_RS01860 0.25 +0.9 407,744 + DZA65_RS01860 0.29 -0.2 407,744 + DZA65_RS01860 0.29 +0.2 407,745 - DZA65_RS01860 0.29 +1.2
Or see this region's nucleotide sequence