Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10025 and DZA65_RS10030 overlap by 7 nucleotides DZA65_RS10030 and DZA65_RS10035 overlap by 19 nucleotides DZA65_RS10035 and DZA65_RS10040 are separated by 6 nucleotides DZA65_RS10040 and DZA65_RS10045 overlap by 1 nucleotides
DZA65_RS10025: DZA65_RS10025 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,123,237 to 2,123,851
_RS10025
DZA65_RS10030: DZA65_RS10030 - imidazole glycerol phosphate synthase subunit HisF, at 2,123,845 to 2,124,621
_RS10030
DZA65_RS10035: DZA65_RS10035 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,124,603 to 2,125,340
_RS10035
DZA65_RS10040: DZA65_RS10040 - imidazole glycerol phosphate synthase subunit HisH, at 2,125,347 to 2,125,937
_RS10040
DZA65_RS10045: DZA65_RS10045 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,125,937 to 2,127,004
_RS10045
Position (kb)
2124
2125
2126 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2123.620 kb on + strand at 2123.691 kb on + strand, within DZA65_RS10025 at 2123.727 kb on - strand, within DZA65_RS10025 at 2123.745 kb on + strand, within DZA65_RS10025 at 2123.745 kb on + strand, within DZA65_RS10025 at 2123.783 kb on + strand, within DZA65_RS10025 at 2123.783 kb on + strand, within DZA65_RS10025 at 2123.784 kb on - strand, within DZA65_RS10025 at 2123.784 kb on - strand at 2123.784 kb on - strand, within DZA65_RS10025 at 2123.878 kb on - strand at 2124.159 kb on + strand, within DZA65_RS10030 at 2124.188 kb on + strand, within DZA65_RS10030 at 2124.188 kb on + strand, within DZA65_RS10030 at 2124.256 kb on + strand, within DZA65_RS10030 at 2124.468 kb on + strand, within DZA65_RS10030 at 2124.473 kb on + strand, within DZA65_RS10030 at 2124.571 kb on + strand at 2124.571 kb on + strand at 2124.699 kb on + strand, within DZA65_RS10035 at 2124.794 kb on + strand, within DZA65_RS10035 at 2124.794 kb on + strand, within DZA65_RS10035 at 2124.870 kb on + strand, within DZA65_RS10035 at 2125.293 kb on + strand at 2125.348 kb on + strand at 2125.371 kb on + strand at 2125.885 kb on + strand at 2125.886 kb on - strand at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.164 kb on - strand, within DZA65_RS10045 at 2126.331 kb on + strand, within DZA65_RS10045 at 2126.332 kb on - strand, within DZA65_RS10045
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,123,620 + +0.2 2,123,691 + DZA65_RS10025 0.74 -0.5 2,123,727 - DZA65_RS10025 0.80 +0.8 2,123,745 + DZA65_RS10025 0.83 +1.8 2,123,745 + DZA65_RS10025 0.83 -2.0 2,123,783 + DZA65_RS10025 0.89 -0.5 2,123,783 + DZA65_RS10025 0.89 +0.3 2,123,784 - DZA65_RS10025 0.89 -0.4 2,123,784 - +1.4 2,123,784 - DZA65_RS10025 0.89 -0.5 2,123,878 - +0.3 2,124,159 + DZA65_RS10030 0.40 -2.0 2,124,188 + DZA65_RS10030 0.44 +0.8 2,124,188 + DZA65_RS10030 0.44 -1.1 2,124,256 + DZA65_RS10030 0.53 -0.8 2,124,468 + DZA65_RS10030 0.80 -0.6 2,124,473 + DZA65_RS10030 0.81 -0.8 2,124,571 + -0.8 2,124,571 + -1.5 2,124,699 + DZA65_RS10035 0.13 -2.0 2,124,794 + DZA65_RS10035 0.26 -1.2 2,124,794 + DZA65_RS10035 0.26 -1.5 2,124,870 + DZA65_RS10035 0.36 +0.2 2,125,293 + -0.2 2,125,348 + -0.2 2,125,371 + -0.2 2,125,885 + +0.5 2,125,886 - +1.4 2,126,163 + DZA65_RS10045 0.21 -0.6 2,126,163 + DZA65_RS10045 0.21 -1.1 2,126,163 + DZA65_RS10045 0.21 -1.5 2,126,164 - DZA65_RS10045 0.21 -0.6 2,126,331 + DZA65_RS10045 0.37 -1.0 2,126,332 - DZA65_RS10045 0.37 +0.8
Or see this region's nucleotide sequence