Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS03185 and DZA65_RS03190 are separated by 157 nucleotides DZA65_RS03190 and DZA65_RS03195 are separated by 47 nucleotides DZA65_RS03195 and DZA65_RS03200 are separated by 105 nucleotides DZA65_RS03200 and DZA65_RS03205 are separated by 8 nucleotides
DZA65_RS03185: DZA65_RS03185 - ribosome assembly RNA-binding protein YhbY, at 676,342 to 676,635
_RS03185
DZA65_RS03190: DZA65_RS03190 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 676,793 to 677,422
_RS03190
DZA65_RS03195: DZA65_RS03195 - ATP-dependent zinc metalloprotease FtsH, at 677,470 to 679,422
_RS03195
DZA65_RS03200: DZA65_RS03200 - dihydropteroate synthase, at 679,528 to 680,361
_RS03200
DZA65_RS03205: DZA65_RS03205 - phosphoglucosamine mutase, at 680,370 to 681,707
_RS03205
Position (kb) 677
678
679
680 Strain fitness (log2 ratio) -2
-1
0
1
2 at 676.535 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.535 kb on - strand, within DZA65_RS03185 at 676.623 kb on + strand at 676.676 kb on + strand at 676.693 kb on + strand at 676.693 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.754 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.758 kb on + strand at 676.764 kb on + strand at 676.764 kb on + strand at 676.779 kb on + strand at 677.420 kb on + strand at 677.420 kb on + strand at 677.420 kb on + strand at 677.423 kb on + strand at 679.311 kb on + strand at 679.311 kb on + strand at 679.311 kb on + strand at 679.389 kb on + strand at 679.389 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.392 kb on + strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.393 kb on - strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.420 kb on + strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.421 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.427 kb on - strand at 679.448 kb on + strand at 679.448 kb on + strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.449 kb on - strand at 679.484 kb on - strand at 679.484 kb on - strand at 679.515 kb on + strand at 679.516 kb on - strand at 679.516 kb on - strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.324 kb on + strand at 680.325 kb on - strand at 680.325 kb on - strand at 680.325 kb on - strand at 680.330 kb on + strand at 680.330 kb on + strand at 680.330 kb on + strand at 680.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 676,535 - DZA65_RS03185 0.66 -1.0 676,535 - DZA65_RS03185 0.66 +0.0 676,535 - DZA65_RS03185 0.66 +1.9 676,623 + -1.2 676,676 + -0.3 676,693 + -1.3 676,693 + -0.3 676,754 + -2.1 676,754 + -0.3 676,754 + -0.8 676,754 + -0.5 676,754 + -1.0 676,754 + -1.0 676,758 + +2.0 676,758 + +0.5 676,758 + -1.0 676,758 + -1.0 676,764 + +0.0 676,764 + -0.4 676,779 + -1.0 677,420 + -0.4 677,420 + +0.3 677,420 + +0.3 677,423 + -1.3 679,311 + +0.9 679,311 + -0.1 679,311 + +0.2 679,389 + -0.1 679,389 + -0.8 679,392 + -0.1 679,392 + -1.5 679,392 + +0.5 679,392 + +2.6 679,392 + -0.3 679,393 - -0.5 679,393 - -0.2 679,393 - -0.4 679,393 - +0.4 679,420 + -1.4 679,420 + -0.0 679,420 + +0.2 679,420 + -1.3 679,420 + -2.0 679,420 + +0.4 679,420 + +0.9 679,421 - +0.5 679,421 - +0.3 679,421 - -1.3 679,421 - +0.2 679,421 - +0.0 679,421 - +0.0 679,421 - -0.4 679,421 - -0.0 679,421 - -0.5 679,421 - -1.8 679,427 - +0.5 679,427 - -0.1 679,427 - -0.8 679,427 - -1.3 679,427 - -0.8 679,427 - +0.8 679,448 + -0.8 679,448 + +1.5 679,449 - -0.4 679,449 - +0.4 679,449 - -1.0 679,449 - -1.4 679,449 - +0.8 679,449 - -0.3 679,449 - -0.0 679,449 - -0.8 679,449 - +0.3 679,484 - -1.0 679,484 - -0.2 679,515 + +1.5 679,516 - -0.0 679,516 - +0.5 680,324 + -0.2 680,324 + -0.8 680,324 + -1.1 680,324 + -0.2 680,324 + +0.3 680,324 + +1.0 680,324 + -0.3 680,324 + -0.7 680,324 + +1.0 680,325 - -0.4 680,325 - +0.0 680,325 - -1.0 680,330 + -0.8 680,330 + +0.6 680,330 + +0.9 680,346 - +0.0
Or see this region's nucleotide sequence