Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS01510 and DZA65_RS01515 are separated by 49 nucleotides DZA65_RS01515 and DZA65_RS01520 are separated by 15 nucleotides DZA65_RS01520 and DZA65_RS01525 overlap by 23 nucleotides
DZA65_RS01510: DZA65_RS01510 - RNA polymerase factor sigma-54, at 331,473 to 332,906
_RS01510
DZA65_RS01515: DZA65_RS01515 - LPS export ABC transporter ATP-binding protein, at 332,956 to 333,681
_RS01515
DZA65_RS01520: DZA65_RS01520 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 333,697 to 334,275
_RS01520
DZA65_RS01525: DZA65_RS01525 - LPS export ABC transporter periplasmic protein LptC, at 334,253 to 334,822
_RS01525
Position (kb)
332
333
334 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 332.000 kb on - strand, within DZA65_RS01510 at 332.070 kb on - strand, within DZA65_RS01510 at 332.096 kb on - strand, within DZA65_RS01510 at 332.096 kb on - strand, within DZA65_RS01510 at 332.096 kb on - strand, within DZA65_RS01510 at 332.096 kb on - strand, within DZA65_RS01510 at 332.169 kb on + strand, within DZA65_RS01510 at 332.169 kb on + strand, within DZA65_RS01510 at 332.170 kb on - strand, within DZA65_RS01510 at 332.170 kb on - strand, within DZA65_RS01510 at 332.170 kb on - strand at 332.283 kb on - strand, within DZA65_RS01510 at 332.283 kb on - strand, within DZA65_RS01510 at 332.440 kb on - strand, within DZA65_RS01510 at 332.540 kb on - strand, within DZA65_RS01510 at 332.572 kb on + strand, within DZA65_RS01510 at 332.573 kb on - strand, within DZA65_RS01510 at 332.675 kb on + strand, within DZA65_RS01510 at 332.850 kb on + strand at 332.865 kb on - strand at 332.907 kb on - strand at 332.916 kb on + strand at 332.916 kb on + strand at 332.917 kb on - strand at 332.917 kb on - strand at 332.942 kb on - strand at 332.942 kb on - strand at 332.942 kb on - strand at 332.942 kb on - strand at 332.955 kb on - strand at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.366 kb on - strand, within DZA65_RS01525 at 334.407 kb on - strand, within DZA65_RS01525 at 334.413 kb on - strand, within DZA65_RS01525 at 334.413 kb on - strand, within DZA65_RS01525 at 334.413 kb on - strand, within DZA65_RS01525
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 332,000 - DZA65_RS01510 0.37 -0.4 332,070 - DZA65_RS01510 0.42 -0.4 332,096 - DZA65_RS01510 0.43 -0.4 332,096 - DZA65_RS01510 0.43 -0.6 332,096 - DZA65_RS01510 0.43 -2.0 332,096 - DZA65_RS01510 0.43 -0.5 332,169 + DZA65_RS01510 0.49 +1.1 332,169 + DZA65_RS01510 0.49 -0.9 332,170 - DZA65_RS01510 0.49 -0.3 332,170 - DZA65_RS01510 0.49 -0.2 332,170 - -1.2 332,283 - DZA65_RS01510 0.56 -0.4 332,283 - DZA65_RS01510 0.56 +0.1 332,440 - DZA65_RS01510 0.67 -0.6 332,540 - DZA65_RS01510 0.74 -0.6 332,572 + DZA65_RS01510 0.77 -0.4 332,573 - DZA65_RS01510 0.77 -0.3 332,675 + DZA65_RS01510 0.84 -0.1 332,850 + -0.3 332,865 - -0.7 332,907 - -0.7 332,916 + -1.7 332,916 + -0.3 332,917 - -0.9 332,917 - -1.4 332,942 - -1.4 332,942 - +0.6 332,942 - +0.5 332,942 - -1.4 332,955 - +0.1 334,366 - DZA65_RS01525 0.20 -1.4 334,366 - DZA65_RS01525 0.20 -2.3 334,366 - DZA65_RS01525 0.20 -2.3 334,366 - DZA65_RS01525 0.20 -2.2 334,366 - DZA65_RS01525 0.20 -2.2 334,366 - DZA65_RS01525 0.20 -1.7 334,407 - DZA65_RS01525 0.27 -2.0 334,413 - DZA65_RS01525 0.28 -3.3 334,413 - DZA65_RS01525 0.28 +2.5 334,413 - DZA65_RS01525 0.28 -3.4
Or see this region's nucleotide sequence