Strain Fitness in Escherichia coli BL21 around ECD_01442

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01440 and ECD_01441 are separated by 55 nucleotidesECD_01441 and ECD_01442 overlap by 8 nucleotidesECD_01442 and ECD_01443 overlap by 4 nucleotidesECD_01443 and ECD_01444 overlap by 4 nucleotides ECD_01440: ECD_01440 - lipoyl-dependent Cys-based peroxidase, hydroperoxide resistance; salt-shock inducible membrane protein; peroxiredoxin, at 1,512,093 to 1,512,524 _01440 ECD_01441: ECD_01441 - D,D-dipeptide ABC transporter ATPase, at 1,512,580 to 1,513,506 _01441 ECD_01442: ECD_01442 - D,D-dipeptide ABC transporter ATPase, at 1,513,499 to 1,514,485 _01442 ECD_01443: ECD_01443 - D,D-dipeptide ABC transporter permease, at 1,514,482 to 1,515,378 _01443 ECD_01444: ECD_01444 - D,D-dipeptide ABC transporter permease, at 1,515,375 to 1,516,397 _01444 Position (kb) 1513 1514 1515Strain fitness (log2 ratio) -1 0 1 2at 1512.629 kb on - strandat 1512.794 kb on + strand, within ECD_01441at 1513.180 kb on + strand, within ECD_01441at 1513.290 kb on - strand, within ECD_01441at 1513.508 kb on + strandat 1513.606 kb on - strand, within ECD_01442at 1513.730 kb on - strand, within ECD_01442at 1514.111 kb on + strand, within ECD_01442at 1514.868 kb on - strand, within ECD_01443at 1514.886 kb on + strand, within ECD_01443at 1514.974 kb on - strand, within ECD_01443at 1515.254 kb on - strand, within ECD_01443at 1515.419 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,512,629 - -0.9
1,512,794 + ECD_01441 0.23 +0.1
1,513,180 + ECD_01441 0.65 +0.0
1,513,290 - ECD_01441 0.77 -1.7
1,513,508 + +2.1
1,513,606 - ECD_01442 0.11 -0.2
1,513,730 - ECD_01442 0.23 -1.7
1,514,111 + ECD_01442 0.62 +0.4
1,514,868 - ECD_01443 0.43 -1.0
1,514,886 + ECD_01443 0.45 -0.3
1,514,974 - ECD_01443 0.55 -0.1
1,515,254 - ECD_01443 0.86 -0.5
1,515,419 - -0.4

Or see this region's nucleotide sequence