Strain Fitness in Escherichia coli BL21 around ECD_01382

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01381 and ECD_01382 overlap by 80 nucleotidesECD_01382 and ECD_01383 are separated by 15 nucleotidesECD_01383 and ECD_01384 are separated by 62 nucleotidesECD_01384 and ECD_01385 overlap by 9 nucleotides ECD_01381: ECD_01381 - OPG biosynthetic periplasmic protein, at 1,450,948 to 1,452,573 _01381 ECD_01382: ECD_01382 - hypothetical protein, at 1,452,494 to 1,452,697 _01382 ECD_01383: ECD_01383 - DUF465 family protein, at 1,452,713 to 1,452,937 _01383 ECD_01384: ECD_01384 - ribosomal-protein-L7/L12-serine acetyltransferase, at 1,453,000 to 1,453,539 _01384 ECD_01385: ECD_01385 - uncharacterized protein, at 1,453,531 to 1,454,511 _01385 Position (kb) 1452 1453Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1451.527 kb on + strand, within ECD_01381at 1451.528 kb on - strand, within ECD_01381at 1451.599 kb on - strand, within ECD_01381at 1451.707 kb on + strand, within ECD_01381at 1451.707 kb on + strand, within ECD_01381at 1451.973 kb on + strand, within ECD_01381at 1452.388 kb on - strand, within ECD_01381at 1452.469 kb on + strandat 1452.469 kb on + strandat 1452.469 kb on + strandat 1452.470 kb on - strandat 1452.517 kb on + strandat 1452.547 kb on + strandat 1452.572 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,451,527 + ECD_01381 0.36 +1.0
1,451,528 - ECD_01381 0.36 -0.2
1,451,599 - ECD_01381 0.40 +0.4
1,451,707 + ECD_01381 0.47 +0.4
1,451,707 + ECD_01381 0.47 +1.1
1,451,973 + ECD_01381 0.63 +2.3
1,452,388 - ECD_01381 0.89 -0.5
1,452,469 + -3.5
1,452,469 + -0.7
1,452,469 + -1.1
1,452,470 - +0.5
1,452,517 + +0.7
1,452,547 + +0.0
1,452,572 - -0.7

Or see this region's nucleotide sequence