Strain Fitness in Escherichia coli BW25113 around b1862

Experiment: LB Anaerobic with Norfloxacin 0.00000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntruvB and ruvA are separated by 8 nucleotidesruvA and yobI are separated by 138 nucleotidesyobI and yebB are separated by 70 nucleotidesyebB and ruvC are separated by 1 nucleotidesruvC and yebC are separated by 34 nucleotides b1860: ruvB - Holliday junction DNA helicase B (NCBI), at 1,942,370 to 1,943,380 ruvB b1861: ruvA - Holliday junction DNA helicase motor protein (NCBI), at 1,943,389 to 1,944,000 ruvA b4677: yobI - expressed protein (RefSeq), at 1,944,139 to 1,944,204 yobI b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877 yebB b1863: ruvC - Holliday junction resolvase (NCBI), at 1,944,879 to 1,945,400 ruvC b1864: yebC - hypothetical protein (NCBI), at 1,945,435 to 1,946,175 yebC Position (kb) 1944 1945Strain fitness (log2 ratio) -2 -1 0 1 2at 1943.892 kb on + strand, within ruvAat 1943.919 kb on - strand, within ruvAat 1943.934 kb on - strand, within ruvAat 1943.990 kb on - strandat 1943.996 kb on + strandat 1943.996 kb on + strandat 1944.021 kb on + strandat 1944.102 kb on + strandat 1944.102 kb on + strandat 1944.137 kb on + strandat 1944.333 kb on - strandat 1944.333 kb on - strandat 1944.436 kb on - strand, within yebBat 1944.511 kb on - strand, within yebBat 1944.560 kb on - strand, within yebBat 1944.582 kb on + strand, within yebBat 1944.622 kb on + strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.625 kb on - strand, within yebBat 1944.669 kb on - strand, within yebBat 1944.674 kb on - strand, within yebBat 1944.723 kb on - strand, within yebBat 1944.818 kb on + strandat 1944.818 kb on + strandat 1944.826 kb on - strandat 1944.826 kb on - strandat 1944.829 kb on + strandat 1944.846 kb on - strandat 1944.885 kb on - strandat 1944.885 kb on - strandat 1944.895 kb on - strandat 1944.908 kb on + strandat 1944.916 kb on - strandat 1945.037 kb on - strand, within ruvCat 1945.078 kb on - strand, within ruvCat 1945.219 kb on + strand, within ruvCat 1945.255 kb on - strand, within ruvCat 1945.260 kb on - strand, within ruvCat 1945.307 kb on + strand, within ruvCat 1945.323 kb on - strand, within ruvCat 1945.396 kb on - strandat 1945.455 kb on + strandat 1945.524 kb on - strand, within yebCat 1945.557 kb on - strand, within yebCat 1945.557 kb on - strand, within yebCat 1945.623 kb on - strand, within yebCat 1945.623 kb on - strand, within yebCat 1945.640 kb on - strand, within yebCat 1945.640 kb on - strand, within yebCat 1945.652 kb on + strand, within yebCat 1945.663 kb on - strand, within yebCat 1945.726 kb on - strand, within yebC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Norfloxacin 0.00000015625 mM
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1,943,892 + ruvA b1861 0.82 -0.1
1,943,919 - ruvA b1861 0.87 +0.5
1,943,934 - ruvA b1861 0.89 +0.5
1,943,990 - +0.5
1,943,996 + +0.5
1,943,996 + +0.5
1,944,021 + -0.4
1,944,102 + +0.9
1,944,102 + -1.6
1,944,137 + -0.1
1,944,333 - -0.2
1,944,333 - +0.4
1,944,436 - yebB b1862 0.27 +1.5
1,944,511 - yebB b1862 0.39 +1.5
1,944,560 - yebB b1862 0.47 -1.3
1,944,582 + yebB b1862 0.51 -0.8
1,944,622 + yebB b1862 0.58 +1.5
1,944,625 - yebB b1862 0.58 -0.3
1,944,625 - yebB b1862 0.58 -1.0
1,944,625 - yebB b1862 0.58 +0.5
1,944,669 - yebB b1862 0.65 -0.1
1,944,674 - yebB b1862 0.66 -0.1
1,944,723 - yebB b1862 0.74 -0.5
1,944,818 + -1.3
1,944,818 + -0.1
1,944,826 - +1.9
1,944,826 - -1.1
1,944,829 + -2.6
1,944,846 - -0.5
1,944,885 - +0.7
1,944,885 - -1.6
1,944,895 - -2.4
1,944,908 + +0.5
1,944,916 - -0.6
1,945,037 - ruvC b1863 0.30 -0.8
1,945,078 - ruvC b1863 0.38 -0.1
1,945,219 + ruvC b1863 0.65 -0.1
1,945,255 - ruvC b1863 0.72 +1.5
1,945,260 - ruvC b1863 0.73 -1.5
1,945,307 + ruvC b1863 0.82 -0.1
1,945,323 - ruvC b1863 0.85 -1.3
1,945,396 - -0.1
1,945,455 + -1.9
1,945,524 - yebC b1864 0.12 -0.2
1,945,557 - yebC b1864 0.16 -0.3
1,945,557 - yebC b1864 0.16 -0.4
1,945,623 - yebC b1864 0.25 +0.6
1,945,623 - yebC b1864 0.25 -0.2
1,945,640 - yebC b1864 0.28 -0.1
1,945,640 - yebC b1864 0.28 +0.5
1,945,652 + yebC b1864 0.29 -2.6
1,945,663 - yebC b1864 0.31 -0.1
1,945,726 - yebC b1864 0.39 -0.0

Or see this region's nucleotide sequence