Strain Fitness in Escherichia coli BW25113 around b1616

Experiment: LB Anaerobic with Norfloxacin 0.00000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuidC and uidB are separated by 38 nucleotidesuidB and uidA overlap by 4 nucleotides b1615: uidC - membrane-associated protein (VIMSS), at 1,689,610 to 1,690,875 uidC b1616: uidB - glucuronide transporter (NCBI), at 1,690,914 to 1,692,287 uidB b1617: uidA - beta-D-glucuronidase (NCBI), at 1,692,284 to 1,694,095 uidA Position (kb) 1690 1691 1692 1693Strain fitness (log2 ratio) -2 -1 0 1at 1690.093 kb on - strand, within uidCat 1690.093 kb on - strand, within uidCat 1690.166 kb on + strand, within uidCat 1690.208 kb on + strand, within uidCat 1690.209 kb on + strand, within uidCat 1690.291 kb on + strand, within uidCat 1690.308 kb on + strand, within uidCat 1690.368 kb on + strand, within uidCat 1690.701 kb on - strand, within uidCat 1690.702 kb on - strand, within uidCat 1691.059 kb on - strand, within uidBat 1691.059 kb on - strand, within uidBat 1691.059 kb on - strand, within uidBat 1691.084 kb on - strand, within uidBat 1691.152 kb on - strand, within uidBat 1691.197 kb on - strand, within uidBat 1691.207 kb on - strand, within uidBat 1691.244 kb on - strand, within uidBat 1691.458 kb on + strand, within uidBat 1691.458 kb on + strand, within uidBat 1691.486 kb on + strand, within uidBat 1691.489 kb on - strand, within uidBat 1691.489 kb on - strand, within uidBat 1691.699 kb on - strand, within uidBat 1691.699 kb on - strand, within uidBat 1691.787 kb on - strand, within uidBat 1691.793 kb on - strand, within uidBat 1691.867 kb on - strand, within uidBat 1691.898 kb on + strand, within uidBat 1691.898 kb on + strand, within uidBat 1691.963 kb on + strand, within uidBat 1691.963 kb on + strand, within uidBat 1691.976 kb on + strand, within uidBat 1692.031 kb on + strand, within uidBat 1692.031 kb on + strand, within uidBat 1692.089 kb on - strand, within uidBat 1692.195 kb on - strandat 1692.710 kb on - strand, within uidAat 1692.732 kb on + strand, within uidAat 1693.129 kb on + strand, within uidAat 1693.155 kb on - strand, within uidAat 1693.171 kb on - strand, within uidAat 1693.171 kb on - strand, within uidAat 1693.245 kb on - strand, within uidAat 1693.245 kb on - strand, within uidA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Norfloxacin 0.00000015625 mM
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1,690,093 - uidC b1615 0.38 -0.3
1,690,093 - uidC b1615 0.38 +0.6
1,690,166 + uidC b1615 0.44 -0.1
1,690,208 + uidC b1615 0.47 +0.5
1,690,209 + uidC b1615 0.47 +1.5
1,690,291 + uidC b1615 0.54 +1.5
1,690,308 + uidC b1615 0.55 +1.5
1,690,368 + uidC b1615 0.60 +1.5
1,690,701 - uidC b1615 0.86 +1.5
1,690,702 - uidC b1615 0.86 +1.5
1,691,059 - uidB b1616 0.11 +0.5
1,691,059 - uidB b1616 0.11 -1.9
1,691,059 - uidB b1616 0.11 +0.1
1,691,084 - uidB b1616 0.12 +0.5
1,691,152 - uidB b1616 0.17 -0.4
1,691,197 - uidB b1616 0.21 -2.5
1,691,207 - uidB b1616 0.21 +0.9
1,691,244 - uidB b1616 0.24 +0.5
1,691,458 + uidB b1616 0.40 -0.1
1,691,458 + uidB b1616 0.40 -1.7
1,691,486 + uidB b1616 0.42 -0.5
1,691,489 - uidB b1616 0.42 -0.2
1,691,489 - uidB b1616 0.42 -0.8
1,691,699 - uidB b1616 0.57 -0.9
1,691,699 - uidB b1616 0.57 -2.5
1,691,787 - uidB b1616 0.64 -0.1
1,691,793 - uidB b1616 0.64 -0.0
1,691,867 - uidB b1616 0.69 -0.4
1,691,898 + uidB b1616 0.72 -1.7
1,691,898 + uidB b1616 0.72 -0.9
1,691,963 + uidB b1616 0.76 -0.4
1,691,963 + uidB b1616 0.76 -0.4
1,691,976 + uidB b1616 0.77 -1.0
1,692,031 + uidB b1616 0.81 +0.1
1,692,031 + uidB b1616 0.81 +0.4
1,692,089 - uidB b1616 0.86 -1.5
1,692,195 - +0.6
1,692,710 - uidA b1617 0.24 +0.4
1,692,732 + uidA b1617 0.25 -0.3
1,693,129 + uidA b1617 0.47 +1.7
1,693,155 - uidA b1617 0.48 +0.7
1,693,171 - uidA b1617 0.49 -0.4
1,693,171 - uidA b1617 0.49 -0.5
1,693,245 - uidA b1617 0.53 -0.1
1,693,245 - uidA b1617 0.53 +0.6

Or see this region's nucleotide sequence