Strain Fitness in Escherichia coli BW25113 around b1154

Experiment: LB Anaerobic with Norfloxacin 0.00000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntymfP and ymfQ overlap by 10 nucleotidesymfQ and ycfK are separated by 3 nucleotidesycfK and ymfS are separated by 1 nucleotidesymfS and tfaE overlap by 29 nucleotidestfaE and stfE overlap by 1 nucleotides b1152: ymfP - orf, hypothetical protein (VIMSS), at 1,205,366 to 1,206,145 ymfP b1153: ymfQ - e14 prophage; conserved protein (NCBI), at 1,206,136 to 1,206,720 ymfQ b1154: ycfK - e14 prophage; predicted protein (NCBI), at 1,206,724 to 1,207,353 ycfK b1155: ymfS - e14 prophage; predicted protein (NCBI), at 1,207,355 to 1,207,768 ymfS b1156: tfaE - e14 prophage; predicted tail fiber assembly protein (NCBI), at 1,207,740 to 1,208,342 tfaE b1157: stfE - no description, at 1,208,342 to 1,208,842 stfE Position (kb) 1206 1207 1208Strain fitness (log2 ratio) -1 0 1 2 3at 1205.740 kb on - strand, within ymfPat 1205.869 kb on - strand, within ymfPat 1206.162 kb on - strandat 1206.273 kb on + strand, within ymfQat 1206.457 kb on + strand, within ymfQat 1206.660 kb on - strand, within ymfQat 1206.672 kb on - strandat 1207.069 kb on + strand, within ycfKat 1207.070 kb on + strand, within ycfKat 1207.076 kb on + strand, within ycfKat 1207.084 kb on - strand, within ycfKat 1207.097 kb on + strand, within ycfKat 1207.147 kb on + strand, within ycfKat 1207.155 kb on + strand, within ycfKat 1207.156 kb on + strand, within ycfKat 1207.164 kb on + strand, within ycfKat 1207.169 kb on + strand, within ycfKat 1207.177 kb on + strand, within ycfKat 1207.177 kb on + strand, within ycfKat 1207.192 kb on + strand, within ycfKat 1207.246 kb on + strand, within ycfKat 1207.248 kb on + strand, within ycfKat 1207.248 kb on + strand, within ycfKat 1207.258 kb on + strand, within ycfKat 1207.258 kb on + strand, within ycfKat 1207.260 kb on + strand, within ycfKat 1207.267 kb on + strand, within ycfKat 1207.270 kb on + strand, within ycfKat 1207.291 kb on + strandat 1207.291 kb on + strandat 1207.339 kb on + strandat 1207.390 kb on + strandat 1207.390 kb on + strandat 1207.540 kb on - strand, within ymfSat 1207.545 kb on - strand, within ymfSat 1207.563 kb on + strand, within ymfSat 1207.605 kb on + strand, within ymfSat 1207.617 kb on + strand, within ymfSat 1207.617 kb on + strand, within ymfSat 1207.624 kb on - strand, within ymfSat 1207.624 kb on - strand, within ymfSat 1207.626 kb on + strand, within ymfSat 1207.633 kb on + strand, within ymfSat 1207.649 kb on - strand, within ymfSat 1207.681 kb on + strand, within ymfSat 1207.703 kb on - strand, within ymfSat 1207.746 kb on - strandat 1207.746 kb on - strandat 1207.746 kb on - strandat 1207.746 kb on - strandat 1207.772 kb on - strandat 1207.780 kb on + strandat 1207.780 kb on + strandat 1207.792 kb on + strandat 1207.803 kb on - strand, within tfaEat 1207.815 kb on + strand, within tfaEat 1207.815 kb on + strand, within tfaEat 1207.859 kb on - strand, within tfaEat 1207.959 kb on + strand, within tfaEat 1207.962 kb on - strand, within tfaEat 1207.964 kb on - strand, within tfaEat 1207.967 kb on + strand, within tfaEat 1207.972 kb on + strand, within tfaEat 1208.100 kb on - strand, within tfaEat 1208.167 kb on - strand, within tfaEat 1208.167 kb on - strand, within tfaEat 1208.168 kb on - strand, within tfaEat 1208.168 kb on - strand, within tfaEat 1208.170 kb on + strand, within tfaEat 1208.170 kb on + strand, within tfaEat 1208.172 kb on + strand, within tfaEat 1208.177 kb on + strand, within tfaEat 1208.178 kb on - strand, within tfaEat 1208.178 kb on - strand, within tfaEat 1208.180 kb on - strand, within tfaEat 1208.180 kb on - strand, within tfaEat 1208.184 kb on + strand, within tfaEat 1208.185 kb on - strand, within tfaEat 1208.187 kb on - strand, within tfaEat 1208.194 kb on + strand, within tfaEat 1208.194 kb on + strand, within tfaEat 1208.194 kb on + strand, within tfaEat 1208.352 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Norfloxacin 0.00000015625 mM
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1,205,740 - ymfP b1152 0.48 -0.2
1,205,869 - ymfP b1152 0.64 +1.0
1,206,162 - +1.2
1,206,273 + ymfQ b1153 0.23 +1.4
1,206,457 + ymfQ b1153 0.55 +0.3
1,206,660 - ymfQ b1153 0.90 +1.0
1,206,672 - +1.7
1,207,069 + ycfK b1154 0.55 +0.4
1,207,070 + ycfK b1154 0.55 +1.2
1,207,076 + ycfK b1154 0.56 +2.0
1,207,084 - ycfK b1154 0.57 -0.2
1,207,097 + ycfK b1154 0.59 +1.8
1,207,147 + ycfK b1154 0.67 +0.9
1,207,155 + ycfK b1154 0.68 +1.5
1,207,156 + ycfK b1154 0.69 +0.1
1,207,164 + ycfK b1154 0.70 +1.7
1,207,169 + ycfK b1154 0.71 +0.8
1,207,177 + ycfK b1154 0.72 -0.1
1,207,177 + ycfK b1154 0.72 -0.2
1,207,192 + ycfK b1154 0.74 +0.3
1,207,246 + ycfK b1154 0.83 +2.5
1,207,248 + ycfK b1154 0.83 -0.3
1,207,248 + ycfK b1154 0.83 +0.4
1,207,258 + ycfK b1154 0.85 +0.7
1,207,258 + ycfK b1154 0.85 +1.1
1,207,260 + ycfK b1154 0.85 +0.8
1,207,267 + ycfK b1154 0.86 +1.8
1,207,270 + ycfK b1154 0.87 +0.6
1,207,291 + +0.7
1,207,291 + +1.1
1,207,339 + +0.7
1,207,390 + +1.2
1,207,390 + +1.1
1,207,540 - ymfS b1155 0.45 +1.7
1,207,545 - ymfS b1155 0.46 +0.9
1,207,563 + ymfS b1155 0.50 +0.7
1,207,605 + ymfS b1155 0.60 +1.0
1,207,617 + ymfS b1155 0.63 +1.2
1,207,617 + ymfS b1155 0.63 +0.6
1,207,624 - ymfS b1155 0.65 -0.9
1,207,624 - ymfS b1155 0.65 +2.7
1,207,626 + ymfS b1155 0.65 +1.0
1,207,633 + ymfS b1155 0.67 +0.7
1,207,649 - ymfS b1155 0.71 +0.7
1,207,681 + ymfS b1155 0.79 +1.1
1,207,703 - ymfS b1155 0.84 +1.7
1,207,746 - +0.9
1,207,746 - +1.9
1,207,746 - +0.6
1,207,746 - +1.4
1,207,772 - +0.1
1,207,780 + +1.4
1,207,780 + +2.3
1,207,792 + +2.1
1,207,803 - tfaE b1156 0.10 +0.1
1,207,815 + tfaE b1156 0.12 -0.8
1,207,815 + tfaE b1156 0.12 -1.1
1,207,859 - tfaE b1156 0.20 +0.9
1,207,959 + tfaE b1156 0.36 +0.1
1,207,962 - tfaE b1156 0.37 -0.4
1,207,964 - tfaE b1156 0.37 +0.1
1,207,967 + tfaE b1156 0.38 -0.3
1,207,972 + tfaE b1156 0.38 +0.7
1,208,100 - tfaE b1156 0.60 +0.1
1,208,167 - tfaE b1156 0.71 +0.9
1,208,167 - tfaE b1156 0.71 +2.0
1,208,168 - tfaE b1156 0.71 +3.0
1,208,168 - tfaE b1156 0.71 +1.1
1,208,170 + tfaE b1156 0.71 +1.7
1,208,170 + tfaE b1156 0.71 +0.7
1,208,172 + tfaE b1156 0.72 +1.7
1,208,177 + tfaE b1156 0.72 +1.7
1,208,178 - tfaE b1156 0.73 +0.7
1,208,178 - tfaE b1156 0.73 -0.6
1,208,180 - tfaE b1156 0.73 +0.7
1,208,180 - tfaE b1156 0.73 +1.7
1,208,184 + tfaE b1156 0.74 +1.7
1,208,185 - tfaE b1156 0.74 +1.1
1,208,187 - tfaE b1156 0.74 +0.7
1,208,194 + tfaE b1156 0.75 +1.7
1,208,194 + tfaE b1156 0.75 +1.7
1,208,194 + tfaE b1156 0.75 +1.7
1,208,352 - -0.3

Or see this region's nucleotide sequence