Strain Fitness in Escherichia coli BW25113 around b0973

Experiment: LB Anaerobic with Ampicillin 0.00000195313 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyaA and hyaB overlap by 4 nucleotideshyaB and hyaC are separated by 18 nucleotideshyaC and hyaD overlap by 4 nucleotides b0972: hyaA - hydrogenase 1, small subunit (NCBI), at 1,031,362 to 1,032,480 hyaA b0973: hyaB - hydrogenase 1, large subunit (NCBI), at 1,032,477 to 1,034,270 hyaB b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996 hyaC b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580 hyaD Position (kb) 1032 1033 1034 1035Strain fitness (log2 ratio) -1 0 1 2 3at 1031.478 kb on + strand, within hyaAat 1031.511 kb on + strand, within hyaAat 1031.552 kb on + strand, within hyaAat 1031.702 kb on - strand, within hyaAat 1031.702 kb on - strand, within hyaAat 1031.853 kb on - strand, within hyaAat 1031.853 kb on - strand, within hyaAat 1032.036 kb on + strand, within hyaAat 1032.165 kb on + strand, within hyaAat 1032.232 kb on + strand, within hyaAat 1032.241 kb on + strand, within hyaAat 1032.283 kb on + strand, within hyaAat 1032.283 kb on + strand, within hyaAat 1032.409 kb on - strandat 1032.409 kb on - strandat 1032.419 kb on + strandat 1032.419 kb on + strandat 1032.474 kb on + strandat 1032.474 kb on + strandat 1032.527 kb on + strandat 1032.527 kb on + strandat 1032.721 kb on + strand, within hyaBat 1032.822 kb on - strand, within hyaBat 1032.822 kb on - strand, within hyaBat 1032.884 kb on - strand, within hyaBat 1032.940 kb on - strand, within hyaBat 1032.972 kb on - strand, within hyaBat 1033.010 kb on + strand, within hyaBat 1033.010 kb on + strand, within hyaBat 1033.093 kb on + strand, within hyaBat 1033.093 kb on + strand, within hyaBat 1033.093 kb on + strand, within hyaBat 1033.187 kb on + strand, within hyaBat 1033.214 kb on - strand, within hyaBat 1033.249 kb on + strand, within hyaBat 1033.249 kb on + strand, within hyaBat 1033.268 kb on + strand, within hyaBat 1033.268 kb on + strand, within hyaBat 1033.392 kb on - strand, within hyaBat 1033.419 kb on + strand, within hyaBat 1033.419 kb on + strand, within hyaBat 1033.517 kb on - strand, within hyaBat 1033.517 kb on - strand, within hyaBat 1033.529 kb on + strand, within hyaBat 1033.742 kb on + strand, within hyaBat 1033.742 kb on + strand, within hyaBat 1033.875 kb on - strand, within hyaBat 1033.960 kb on + strand, within hyaBat 1033.960 kb on + strand, within hyaBat 1034.059 kb on + strand, within hyaBat 1034.059 kb on + strand, within hyaBat 1034.191 kb on + strandat 1034.236 kb on - strandat 1034.252 kb on - strandat 1034.276 kb on + strandat 1034.276 kb on + strandat 1034.319 kb on + strandat 1034.319 kb on + strandat 1034.408 kb on - strand, within hyaCat 1034.577 kb on - strand, within hyaCat 1034.720 kb on - strand, within hyaCat 1034.720 kb on - strand, within hyaCat 1034.890 kb on - strand, within hyaCat 1034.895 kb on - strand, within hyaCat 1034.940 kb on + strandat 1034.940 kb on + strandat 1034.945 kb on + strandat 1034.947 kb on - strandat 1035.102 kb on + strand, within hyaDat 1035.201 kb on + strand, within hyaDat 1035.201 kb on + strand, within hyaD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ampicillin 0.00000195313 mM
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1,031,478 + hyaA b0972 0.10 +0.4
1,031,511 + hyaA b0972 0.13 -1.0
1,031,552 + hyaA b0972 0.17 -0.7
1,031,702 - hyaA b0972 0.30 +0.3
1,031,702 - hyaA b0972 0.30 -0.2
1,031,853 - hyaA b0972 0.44 -0.1
1,031,853 - hyaA b0972 0.44 -0.0
1,032,036 + hyaA b0972 0.60 +0.0
1,032,165 + hyaA b0972 0.72 +0.6
1,032,232 + hyaA b0972 0.78 -0.1
1,032,241 + hyaA b0972 0.79 -0.7
1,032,283 + hyaA b0972 0.82 -0.9
1,032,283 + hyaA b0972 0.82 -0.6
1,032,409 - -0.5
1,032,409 - +0.1
1,032,419 + +1.0
1,032,419 + -0.6
1,032,474 + -0.2
1,032,474 + +0.5
1,032,527 + +0.7
1,032,527 + +0.8
1,032,721 + hyaB b0973 0.14 -0.6
1,032,822 - hyaB b0973 0.19 +0.1
1,032,822 - hyaB b0973 0.19 +0.8
1,032,884 - hyaB b0973 0.23 +0.4
1,032,940 - hyaB b0973 0.26 -1.1
1,032,972 - hyaB b0973 0.28 +0.1
1,033,010 + hyaB b0973 0.30 +0.5
1,033,010 + hyaB b0973 0.30 -0.2
1,033,093 + hyaB b0973 0.34 +1.0
1,033,093 + hyaB b0973 0.34 -0.0
1,033,093 + hyaB b0973 0.34 +1.3
1,033,187 + hyaB b0973 0.40 -0.4
1,033,214 - hyaB b0973 0.41 +0.4
1,033,249 + hyaB b0973 0.43 -0.6
1,033,249 + hyaB b0973 0.43 -0.7
1,033,268 + hyaB b0973 0.44 -0.3
1,033,268 + hyaB b0973 0.44 +0.8
1,033,392 - hyaB b0973 0.51 +1.3
1,033,419 + hyaB b0973 0.53 +0.0
1,033,419 + hyaB b0973 0.53 +0.1
1,033,517 - hyaB b0973 0.58 +1.3
1,033,517 - hyaB b0973 0.58 -1.0
1,033,529 + hyaB b0973 0.59 +2.1
1,033,742 + hyaB b0973 0.71 +3.0
1,033,742 + hyaB b0973 0.71 +3.3
1,033,875 - hyaB b0973 0.78 +0.1
1,033,960 + hyaB b0973 0.83 -0.6
1,033,960 + hyaB b0973 0.83 -0.7
1,034,059 + hyaB b0973 0.88 -0.5
1,034,059 + hyaB b0973 0.88 +0.0
1,034,191 + -1.0
1,034,236 - +0.2
1,034,252 - +1.0
1,034,276 + -0.2
1,034,276 + -0.4
1,034,319 + -1.2
1,034,319 + -0.5
1,034,408 - hyaC b0974 0.17 +0.0
1,034,577 - hyaC b0974 0.41 -0.2
1,034,720 - hyaC b0974 0.61 +0.3
1,034,720 - hyaC b0974 0.61 +1.0
1,034,890 - hyaC b0974 0.85 +1.4
1,034,895 - hyaC b0974 0.86 -0.7
1,034,940 + -0.9
1,034,940 + +0.7
1,034,945 + -0.3
1,034,947 - +0.3
1,035,102 + hyaD b0975 0.19 -0.2
1,035,201 + hyaD b0975 0.35 -0.3
1,035,201 + hyaD b0975 0.35 -0.5

Or see this region's nucleotide sequence