Experiment: LB Anaerobic with Ampicillin 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaA and hyaB overlap by 4 nucleotides hyaB and hyaC are separated by 18 nucleotides hyaC and hyaD overlap by 4 nucleotides
b0972: hyaA - hydrogenase 1, small subunit (NCBI), at 1,031,362 to 1,032,480
hyaA
b0973: hyaB - hydrogenase 1, large subunit (NCBI), at 1,032,477 to 1,034,270
hyaB
b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996
hyaC
b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580
hyaD
Position (kb)
1032
1033
1034
1035 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1031.478 kb on + strand, within hyaA at 1031.511 kb on + strand, within hyaA at 1031.552 kb on + strand, within hyaA at 1031.702 kb on - strand, within hyaA at 1031.702 kb on - strand, within hyaA at 1031.853 kb on - strand, within hyaA at 1031.853 kb on - strand, within hyaA at 1032.036 kb on + strand, within hyaA at 1032.165 kb on + strand, within hyaA at 1032.232 kb on + strand, within hyaA at 1032.241 kb on + strand, within hyaA at 1032.283 kb on + strand, within hyaA at 1032.283 kb on + strand, within hyaA at 1032.409 kb on - strand at 1032.409 kb on - strand at 1032.419 kb on + strand at 1032.419 kb on + strand at 1032.474 kb on + strand at 1032.474 kb on + strand at 1032.527 kb on + strand at 1032.527 kb on + strand at 1032.721 kb on + strand, within hyaB at 1032.822 kb on - strand, within hyaB at 1032.822 kb on - strand, within hyaB at 1032.884 kb on - strand, within hyaB at 1032.940 kb on - strand, within hyaB at 1032.972 kb on - strand, within hyaB at 1033.010 kb on + strand, within hyaB at 1033.010 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.187 kb on + strand, within hyaB at 1033.214 kb on - strand, within hyaB at 1033.249 kb on + strand, within hyaB at 1033.249 kb on + strand, within hyaB at 1033.268 kb on + strand, within hyaB at 1033.268 kb on + strand, within hyaB at 1033.392 kb on - strand, within hyaB at 1033.419 kb on + strand, within hyaB at 1033.419 kb on + strand, within hyaB at 1033.517 kb on - strand, within hyaB at 1033.517 kb on - strand, within hyaB at 1033.529 kb on + strand, within hyaB at 1033.742 kb on + strand, within hyaB at 1033.742 kb on + strand, within hyaB at 1033.875 kb on - strand, within hyaB at 1033.960 kb on + strand, within hyaB at 1033.960 kb on + strand, within hyaB at 1034.059 kb on + strand, within hyaB at 1034.059 kb on + strand, within hyaB at 1034.191 kb on + strand at 1034.236 kb on - strand at 1034.252 kb on - strand at 1034.276 kb on + strand at 1034.276 kb on + strand at 1034.319 kb on + strand at 1034.319 kb on + strand at 1034.408 kb on - strand, within hyaC at 1034.577 kb on - strand, within hyaC at 1034.720 kb on - strand, within hyaC at 1034.720 kb on - strand, within hyaC at 1034.890 kb on - strand, within hyaC at 1034.895 kb on - strand, within hyaC at 1034.940 kb on + strand at 1034.940 kb on + strand at 1034.945 kb on + strand at 1034.947 kb on - strand at 1035.102 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ampicillin 0.00000195313 mM remove 1,031,478 + hyaA b0972 0.10 +0.4 1,031,511 + hyaA b0972 0.13 -1.0 1,031,552 + hyaA b0972 0.17 -0.7 1,031,702 - hyaA b0972 0.30 +0.3 1,031,702 - hyaA b0972 0.30 -0.2 1,031,853 - hyaA b0972 0.44 -0.1 1,031,853 - hyaA b0972 0.44 -0.0 1,032,036 + hyaA b0972 0.60 +0.0 1,032,165 + hyaA b0972 0.72 +0.6 1,032,232 + hyaA b0972 0.78 -0.1 1,032,241 + hyaA b0972 0.79 -0.7 1,032,283 + hyaA b0972 0.82 -0.9 1,032,283 + hyaA b0972 0.82 -0.6 1,032,409 - -0.5 1,032,409 - +0.1 1,032,419 + +1.0 1,032,419 + -0.6 1,032,474 + -0.2 1,032,474 + +0.5 1,032,527 + +0.7 1,032,527 + +0.8 1,032,721 + hyaB b0973 0.14 -0.6 1,032,822 - hyaB b0973 0.19 +0.1 1,032,822 - hyaB b0973 0.19 +0.8 1,032,884 - hyaB b0973 0.23 +0.4 1,032,940 - hyaB b0973 0.26 -1.1 1,032,972 - hyaB b0973 0.28 +0.1 1,033,010 + hyaB b0973 0.30 +0.5 1,033,010 + hyaB b0973 0.30 -0.2 1,033,093 + hyaB b0973 0.34 +1.0 1,033,093 + hyaB b0973 0.34 -0.0 1,033,093 + hyaB b0973 0.34 +1.3 1,033,187 + hyaB b0973 0.40 -0.4 1,033,214 - hyaB b0973 0.41 +0.4 1,033,249 + hyaB b0973 0.43 -0.6 1,033,249 + hyaB b0973 0.43 -0.7 1,033,268 + hyaB b0973 0.44 -0.3 1,033,268 + hyaB b0973 0.44 +0.8 1,033,392 - hyaB b0973 0.51 +1.3 1,033,419 + hyaB b0973 0.53 +0.0 1,033,419 + hyaB b0973 0.53 +0.1 1,033,517 - hyaB b0973 0.58 +1.3 1,033,517 - hyaB b0973 0.58 -1.0 1,033,529 + hyaB b0973 0.59 +2.1 1,033,742 + hyaB b0973 0.71 +3.0 1,033,742 + hyaB b0973 0.71 +3.3 1,033,875 - hyaB b0973 0.78 +0.1 1,033,960 + hyaB b0973 0.83 -0.6 1,033,960 + hyaB b0973 0.83 -0.7 1,034,059 + hyaB b0973 0.88 -0.5 1,034,059 + hyaB b0973 0.88 +0.0 1,034,191 + -1.0 1,034,236 - +0.2 1,034,252 - +1.0 1,034,276 + -0.2 1,034,276 + -0.4 1,034,319 + -1.2 1,034,319 + -0.5 1,034,408 - hyaC b0974 0.17 +0.0 1,034,577 - hyaC b0974 0.41 -0.2 1,034,720 - hyaC b0974 0.61 +0.3 1,034,720 - hyaC b0974 0.61 +1.0 1,034,890 - hyaC b0974 0.85 +1.4 1,034,895 - hyaC b0974 0.86 -0.7 1,034,940 + -0.9 1,034,940 + +0.7 1,034,945 + -0.3 1,034,947 - +0.3 1,035,102 + hyaD b0975 0.19 -0.2 1,035,201 + hyaD b0975 0.35 -0.3 1,035,201 + hyaD b0975 0.35 -0.5
Or see this region's nucleotide sequence