Experiment: LB Anaerobic with Ampicillin 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ssuB and ycbM overlap by 4 nucleotides ycbM and ssuD are separated by 10 nucleotides ssuD and ycbO overlap by 4 nucleotides ycbO and ssuE overlap by 8 nucleotides
b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267
ssuB
b0934: ycbM - putative transport system permease protein (VIMSS), at 993,264 to 994,055
ycbM
b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211
ssuD
b0936: ycbO - orf, hypothetical protein (VIMSS), at 995,208 to 996,167
ycbO
b0937: ssuE - NAD(P)H-dependent FMN reductase (NCBI), at 996,160 to 996,735
ssuE
Position (kb) 994
995
996 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 993.078 kb on - strand, within ssuB at 993.103 kb on + strand, within ssuB at 993.239 kb on - strand at 993.526 kb on + strand, within ycbM at 993.729 kb on - strand, within ycbM at 993.913 kb on + strand, within ycbM at 994.024 kb on + strand at 994.033 kb on - strand at 994.089 kb on + strand at 994.089 kb on + strand at 994.335 kb on - strand, within ssuD at 994.356 kb on + strand, within ssuD at 994.364 kb on - strand, within ssuD at 994.597 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.723 kb on + strand, within ssuD at 994.869 kb on + strand, within ssuD at 994.869 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.962 kb on + strand, within ssuD at 995.043 kb on - strand, within ssuD at 995.236 kb on - strand at 995.236 kb on - strand at 995.473 kb on - strand, within ycbO at 995.512 kb on + strand, within ycbO at 995.607 kb on + strand, within ycbO at 995.616 kb on + strand, within ycbO at 995.631 kb on + strand, within ycbO at 995.734 kb on - strand, within ycbO at 995.753 kb on - strand, within ycbO at 995.832 kb on - strand, within ycbO at 995.871 kb on + strand, within ycbO at 995.871 kb on + strand, within ycbO at 996.185 kb on + strand at 996.207 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ampicillin 0.00000195313 mM remove 993,078 - ssuB b0933 0.75 +0.1 993,103 + ssuB b0933 0.79 -0.2 993,239 - +0.7 993,526 + ycbM b0934 0.33 -0.5 993,729 - ycbM b0934 0.59 -1.0 993,913 + ycbM b0934 0.82 -0.2 994,024 + -0.8 994,033 - -0.0 994,089 + -2.3 994,089 + +0.8 994,335 - ssuD b0935 0.23 +2.9 994,356 + ssuD b0935 0.25 -0.9 994,364 - ssuD b0935 0.26 +0.6 994,597 - ssuD b0935 0.46 -0.8 994,710 - ssuD b0935 0.56 +2.7 994,710 - ssuD b0935 0.56 +1.4 994,723 + ssuD b0935 0.57 -0.1 994,869 + ssuD b0935 0.70 -0.9 994,869 - ssuD b0935 0.70 +0.8 994,900 - ssuD b0935 0.73 -1.3 994,900 - ssuD b0935 0.73 -0.2 994,962 + ssuD b0935 0.78 -0.3 995,043 - ssuD b0935 0.85 +0.6 995,236 - +1.2 995,236 - -0.3 995,473 - ycbO b0936 0.28 -0.3 995,512 + ycbO b0936 0.32 -1.5 995,607 + ycbO b0936 0.42 -0.3 995,616 + ycbO b0936 0.42 +0.3 995,631 + ycbO b0936 0.44 -0.1 995,734 - ycbO b0936 0.55 +0.6 995,753 - ycbO b0936 0.57 -1.0 995,832 - ycbO b0936 0.65 +0.9 995,871 + ycbO b0936 0.69 -1.2 995,871 + ycbO b0936 0.69 -0.2 996,185 + +0.4 996,207 - -0.6
Or see this region's nucleotide sequence