Strain Fitness in Escherichia coli BW25113 around b2814

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmltA and metZ are separated by 208 nucleotidesmetZ and metW are separated by 33 nucleotidesmetW and metV are separated by 33 nucleotidesmetV and b2817 are separated by 73 nucleotides b2813: mltA - membrane-bound lytic murein transglycosylase A (NCBI), at 2,944,103 to 2,945,200 mltA b2814: metZ - tRNA-Met (NCBI), at 2,945,409 to 2,945,485 metZ b2815: metW - tRNA-Met (NCBI), at 2,945,519 to 2,945,595 metW b2816: metV - tRNA-Met (NCBI), at 2,945,629 to 2,945,705 metV b2817: b2817 - putative amidase (VIMSS), at 2,945,779 to 2,947,032 b2817 Position (kb) 2945 2946Strain fitness (log2 ratio) -2 -1 0 1 2at 2944.433 kb on + strand, within mltAat 2944.433 kb on + strand, within mltAat 2944.455 kb on + strand, within mltAat 2944.485 kb on - strand, within mltAat 2944.485 kb on - strand, within mltAat 2944.553 kb on + strand, within mltAat 2944.553 kb on + strand, within mltAat 2944.561 kb on - strand, within mltAat 2944.564 kb on + strand, within mltAat 2944.566 kb on - strand, within mltAat 2944.569 kb on + strand, within mltAat 2944.652 kb on + strand, within mltAat 2944.736 kb on - strand, within mltAat 2944.860 kb on + strand, within mltAat 2944.860 kb on + strand, within mltAat 2944.883 kb on - strand, within mltAat 2944.883 kb on - strand, within mltAat 2944.943 kb on + strand, within mltAat 2944.943 kb on + strand, within mltAat 2944.943 kb on + strand, within mltAat 2945.034 kb on + strand, within mltAat 2945.034 kb on + strand, within mltAat 2945.094 kb on - strandat 2945.105 kb on + strandat 2945.105 kb on + strandat 2945.124 kb on + strandat 2945.184 kb on + strandat 2945.272 kb on + strandat 2945.423 kb on + strand, within metZat 2945.508 kb on - strandat 2946.029 kb on + strand, within b2817at 2946.029 kb on + strand, within b2817at 2946.068 kb on - strand, within b2817at 2946.068 kb on - strand, within b2817at 2946.086 kb on + strand, within b2817at 2946.094 kb on + strand, within b2817at 2946.164 kb on + strand, within b2817at 2946.172 kb on - strand, within b2817at 2946.213 kb on + strand, within b2817at 2946.223 kb on + strand, within b2817at 2946.223 kb on + strand, within b2817at 2946.267 kb on + strand, within b2817at 2946.309 kb on + strand, within b2817at 2946.334 kb on - strand, within b2817

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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2,944,433 + mltA b2813 0.30 -1.0
2,944,433 + mltA b2813 0.30 +1.0
2,944,455 + mltA b2813 0.32 +1.9
2,944,485 - mltA b2813 0.35 +1.2
2,944,485 - mltA b2813 0.35 -0.2
2,944,553 + mltA b2813 0.41 +0.9
2,944,553 + mltA b2813 0.41 -0.8
2,944,561 - mltA b2813 0.42 +0.8
2,944,564 + mltA b2813 0.42 +0.1
2,944,566 - mltA b2813 0.42 -0.4
2,944,569 + mltA b2813 0.42 +0.4
2,944,652 + mltA b2813 0.50 +0.1
2,944,736 - mltA b2813 0.58 -1.2
2,944,860 + mltA b2813 0.69 -2.0
2,944,860 + mltA b2813 0.69 +0.3
2,944,883 - mltA b2813 0.71 +1.0
2,944,883 - mltA b2813 0.71 +0.5
2,944,943 + mltA b2813 0.77 -2.2
2,944,943 + mltA b2813 0.77 -1.2
2,944,943 + mltA b2813 0.77 -0.6
2,945,034 + mltA b2813 0.85 +0.9
2,945,034 + mltA b2813 0.85 +1.7
2,945,094 - +0.3
2,945,105 + +0.6
2,945,105 + +0.3
2,945,124 + +0.2
2,945,184 + +0.9
2,945,272 + +0.0
2,945,423 + metZ b2814 0.18 +0.8
2,945,508 - +0.1
2,946,029 + b2817 b2817 0.20 -0.3
2,946,029 + b2817 b2817 0.20 +0.3
2,946,068 - b2817 b2817 0.23 -0.1
2,946,068 - b2817 b2817 0.23 -0.3
2,946,086 + b2817 b2817 0.24 +1.0
2,946,094 + b2817 b2817 0.25 -0.5
2,946,164 + b2817 b2817 0.31 +1.3
2,946,172 - b2817 b2817 0.31 +0.1
2,946,213 + b2817 b2817 0.35 -0.9
2,946,223 + b2817 b2817 0.35 -1.3
2,946,223 + b2817 b2817 0.35 -0.7
2,946,267 + b2817 b2817 0.39 +1.5
2,946,309 + b2817 b2817 0.42 +0.2
2,946,334 - b2817 b2817 0.44 +0.2

Or see this region's nucleotide sequence